scholarly journals Optimization of Environmental Conditions for Microbial Stabilization of Uranium Tailings, and the Microbial Community Response

2021 ◽  
Vol 12 ◽  
Author(s):  
Ying Lv ◽  
Chuiyun Tang ◽  
Xingyu Liu ◽  
Mingjiang Zhang ◽  
Bowei Chen ◽  
...  
Keyword(s):  
Microbial Community ◽  
Sulfate Reducing Bacteria ◽  
Community Response ◽  
Phosphate Solubilizing Bacteria ◽  
Iron Reducing Bacteria ◽  
Sulfate Reducing ◽  
Effects Of Ph ◽  
Phosphate Solubilizing ◽  
Reducing Bacteria ◽  
Uranium Tailings

Uranium pollution in tailings and its decay products is a global environmental problem. It is of great significance to use economical and efficient technologies to remediate uranium-contaminated soil. In this study, the effects of pH, temperature, and inoculation volume on stabilization efficiency and microbial community response of uranium tailings were investigated by a single-factor batch experiment in the remediation process by mixed sulfate-reducing bacteria (SRB) and phosphate-solubilizing bacteria (PSB, Pantoea sp. grinm-12). The results showed that the optimal parameters of microbial stabilization by mixed SRB-PSB were pH of 5.0, temperature of 25°C, and inoculation volume of 10%. Under the optimal conditions, the uranium in uranium tailings presented a tendency to transform from the acid-soluble state to residual state. In addition, the introduction of exogenous SRB-PSB can significantly increase the richness and diversity of endogenous microorganisms, effectively maintain the reductive environment for the microbial stabilization system, and promote the growth of functional microorganisms, such as sulfate-reducing bacteria (Desulfosporosinus and Desulfovibrio) and iron-reducing bacteria (Geobacter and Sedimentibacter). Finally, PCoA and CCA analyses showed that temperature and inoculation volume had significant effects on microbial community structure, and the influence order of the three environmental factors is as follows: inoculation volume > temperature > pH. The outcomes of this study provide theoretical support for the control of uranium in uranium-contaminated sites.

Response of sulfate-reducing bacteria and supporting microbial community to persulfate exposure in a continuous flow system

2019 ◽  
Vol 21 (7) ◽  
pp. 1193-1203 ◽  
Author(s):  
Christopher K. Bartlett ◽  
Robin M. Slawson ◽  
Neil R. Thomson
Keyword(s):  
Microbial Community ◽  
Continuous Flow ◽  
Flow System ◽  
Sulfate Reducing Bacteria ◽  
Continuous Flow System ◽  
Sulfate Reducing ◽  
System P ◽  
P Recovery ◽  
Community Profile ◽  
Reducing Bacteria

Recovery of BTEX biodegradation, sulfate-reducing bacteria and associated community profile following exposure to persulfate in a continuous flow system.

Sulfate-reducing bacteria in the microbial community of acidic drainage from a gold deposit tailing storage

Microbiology ◽  
2017 ◽  
Vol 86 (2) ◽  
pp. 286-288 ◽  
Author(s):  
A. V. Mardanov ◽  
A. V. Beletskii ◽  
D. A. Ivasenko ◽  
N. V. Pimenov ◽  
O. V. Karnachuk ◽  
...  
Keyword(s):  
Microbial Community ◽  
Gold Deposit ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Reducing Bacteria

The Disinfection Efficacy of Chlorine on Sulfate-Reducing Bacteria and Iron Bacteria in Water Supply Systems

2013 ◽  
Vol 316-317 ◽  
pp. 657-660
Author(s):  
Bei Meng Qi ◽  
Bei Jia Wang ◽  
Chen Guang Wu ◽  
Yi Xing Yuan
Keyword(s):  
Water Supply ◽  
Sulfate Reducing Bacteria ◽  
Metal Corrosion ◽  
Water Supply Systems ◽  
Supply Networks ◽  
Minimum Requirement ◽  
Iron Reducing Bacteria ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Supply Systems

Sulfate reducing bacteria (SRB) and iron reducing bacteria (IRB) that widely exist in water supply networks are the main microorganisms leading to metal corrosion in pipelines. Chlorine is widely used in drinking water supply systems. The concentration of chlorine with SRB declined rapidly after 10 mins and reached 0 mg/L finally whereas it decreased more slowly with IRB. If the concentration of chlorine is lower than 0.2mg/L, IRB cannot be sterilized. It indicates that at the end of water pipes where the concentration of chlorine is required to be 0.05mg/L, chlorine is not effective since the concentration is below the minimum requirement of removing IRB

scholarly journals CHARACTERIZATION OF SULFATE REDUCING BACTERIA FROM BIOFILM SULFATE REDUCTION AND CU PRECIPITATION BIOREACTOR (BATCH CULTURE).

2018 ◽  
Vol 2 (2) ◽  
pp. 1
Author(s):  
Tyas Nyonita Punjungsari
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Sulfate Reduction ◽  
Batch Culture ◽  
Microbial Community Structure ◽  
Sulfate Reducing Bacteria ◽  
Zeolite Surface ◽  
Sulfate Reducing ◽  
Reducing Bacteria

The biofilm is a microbial community structure formed on the zeolite surface in a sulfate reduction bioreactor and Cu deposition using a SRB consortium . The biofilm soluble microbial solvent is expected to have the capability in sulfate reduction and Cu deposition. Characterization of isolates is required for the optimization of pure culture . The aim of this study is to isolate and characterize the biofilm sulfate reducing bacteria in the sulfate reduction bioreactor and the precipitation of Cu in Batch Culture by a consortium of Sulfate Reducing Bacteria. The method used in this study cultivation was done by using postgate B medium, isolation was done by diluting biofilm on NaCl salt, bacteria grown on NB and postgate B media, characterization done by morphology and biochemistry. There were 3 isolates of B1 (Metylobacterium ), B3 ( Desulfucoccus ), and B2 ( Desulfobacter ). B3 ( Desulfococcus) has the best ability to reduce sulfate and Cu precipitation.Keywords : Sulfur Reducing Bacteria (SRB), Biofilm, Sulfate, Cu. Received: 26 August, 2017; Accepter: 10 September, 2017 

scholarly journals Mercury Methylation by Dissimilatory Iron-Reducing Bacteria

2006 ◽  
Vol 72 (12) ◽  
pp. 7919-7921 ◽  
Author(s):  
E. J. Kerin ◽  
C. C. Gilmour ◽  
E. Roden ◽  
M. T. Suzuki ◽  
J. D. Coates ◽  
...  
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Culture Conditions ◽  
Mercury Methylation ◽  
Iron Reducing Bacteria ◽  
Sulfate Reducing ◽  
Abiotic Controls ◽  
Reducing Bacteria

ABSTRACT The Hg-methylating ability of dissimilatory iron-reducing bacteria in the genera Geobacter, Desulfuromonas, and Shewanella was examined. All of the Geobacter and Desulfuromonas strains tested methylated mercury while reducing Fe(III), nitrate, or fumarate. In contrast, none of the Shewanella strains produced methylmercury at higher levels than abiotic controls under similar culture conditions. Geobacter and Desulfuromonas are closely related to known Hg-methylating sulfate-reducing bacteria within the Deltaproteobacteria.

scholarly journals Detection of sulfate-reducing bacteria as an indicator for successful mitigation of sulfide production

2021 ◽  
Author(s):  
Avishek Dutta ◽  
Fernando Valle ◽  
Thomas Goldman ◽  
Jeff Keating ◽  
Ellen Burke ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Critical Time ◽  
Sulfate Reducing Bacteria ◽  
Time Points ◽  
Sulfate Reducing ◽  
Nitrate Treatment ◽  
Reducing Bacteria ◽  
Planktonic Community

Sulfate-reducing bacteria (SRB) are one of the main sources of biogenic H 2 S generation in oil reservoirs. Excess H 2 S production in these systems leads to oil biosouring, which causes operational risks, health hazards and can increase the cost of refining crude oil. Nitrate salts are often added to the system to suppress sulfidogenesis. Because SRB populations can persist in biofilms even after nitrate treatment, identifying shifts in the sessile community is crucial for successful mitigation. However, sampling the sessile community is hampered by its inaccessibility. Here we use the results of a long-term (148 days) ex situ experiment to identify particular sessile community members from observations of the sample waste stream. Microbial community structure was determined for 731 samples across twenty bioreactors using 16S rRNA gene sequencing. By associating microbial community structure with specific steps in the mitigation process, we could distinguish between taxa associated with H 2 S production and mitigation. After initiation of nitrate treatment, certain SRB populations increased in the planktonic community during critical time points, indicating the dissociation of SRBs from the biofilm. Predicted relative abundances of the dissimilatory sulfate reduction pathway also increased during the critical time points. Here, by analyzing the planktonic community structure, we describe a general method that uses high-throughput amplicon sequencing, metabolic inferences, and cell abundance data to identify successful biofilm mitigation. We anticipate that our approach is also applicable to other systems where biofilms must be mitigated but cannot be easily sampled. Importance Microbial biofilms are commonly present in many industrial processes and can negatively impact performance and safety. Within the oil industry, subterranean biofilms cause biosouring with implications for oil quality, cost, occupational health, and the environment. Because these biofilms cannot be directly sampled, methods are needed to indirectly assess the success of mitigation measures. This study demonstrates how the planktonic microbial community can be used to assess the dissociation of SRB-containing biofilms. We found that an increase in the abundance of a specific SRB population in the effluent after nitrate treatment can be used as a potential indicator for the successful mitigation of biofilm-forming SRBs. Moreover, a method for determining critical time points for detecting potential indicators is suggested. This study expands our knowledge in improving mitigation strategies for biosouring and could have broader implications in other systems where biofilms lead to adverse consequences.

scholarly journals Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel)

2015 ◽  
Vol 12 (10) ◽  
pp. 2847-2860 ◽  
Author(s):  
I. Bar-Or ◽  
E. Ben-Dov ◽  
A. Kushmaro ◽  
W. Eckert ◽  
O. Sivan
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Methane Oxidation ◽  
Microbial Community Structure ◽  
Iron Reduction ◽  
Sulfate Reducing Bacteria ◽  
Lake Kinneret ◽  
Anaerobic Oxidation Of Methane ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Abstract. Microbial methane oxidation is the primary control on the emission of the greenhouse gas methane into the atmosphere. In terrestrial environments, aerobic methanotrophic bacteria are largely responsible for this process. In marine sediments, a coupling of anaerobic oxidation of methane (AOM) with sulfate reduction, often carried out by a consortium of anaerobic methanotrophic archaea (ANME) and sulfate-reducing bacteria, consumes almost all methane produced within those sediments. Motivated by recent evidence for AOM with iron(III) in Lake Kinneret sediments, the goal of the present study was to link the geochemical gradients in the lake porewater to the microbial community structure. Screening of archaeal 16S rRNA gene sequences revealed a shift from hydrogenotrophic to acetoclastic methanogens with depth. The observed changes in microbial community structure suggest possible direct and indirect mechanisms for the AOM coupled to iron reduction in deep sediments. The percentage of members of the Nitrospirales order increased with depth, suggesting their involvement in iron reduction together with Geobacter genus and "reverse methanogenesis". An indirect mechanism through sulfate and ANME seems less probable due to the absence of ANME sequences. This is despite the abundant sequences related to sulfate-reducing bacteria (Deltaproteobacteria) together with the occurrence of dsrA in the deep sediment that could indicate the production of sulfate (disproportionation) from S0 for sulfate-driven AOM. The presence of the functional gene pmoA in the deep anoxic sediment together with sequences related to Methylococcales suggests the existence of a second unexpected indirect pathway – aerobic methane oxidation pathway in an anaerobic environment.

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