scholarly journals Cytotoxic T Cell Responses Induced by CS1/CRT Fusion DNA Vaccine in a Human Plasmacytoma Model

2020 ◽  
Vol 10 ◽  
Author(s):  
Xueshi Ye ◽  
Wanli Li ◽  
Jinwen Huang ◽  
Lifei Zhang ◽  
Ye Zhang
Keyword(s):  
Multiple Myeloma ◽  
T Cell ◽  
Dna Vaccine ◽  
T Cell Responses ◽  
Cytotoxic T Cell ◽  
Myeloma Cells ◽  
Cell Responses ◽  
Incurable Disease ◽  
Cytotoxic T Cell Responses

To date, multiple myeloma remains an incurable disease. Immunotherapy is an encouraging option in the development of multiple myeloma (MM) therapy. CS1 is a specific myeloma antigen, which is highly expressed in myeloma cells. Calreticulin (CRT) is a key determinant of cell death, which can influence antigen presentation and promote cellular phagocytic uptake. In the current study, we constructed a DNA vaccine encoding both CS1 and CRT. Our results show that the PcDNA3.1-CS1/CRT vaccine was able to induce cytotoxic T cell responses against myeloma cells in vivo, and the tumor growth was significantly suppressed in mice immunized with this vaccine. Therefore, our findings indicate that the CS1/CRT fusion DNA vaccine may represent a promising novel myeloma therapy, and the potential for combining the CS1/CRT vaccine with other myeloma treatments.

scholarly journals Influence of strong CD4 epitope on long-term virus-specific cytotoxic T cell responses induced in vivo with peptides

1996 ◽  
Vol 8 (4) ◽  
pp. 457-465 ◽  
Author(s):  
Jean-Pierre Sauzet ◽  
Helene Gras-Masse ◽  
Jean-Gerard Guillet ◽  
Elisabeth Gomard
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Cytotoxic T Cell ◽  
Cell Responses ◽  
Cytotoxic T Cell Responses

scholarly journals Laser facilitated epicutaneous peptide immunization using dry patch technology

2021 ◽  
Author(s):  
Sandra Scheiblhofer ◽  
Stephan Drothler ◽  
Werner Braun ◽  
Reinhard Braun ◽  
Maximilian Boesch ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Epitope ◽  
T Cell Responses ◽  
Cytotoxic T Cell ◽  
Target Tissue ◽  
Adjuvant Effect ◽  
Cell Responses ◽  
Dry Patch ◽  
Cytotoxic T Cell Responses

AbstractThe skin has been intensely investigated as a target tissue for immunization because it is populated by multiple types of antigen presenting cells. Directly addressing dendritic cells or Langerhans cells in vivo represents an attractive strategy for inducing T cell responses in cancer immunotherapy. We and others have studied fractional laser ablation as a novel method combining efficient delivery of macromolecules to the skin with an inherent adjuvant effect of laser illumination. In this proof of concept study, we demonstrate the feasibility of peptide delivery to the skin using the P.L.E.A.S.E. professional Erb:YAG fractional infrared laser together with EPIMMUN patches. In an ovalbumin mouse model we demonstrate that a dry patch formulation of SIINFEKL peptide in combination with CpG-ODN1826, but not imiquimod or polyI:C, induces potent cytotoxic T cell responses, which can be further boosted by co-delivery of the pan-helper T cell epitope PADRE.

scholarly journals The Extra Domain A from Fibronectin Targets Antigens to TLR4-Expressing Cells and Induces Cytotoxic T Cell Responses In Vivo

2007 ◽  
Vol 178 (2) ◽  
pp. 748-756 ◽  
Author(s):  
Juan J. Lasarte ◽  
Noelia Casares ◽  
Marta Gorraiz ◽  
Sandra Hervás-Stubbs ◽  
Laura Arribillaga ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Cytotoxic T Cell ◽  
Cell Responses ◽  
Cytotoxic T Cell Responses

scholarly journals CD103+ pulmonary dendritic cells preferentially acquire and present apoptotic cell–associated antigen

2011 ◽  
Vol 208 (9) ◽  
pp. 1789-1797 ◽  
Author(s):  
A. Nicole Desch ◽  
Gwendalyn J. Randolph ◽  
Kenneth Murphy ◽  
Emmanuel L. Gautier ◽  
Ross M. Kedl ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Apoptotic Cell ◽  
T Cell Responses ◽  
Cytotoxic T Cell ◽  
Soluble Antigen ◽  
Cell Responses ◽  
Inflammatory Conditions ◽  
Cytotoxic T Cell Responses

Cells undergoing programmed cell death (apoptosis) are removed in situ by macrophages and dendritic cells (DCs) through a specialized form of phagocytosis (efferocytosis). In the lung, there are two primary DC subsets with the potential to migrate to the local lymph nodes (LNs) and initiate adaptive immune responses. In this study, we show that only CD103+ DCs were able to acquire and transport apoptotic cells to the draining LNs and cross present apoptotic cell–associated antigen to CD8 T cells. In contrast, both the CD11bhi and the CD103+ DCs were able to ingest and traffic latex beads or soluble antigen. CD103+ DCs selectively exhibited high expression of TLR3, and ligation of this receptor led to enhanced in vivo cytotoxic T cell responses to apoptotic cell–associated antigen. The selective role for CD103+ DCs was confirmed in Batf3−/− mice, which lack this DC subtype. Our findings suggest that CD103+ DCs are the DC subset in the lung that captures and presents apoptotic cell–associated antigen under homeostatic and inflammatory conditions and raise the possibility for more focused immunological targeting to CD8 T cell responses.

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