scholarly journals Dual Regulation of Phosphatidylserine Decarboxylase Expression by Envelope Stress Responses

2021 ◽  
Vol 8 ◽  
Author(s):  
Yasmine Hassoun ◽  
Julia Bartoli ◽  
Astrid Wahl ◽  
Julie Pamela Viala ◽  
Emmanuelle Bouveret
Keyword(s):  
Stress Response ◽  
Stress Responses ◽  
Response Regulators ◽  
Two Component System ◽  
Component System ◽  
Basal Expression ◽  
Gene Coding ◽  
Envelope Stress ◽  
Two Component ◽  
Artificial Activation

Bacteria adapt to versatile environments by modulating gene expression through a set of stress response regulators, alternative Sigma factors, or two-component systems. Among the central processes that must be finely tuned is membrane homeostasis, including synthesis of phospholipids (PL). However, few genetic regulations of this process have been reported. We have previously shown that the gene coding the first step of PL synthesis is regulated by σE and ppGpp, and that the BasRS (PmrAB) two component system controls the expression of the DgkA PL recycling enzyme. The gene coding for phosphatidylserine decarboxylase, the last step in phosphatidylethanolamine synthesis is another gene in the PL synthesis pathway susceptible of stress response regulation. Indeed, psd appears in transcriptome studies of the σE envelope stress Sigma factor and of the CpxAR two component system. Interestingly, this gene is presumably in operon with mscM coding for a miniconductance mechanosensitive channel. In this study, we dissected the promoter region of the psd-mscM operon and studied its regulation by σE and CpxR. By artificial activation of σE and CpxRA stress response pathways, using GFP transcriptional fusion and western-blot analysis of Psd and MscM enzyme production, we showed that the operon is under the control of two distinct promoters. One is activated by σE, the second is activated by CpxRA and also responsible for basal expression of the operon. The fact that the phosphatidylethanolamine synthesis pathway is controlled by envelope stress responses at both its first and last steps might be important for adaptation of the membrane to envelope perturbations.

scholarly journals The BceABRS Four-Component System Regulates the Bacitracin-Induced Cell Envelope Stress Response in Streptococcus mutans

2010 ◽  
Vol 54 (9) ◽  
pp. 3895-3906 ◽  
Author(s):  
Jing Ouyang ◽  
Xiao-Lin Tian ◽  
Jennifer Versey ◽  
Alexander Wishart ◽  
Yung-Hua Li
Keyword(s):  
Stress Response ◽  
Streptococcus Mutans ◽  
Transcriptional Control ◽  
Cell Envelope ◽  
Genetic Locus ◽  
Binding Motif ◽  
Two Component System ◽  
Component System ◽  
Envelope Stress ◽  
Two Component

ABSTRACT Streptococcus mutans is known to be resistant to bacitracin, a cyclic polypeptide antibiotic produced by certain species of the genus Bacillus. This property is often exploited in the isolation of S. mutans strains from highly heterogeneous oral microflora. A genetic locus consisting of a four-gene operon, bceABRS (formerly mbrABCD), the component genes of which are homologous to Bacillus subtilis bceRS-bceAB (encoding a two-component system and an ABC transporter), is required for bacitracin resistance in S. mutans. Here we describe the identification of a DNA binding site for the BceR response regulator and its transcriptional control of the bceABRS operon in response to the presence of bacitracin. We provide evidence indicating that phosphorylated BceR binds directly to a conserved invert repeat located between bp −120 and −78 of the bceABRS promoter region and positively regulates expression of the bceABRS operon. We also demonstrate that sensing of bacitracin by the BceS histidine kinase requires the presence of an intact BceAB transporter, since deletion of either bceA or bceB abolishes BceRS-mediated bacitracin sensing. The results suggest that the BceAB transporter acts as a cosensor, together with the BceRS two-component system, for bacitracin perception in S. mutans. By searching the S. mutans genome databases, we have identified three additional genes that share the consensus BceR binding motif at their promoter regions. Our initial work has confirmed that expression of these genes is directly controlled by BceRS, indicating that the bceABRS operon, along with the three additional genes, forms the BceRS regulon in S. mutans. Taking these findings together, we conclude that BceABRS comprises a four-component system that plays an important role in stimulus sensing, signal transduction, the gene regulatory network, and substrate transport for the cell envelope stress response in S. mutans.

scholarly journals Salmonella entericaSerovar Typhimurium CpxRA Two-Component System Contributes to Gut Colonization inSalmonella-Induced Colitis

2018 ◽  
Vol 86 (7) ◽  
pp. e00280-18 ◽  
Author(s):  
Mayuka Fujimoto ◽  
Ryosuke Goto ◽  
Takeshi Haneda ◽  
Nobuhiko Okada ◽  
Tsuyoshi Miki
Keyword(s):  
Stress Response ◽  
Growth Media ◽  
Two Component System ◽  
Bacterial Gene ◽  
Component System ◽  
Content Type ◽  
Bacterial Gene Expression ◽  
Gut Colonization ◽  
Envelope Stress ◽  
Two Component

ABSTRACTSalmonella enterica, a common cause of diarrhea, has to colonize the gut lumen to elicit disease. In the gut, the pathogen encounters a vast array of environmental stresses that cause perturbations in the bacterial envelope. The CpxRA two-component system monitors envelope perturbations and responds by altering the bacterial gene expression profile. This allowsSalmonellato survive under such harmful conditions. Therefore, CpxRA activation is likely to contribute toSalmonellagut infection. However, the role of the CpxRA-mediated envelope stress response inSalmonella-induced diarrhea is unclear. Here, we show that CpxRA is dispensable for the induction of colitis byS. entericaserovar Typhimurium, whereas it is required for gut colonization. We prove that CpxRA is expressed during gut infection and that the presence of antimicrobial peptides in growth media activates the expression of CpxRA-regulated genes. In addition, we demonstrate that aS. Typhimurium strain lacking thecpxRAgene is able to cause colitis but is unable to continuously colonize the gut. Finally, we show that CpxRA-dependent gut colonization requires the host gut inflammatory response, while DegP, a CpxRA-regulated protease, is dispensable. Our findings reveal that the CpxRA-mediated envelope stress response plays a crucial role inSalmonellagut infection, suggesting that CpxRA might be a promising therapeutic target for infectious diarrhea.

scholarly journals The pneumococcal two-component system VisRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pneumocytes

2019 ◽  
Author(s):  
Nicolás M. Reinoso-Vizcaíno ◽  
Melina B. Cian ◽  
Paulo R. Cortes ◽  
Nadia B. Olivero ◽  
Mirelys Hernandez-Morfa ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Stress Response ◽  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Intracellular Survival ◽  
Bacterial Pathogenesis ◽  
Two Component System ◽  
Component System ◽  
Two Component

AbstractThe virus-bacterial synergism implicated in secondary bacterial infections caused by Streptococcus pneumoniae following infection with epidemic or pandemic influenza A virus (IAV) is well documented. However, the molecular mechanisms behind such synergism remain largely ill-defined. In pneumocytes infected with influenza A virus, subsequent infection with S. pneumoniae leads to enhanced pneumococcal intracellular survival. The pneumococcal two-component system VisRH appears essential for such enhanced survival. Through comparative transcriptomic analysis between the ΔvisR and wt strains, a list of 179 differentially expressed genes was defined. Among those, the clpL protein chaperone gene and the psaB Mn+2 transporter gene, which are involved in the stress response, are important in enhancing S. pneumoniae survival in influenza-infected cells. The ΔvisR, ΔclpL and ΔpsaB deletion mutants display increased susceptibility to acidic and oxidative stress and no enhancement of intracellular survival in IAV-infected pneumocyte cells. These results suggest that the VisRH two-component system senses IAV-induced stress conditions and controls adaptive responses that allow survival of S. pneumoniae in IAV-infected pneumocytes.Author summaryS. pneumoniae is an inhabitant of the human nasopharynx that is capable of causing a variety of infections contributing to an estimated 1.6 million deaths each year. Many of these deaths occur as result of secondary S. pneumoniae infections following seasonal or pandemic influenza. Although S. pneumoniae is considered a typical extracellular pathogen, an intracellular survival mechanism has been more recently recognized as significant in bacterial pathogenesis. The synergistic effects between influenza A and S. pneumoniae in secondary bacterial infection are well documented; however, the effects of influenza infections on intracellular survival of S. pneumoniae are ill-defined. Here, we provide evidence that influenza infection increases S. pneumoniae intracellular survival in pneumocytes. We demonstrate that the poorly understood VisRH signal transduction system in pneumococcus controls the expression of genes involved in the stress response that S. pneumoniae needs to increase intracellular survival in influenza A-infected pneumocytes. These findings have important implications for understanding secondary bacterial pathogenesis following influenza and for the treatment of such infections in influenza-stricken patients.

scholarly journals The CtrA Regulon of Rhodobacter sphaeroides Favors Adaptation to a Particular Lifestyle

2020 ◽  
Vol 202 (7) ◽  
Author(s):  
José Hernández-Valle ◽  
Alejandro Sanchez-Flores ◽  
Sebastian Poggio ◽  
Georges Dreyfus ◽  
Laura Camarena
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Stress Responses ◽  
Transcriptome Sequencing ◽  
Growth Conditions ◽  
Fine Tuning ◽  
Rna Seq ◽  
Two Component System ◽  
Component System ◽  
Content Type ◽  
Two Component

ABSTRACT Activation of the two-component system formed by CckA, ChpT, and CtrA (kinase, phosphotransferase, and response regulator, respectively) in Rhodobacter sphaeroides does not occur under the growth conditions commonly used in the laboratory. However, it is possible to isolate a gain-of-function mutant in CckA that turns the system on. Using massive parallel transcriptome sequencing (RNA-seq), we identified 321 genes that are differentially regulated by CtrA. From these genes, 239 were positively controlled and 82 were negatively regulated. Genes encoding the Fla2 polar flagella and gas vesicle proteins are strongly activated by CtrA. Genes involved in stress responses as well as several transcriptional factors are also positively controlled, whereas the photosynthetic and CO2 fixation genes are repressed. Potential CtrA-binding sites were bioinformatically identified, leading to the proposal that at least 81 genes comprise the direct regulon. Based on our results, we ponder that the transcriptional response orchestrated by CtrA enables a lifestyle in which R. sphaeroides will effectively populate the surface layer of a water body enabled by gas vesicles and will remain responsive to chemotactic stimuli using the chemosensoring system that controls the Fla2 flagellum. Simultaneously, fine-tuning of photosynthesis and stress responses will reduce the damage caused by heat and high light intensity in this water stratum. In summary, in this bacterium CtrA has evolved to control physiological responses that allow its adaptation to a particular lifestyle instead of controlling the cell cycle as occurs in other species. IMPORTANCE Cell motility in Alphaproteobacteria is frequently controlled by the CckA, ChpT, and CtrA two-component system. Under the growth conditions commonly used in the laboratory, ctrA is transcriptionally inactive in Rhodobacter sphaeroides, and motility depends on the Fla1 flagellar system that was acquired by a horizontal transfer event. Likely, the incorporation of this flagellar system released CtrA from the strong selective pressure of being the main motility regulator, allowing this two-component system to specialize and respond to some specific conditions. Identifying the genes that are directly regulated by CtrA could help us understand the conditions in which the products of this regulon are required. Massive parallel transcriptome sequencing (RNA-seq) revealed that CtrA orchestrates an adaptive response that contributes to the colonization of a particular environmental niche.

scholarly journals Two-Component System Cross-Regulation Integrates Bacillus anthracis Response to Heme and Cell Envelope Stress

2014 ◽  
Vol 10 (3) ◽  
pp. e1004044 ◽  
Author(s):  
Laura A. Mike ◽  
Jacob E. Choby ◽  
Paul R. Brinkman ◽  
Lorenzo Q. Olive ◽  
Brendan F. Dutter ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Cell Envelope ◽  
Two Component System ◽  
Component System ◽  
Envelope Stress ◽  
Two Component

scholarly journals GacA, the Response Regulator of a Two-Component System, Acts as a Master Regulator in Pseudomonas syringae pv. tomato DC3000 by Controlling Regulatory RNA, Transcriptional Activators, and Alternate Sigma Factors

2003 ◽  
Vol 16 (12) ◽  
pp. 1106-1117 ◽  
Author(s):  
Asita Chatterjee ◽  
Yaya Cui ◽  
Hailian Yang ◽  
Alan Collmer ◽  
James R. Alfano ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Stress Responses ◽  
Response Regulator ◽  
Sigma Factors ◽  
Regulatory Rna ◽  
Two Component System ◽  
Tomato Dc3000 ◽  
Component System ◽  
Regulatory Cascade ◽  
Two Component

Concerted investigations of factors affecting host-pathogen interactions are now possible with the model plant Arabidopsis thaliana and its model pathogen Pseudomo-nas syringae pv. tomato DC3000, as their whole genome sequences have become available. As a prelude to analysis of the regulatory genes and their targets, we have focused on GacA, the response regulator of a two-component system. The DC3000 gene was cloned by testing for the reversal of phenotypes of an Erwinia GacA− mutant. A GacA− mutant of DC3000 constructed by marker exchange produces much-reduced levels of transcripts of three alternate sigma factors: HrpL, required for the production of effector proteins and their translocation via the type III secretion system; RpoS, required for stress responses and secondary metabolite production; and RpoN, required for an assortment of metabolic processes and expression of hrpL. GacA deficiency also reduces the expression of hrpR and hrpS, which specify enhancer-binding proteins of the NtrC family required for hrpL transcription; ahlI and ahlR, the genes for quorum sensing signal; salA, a regulatory gene known to control virulence; CorS, a sensor kinase; CorR, the cognate response regulator that controls coronatine biosynthetic genes; and rsmB and rsmZ, which specify untranslatable regulatory RNA species. gacA expression itself is regulated by environmental conditions in DC3000, since transcript levels are affected by growth phase and media composition. The observations that high levels of gacA RNA occur in the hrp-inducing medium and GacA deficiency reduces the levels of rpoS expression implicate an important role of GacA in stress responses of DC3000. Consistent with the effects on hrpL expression, the GacA− mutant produces lower levels of transcripts of avr, hrp, and hop genes controlled by HrpL. In addition, GacA deficiency results in reduced levels of transcripts of several HrpL-independent genes. As would be expected, these effects on gene expression cause drastic changes in bacterial behavior: virulence towards A. thaliana and tomato; multiplication in planta; efficiency of the induction of the hypersensitive reaction (HR); production of pigment and N-acyl-homoserine lactone (AHL), the presumed quorum-sensing signal; and swarming motility. Our findings establish that GacA, located at the top in a regulatory cascade in DC3000, functions as a central regulator by controlling an assortment of transcriptional and posttranscriptional factors.

scholarly journals A Novel Three-Protein Two-Component System Provides a Regulatory Twist on an Established Circuit To Modulate Expression of the cbbI Region of Rhodopseudomonas palustris CGA010

2006 ◽  
Vol 188 (8) ◽  
pp. 2780-2791 ◽  
Author(s):  
Simona Romagnoli ◽  
F. Robert Tabita
Keyword(s):  
Response Regulator ◽  
Rhodopseudomonas Palustris ◽  
Sensor Kinase ◽  
Response Regulators ◽  
Transcription Activator ◽  
Two Component System ◽  
Bisphosphate Carboxylase ◽  
Control Of Gene Expression ◽  
Component System ◽  
Two Component

ABSTRACT A novel two-component system has been identified in the cbbI region of the nonsulfur purple photosynthetic bacterium Rhodopseudomonas palustris. Genes encoding this system, here designated cbbRRS, are juxtaposed between the divergently transcribed transcription activator gene, cbbR, and the form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) genes, cbbLS. The three genes of the cbbRRS system represent a variation of the well-known two-component signal transduction systems, as there are a transmembrane hybrid sensor kinase and two response regulators, with no apparent DNA binding domain associated with any of the three proteins encoded by these genes. In this study, we showed that the membrane-bound full-length kinase undergoes autophosphorylation and transfers phosphate to both response regulators. A soluble, truncated version of the kinase was subsequently prepared and found to catalyze phosphorylation of response regulator 1 but not response regulator 2, implying that conformational changes and/or sequence-specific regions of the kinase are important for discriminating between the two response regulators. Analyses indicated that a complex network of control of gene expression must occur, with CbbR required for the expression of the cbbLS genes but dispensable for the synthesis of form II RubisCO (encoded by cbbM). The CbbRRS proteins specifically affected the activity and accumulation of form I RubisCO (CbbLS), as revealed by analyses of nonpolar, unmarked gene deletions. A tentative model of regulation suggested that changes in the phosphotransfer activity of the sensor kinase, possibly in response to a redox metabolic signal, cause modulation of the activity and synthesis of form I RubisCO.

scholarly journals Counterbalancing Regulation in Response Memory of a Positively Autoregulated Two-Component System

2017 ◽  
Vol 199 (18) ◽  
Author(s):  
Rong Gao ◽  
Katherine A. Godfrey ◽  
Mahir A. Sufian ◽  
Ann M. Stock
Keyword(s):  
Stress Response ◽  
Natural Environments ◽  
Bacterial Cells ◽  
Two Component System ◽  
Experimental Conditions ◽  
Component System ◽  
Content Type ◽  
Regulatory Pathways ◽  
Pi Starvation ◽  
Two Component

ABSTRACT Fluctuations in nutrient availability often result in recurrent exposures to the same stimulus conditions. The ability to memorize the past event and use the “memory” to make adjustments to current behaviors can lead to a more efficient adaptation to the recurring stimulus. A short-term phenotypic memory can be conferred via carryover of the response proteins to facilitate the recurrent response, but the additional accumulation of response proteins can lead to a deviation from response homeostasis. We used the Escherichia coli PhoB/PhoR two-component system (TCS) as a model system to study how cells cope with the recurrence of environmental phosphate (Pi) starvation conditions. We discovered that “memory” of prior Pi starvation can exert distinct effects through two regulatory pathways, the TCS signaling pathway and the stress response pathway. Although carryover of TCS proteins can lead to higher initial levels of transcription factor PhoB and a faster initial response in prestarved cells than in cells not starved, the response enhancement can be overcome by an earlier and greater repression of promoter activity in prestarved cells due to the memory of the stress response. The repression counterbalances the carryover of the response proteins, leading to a homeostatic response whether or not cells are prestimulated. A computational model based on sigma factor competition was developed to understand the memory of stress response and to predict the homeostasis of other PhoB-regulated response proteins. Our insight into the history-dependent PhoBR response may provide a general understanding of how TCSs respond to recurring stimuli and adapt to fluctuating environmental conditions. IMPORTANCE Bacterial cells in their natural environments experience scenarios that are far more complex than are typically replicated in laboratory experiments. The architectures of signaling systems and the integration of multiple adaptive pathways have evolved to deal with such complexity. In this study, we examined the molecular “memory” that is generated by previous exposure to stimulus. Under our experimental conditions, activating effects of autoregulated two-component signaling and inhibitory effects of the stress response counterbalanced the transcriptional output to approach response homeostasis whether or not cells had been preexposed to stimulus. Modeling allows prediction of response behavior in different scenarios and demonstrates both the robustness of the system output and its sensitivity to historical parameters such as timing and levels of exposure to stimuli.

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