scholarly journals Salmonella Enteritidis Isolate Harboring Multiple Efflux Pumps and Pathogenicity Factors, Shows Absence of O Antigen Polymerase Gene

2016 ◽  
Vol 7 ◽  
Author(s):  
Daniela Jones-Dias ◽  
Lurdes Clemente ◽  
Conceição Egas ◽  
Hugo Froufe ◽  
Daniel A. Sampaio ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Efflux Pumps ◽  
Polymerase Gene ◽  
Pathogenicity Factors ◽  
O Antigen

scholarly journals Molecular typing ofLeptospiraspp. based on putative O-antigen polymerase gene (wzy), the benefit over 16S rRNA gene sequence

2007 ◽  
Vol 271 (2) ◽  
pp. 170-179 ◽  
Author(s):  
Piyada Wangroongsarb ◽  
Teerarut Chanket ◽  
Kata Gunlabun ◽  
Do Hoang Long ◽  
Pedcharat Satheanmethakul ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Typing ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Polymerase Gene ◽  
Rrna Gene Sequence ◽  
O Antigen

scholarly journals Coexistence of Two Distinct Versions of O-Antigen Polymerase, Wzy-Alpha and Wzy-Beta, in Pseudomonas aeruginosa Serogroup O2 and Their Contributions to Cell Surface Diversity

2007 ◽  
Vol 189 (11) ◽  
pp. 4141-4152 ◽  
Author(s):  
Katarina Kaluzny ◽  
Priyanka D. Abeyrathne ◽  
Joseph S. Lam
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Surface ◽  
Nucleotide Sequencing ◽  
Polymerase Gene ◽  
Knockout Mutant ◽  
Sequence Identity ◽  
O Antigen ◽  
Reverse Transcription Pcr ◽  
Topo Cloning ◽  
Dependent Pathway

ABSTRACT Assembly of B-band lipopolysaccharide (LPS) in Pseudomonas aeruginosa follows a Wzy-dependent pathway, requiring the O-antigen polymerase Wzy and other proteins. The peptide sequences of the wzy α product from strains of serotypes O2, O5, and O16 are identical, but the O units in O5 are α-glycosidically linked, while those in O2 and O16 are β-linked. We hypothesized that a derivative of the D3 bacteriophage wzy β is present in the chromosomes of O2 and O16 and that this gene is responsible for the β-linkage. By a combination of PCR and primer walking, wzy β genes of both serotypes have been amplified and cloned. They are identical but share only 87.42% sequence identity with their xenolog in D3. A chromosomal knockout mutant of O16 wzy β was made, and it produces semirough LPS devoid of B-band O antigen. The cloned wzy β is capable of complementing the O16 wzy β mutant, as well as cross-complementing a wzy α knockout mutant. However, in the latter case, the restored O antigen was β-linked. Using reverse transcription-PCR, we showed that wzy α was transcribed in O2 and O16 strains and was functional, since both of these genes could complement the wzy α mutant of O5. With the coexistence of wzy α and wzy β in O2 and O16 and the B-band O polysaccharides in these being β-linked, we hypothesized that iap, an inhibitor of the alpha-polymerase gene, must be present in these serotypes. Indeed, through PCR, TOPO-cloning, and nucleotide-sequencing results, we verified the presence of iap in both O2 and O16 serotypes.

Interaction of a Salmonella enteritidis O-antigen octasaccharide with the phage P22 tailspike protein by NMR spectroscopy and docking studies

2007 ◽  
Vol 25 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Jens Landström ◽  
Eva-Lisa Nordmark ◽  
Robert Eklund ◽  
Andrej Weintraub ◽  
Robert Seckler ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Salmonella Enteritidis ◽  
Docking Studies ◽  
O Antigen ◽  
Phage P22 ◽  
P22 Tailspike ◽  
Tailspike Protein

scholarly journals Role of CpxR in Biofilm Development: Expression of Key Fimbrial, O-Antigen and Virulence Operons of Salmonella Enteritidis

2019 ◽  
Vol 20 (20) ◽  
pp. 5146
Author(s):  
Deeksha Shetty ◽  
Juan Abrahante ◽  
Samuel Chekabab ◽  
Xuxiaochen Wu ◽  
Darren Korber ◽  
...  
Keyword(s):  
Sigma Factor ◽  
Salmonella Enteritidis ◽  
Constitutive Expression ◽  
Microtiter Plate ◽  
Two Component System ◽  
Swarming Motility ◽  
O Antigen ◽  
Wild Type Counterpart ◽  
Health Significance ◽  
Biofilm Development

Salmonella Enteritidis is a non-typhoidal serovar of great public health significance worldwide. The RpoE sigma factor and CpxRA two-component system are the major regulators of the extracytoplasmic stress response. In this study, we found that the CpxR has highly significant, but opposite effects on the auto-aggregation and swarming motility of S. Enteritidis. Auto-aggregation was negatively affected in the ∆cpxR mutant, whereas the same mutant significantly out-performed its wild-type counterpart with respect to swarming motility, indicating that the CpxR plays a role in biofilm-associated phenotypes. Indeed, biofilm-related assays showed that the CpxR is of critical importance in biofilm development under both static (microtiter plate) and dynamic (flow cell) media flow conditions. In contrast, the RpoE sigma factor showed no significant role in biofilm development under dynamic conditions. Transcriptomic analysis revealed that the cpxR mutation negatively affected the constitutive expression of the operons critical for biosynthesis of O-antigen and adherence, but positively affected the expression of virulence genes critical for Salmonella-mediated endocytosis. Conversely, CpxR induced the expression of curli csgAB and fimbrial stdAC operons only during biofilm development and flagellar motAB and fliL operons exclusively during the planktonic phase, indicating a responsive biofilm-associated loop of the CpxR regulator.

scholarly journals Molecular Characterization of Cronobacter Lipopolysaccharide O-Antigen Gene Clusters and Development of Serotype-Specific PCR Assays

2011 ◽  
Vol 77 (12) ◽  
pp. 4017-4026 ◽  
Author(s):  
K. G. Jarvis ◽  
C. J. Grim ◽  
A. A. Franco ◽  
G. Gopinath ◽  
V. Sathyamoorthy ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Gene Clusters ◽  
Enterobacter Sakazakii ◽  
Polymerase Gene ◽  
Content Type ◽  
Antigen Gene ◽  
O Antigen ◽  
Specific Pcr ◽  
Pcr Assays

ABSTRACTCronobacter(formerlyEnterobacter sakazakii) is a recently defined genus consisting of six species,C. sakazakii,C. malonaticus,C. dublinensis,C. muytjensii,C. turicensis, andCronobactergenomospecies 1. In this study, MboII restriction fragment length polymorphism (RFLP) patterns of O-antigen gene clusters, located betweengalFandgnd, were used to identify serotypes inCronobacterspp. Seven O-antigen RFLP clusters were generated, including threeC. sakazakiiclusters, previously identified as serotypes O1, O2, and O3. The O-antigen regions of six strains with unique RFLP patterns, including twoC. sakazakiistrains, twoC. malonaticusstrains, oneC. turicensisstrain, and oneC. muytjensiistrain, revealed three O-antigen gene clusters shared amongCronobacterspecies. PCR assays were developed, targeting thewzxO-antigen polymerase gene, and used to screen 231Cronobacterstrains to determine the frequency of these newly identified serotypes.

scholarly journals Synthetic and immunological studies of Salmonella Enteritidis O-antigen tetrasaccharides as potential anti-Salmonella vaccines

2019 ◽  
Vol 55 (31) ◽  
pp. 4519-4522 ◽  
Author(s):  
Chang-Xin Huo ◽  
Debashis Dhara ◽  
Scott M. Baliban ◽  
Setare Tahmasebi Nick ◽  
Zibin Tan ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Complete Protection ◽  
O Antigen

The conjugate of a synthetic Salmonella Enteritidis tetrasaccharide with bacteriophage Qβ induced powerful anti-glycan IgG responses for complete protection from lethal challenges of bacteria.

scholarly journals Relationships among the O-Antigen Gene Clusters ofSalmonella enterica Groups B, D1, D2, and D3

1998 ◽  
Vol 180 (4) ◽  
pp. 1002-1007 ◽  
Author(s):  
Heather Curd ◽  
Dan Liu ◽  
Peter R. Reeves
Keyword(s):  
Cell Wall ◽  
Gene Cluster ◽  
Salmonella Enterica ◽  
Sequence Similarity ◽  
Gene Clusters ◽  
Gram Negative Bacteria ◽  
Polymerase Gene ◽  
Gram Negative ◽  
Antigen Gene ◽  
O Antigen

ABSTRACT The O antigen is an important cell wall antigen of gram-negative bacteria, and the genes responsible for its biosynthesis are located in a gene cluster. We have cloned and sequenced the DNA segment unique to the O-antigen gene cluster of Salmonella enterica group D3. This segment includes a novel O-antigen polymerase gene (wzy D3). The polymerase gives α(1→6) linkages but has no detectable sequence similarity to that of group D2, which confers the same linkage. We find the remnant of a D3-likewzy gene in the O-antigen gene clusters of groups D1 and B and suggest that this is the original wzy gene of these O-antigen gene clusters.

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