Introgression of Blast Resistance Genes (Putative Pi-b and Pi-kh) into Elite Rice Cultivar MR219 through Marker-Assisted Selection

Grain productivity of rice is significantly reduced by dangerous disease – blast. Therefore, the development of resistant high yielding rice varieties with the Pi group of genes is important. Use of molecular markers linked with the loci of resistance significantly optimizes the breeding process. The purpose of the research is to develop rice lines combining 2-6 loci of resistance to blast: Pi-1, Pi-2, Pi-33, Pi-ta, Pi-b, Pi-40 by molecular marking. Materials and Methods. As donors of resistance genes foreign samples were used, recipient – Russian varieties. In the studies we used micro-satellites markers and PCR analysis. Results. In the first stage of the research as a result of hybridization domestic lines with genes Pi-l, Pi-2, Pi-33 were obtained. At the second stage – hybrids with all 3 genes were developed. In the third stage genes Pi-b and Pi-ta and on the fourth – Pi-40 were introduced. Conclusion. As a result, rice genotypes, combining 6 loci of blast resistance were developed with use of marker assisted selection.

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Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection

Czech Journal of Genetics and Plant Breeding ◽

10.17221/51/2016-cjgpb ◽

2017 ◽

Vol 53 (No. 1) ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

T. Suwannual ◽

S. Chankaew ◽

T. Monkham ◽

W. Saksirirat ◽

J. Sanitchon

Keyword(s):

Marker Assisted Selection ◽

Blast Resistance ◽

Rice Cultivar ◽

Introgression Lines ◽

Blast Disease ◽

Resistance Qtls ◽

Breeding Lines ◽

Rice Disease ◽

Blast Resistance Genes ◽

Yield Penalty

Thai rice cultivar RD6 is well known for its cooking and eating qualities. However it is susceptible to blast disease, a major rice disease caused by the fungus Magnaporthe oryzae. This study focused on the pyramiding of four QTLs for blast resistance located on chromosomes 1, 2, 11 and 12, from two RD6 introgression lines. Marker-assisted selection was performed and facilitated the selection with 8 microsatellite flanking markers to enable the selection in BC<sub>2</sub>F<sub>2:3</sub> lines. All possible combinations of the four QTL alleles were assessed for blast resistance by artificial inoculation using 8 diverse isolates in a greenhouse and under field conditions using the upland short row method. The results showed that the RD6 introgression lines carrying a high number of QTLs for blast resitance achieved from pyramiding have high levels of blast resistance and broad spectrum of resistance to the blast pathogens prevalent in the region. Only one of the M. oryzae isolates, THL185, was virulent to all the breeding lines, suggesting that the identification of new blast resistance genes or QTLs and pyramiding them into RD6 for durable blast resistance and no yield penalty should be the focus of further research.

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Molecular Mapping of the Blast Resistance Genes Pi2-1 and Pi51(t) in the Durably Resistant Rice ‘Tianjingyeshengdao’

Phytopathology ◽

10.1094/phyto-03-12-0042-r ◽

2012 ◽

Vol 102 (8) ◽

pp. 779-786 ◽

Cited By ~ 19

Author(s):

Yue Wang ◽

Dan Wang ◽

Xiaojuan Deng ◽

Jinling Liu ◽

Pingyong Sun ◽

...

Keyword(s):

Resistance Genes ◽

Phenotypic Variation ◽

Molecular Mapping ◽

Blast Resistance ◽

Recombinant Inbred Lines ◽

Durable Resistance ◽

Rice Cultivar ◽

Breeding Programs ◽

Rice Blast Resistance ◽

Blast Resistance Genes

Tianjingyeshengdao' (TY) is a rice cultivar with durable resistance to populations of Magnaporthe oryzae (the causal agent of blast) in China. To understand the genetic basis of its resistance to blast, we developed a population of recombinant inbred lines from a cross between TY and the highly susceptible ‘CO39’ for gene mapping analysis. In total, 22 quantitative trait loci (QTLs) controlling rice blast resistance were identified on chromosomes 1, 3, 4, 5, 6, 9, 11, and 12 from the evaluation of four disease parameters in both greenhouse and blast nursery conditions. Among these QTLs, 19 were contributed by TY and three by CO39. Two QTL clusters on chromosome 6 and 12 were named Pi2-1 and Pi51(t), respectively. Pi2-1 was detected under both growth chamber and natural blast nursery conditions, and explained 31.24 to 59.73% of the phenotypic variation. Pi51(t) was only detected in the natural blast nursery and explained 3.67 to 10.37% of the phenotypic variation. Our results demonstrate that the durable resistance in TY is controlled by two major and seven minor genes. Identification of the markers linked to both Pi2-1 and Pi51(t) in this study should be useful for marker-aided selection in rice breeding programs as well as for molecular cloning of the identified resistance genes.

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Identification and fine mapping of two blast resistance genes in rice cultivar 93-11

The Crop Journal ◽

10.1016/j.cj.2013.07.007 ◽

2013 ◽

Vol 1 (1) ◽

pp. 2-14 ◽

Cited By ~ 18

Author(s):

Cailin Lei ◽

Kun Hao ◽

Yilong Yang ◽

Jian Ma ◽

Shuai Wang ◽

...

Keyword(s):

Resistance Genes ◽

Fine Mapping ◽

Blast Resistance ◽

Rice Cultivar ◽

Blast Resistance Genes

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Marker-assisted introgression of three dominant blast resistance genes into an aromatic rice cultivar Mushk Budji

Scientific Reports ◽

10.1038/s41598-018-22246-4 ◽

2018 ◽

Vol 8 (1) ◽

Cited By ~ 6

Author(s):

Gazala Hassan Khan ◽

Asif Bashir Shikari ◽

Rakesh Vaishnavi ◽

Sofi Najeeb ◽

Bilal A. Padder ◽

...

Keyword(s):

Resistance Genes ◽

Blast Resistance ◽

Rice Cultivar ◽

Aromatic Rice ◽

Blast Resistance Genes

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Effects of Pyramiding Quantitative Resistance Genes pi21, Pi34, and Pi35 on Rice Leaf Blast Disease

Plant Disease ◽

10.1094/pdis-02-14-0214-re ◽

2015 ◽

Vol 99 (7) ◽

pp. 904-909 ◽

Cited By ~ 26

Author(s):

Nobuko Yasuda ◽

Takayuki Mitsunaga ◽

Keiko Hayashi ◽

Shinzo Koizumi ◽

Yoshikatsu Fujita

Keyword(s):

Resistance Genes ◽

Blast Resistance ◽

Quantitative Resistance ◽

Durable Resistance ◽

Rice Cultivar ◽

Infection Process ◽

Blast Disease ◽

Leaf Blast ◽

Gene Combination ◽

Blast Resistance Genes

Development of resistant cultivars has been an effective method for controlling rice blast disease caused by Magnaporthe oryzae. Quantitative blast resistance genes may offer durable resistance because the selection pressure on M. oryzae to overcome resistance is low as a result of the genes’ moderate susceptibility. Because the effects of individual resistance genes are relatively small, pyramiding these genes in rice cultivars is a promising strategy. Here, we used near-isogenic and backcross lines of rice cultivar Koshihikari with single- or two-gene combinations of blast resistance genes (pi21, Pi34, and Pi35) to evaluate the suppression of leaf blast. The severity of the disease was assessed throughout the infection process. Resistance varied among the lines: Pi35 conferred the strongest resistance, while Pi34 showed the weakest effects. Two types of combined-gene interactions were observed, and they varied on the basis of gene combination and characteristic of the infection: (i) the combination of two resistance genes was more effective than either of the genes individually or (ii) the combination of two resistance genes was similar to the level of the most effective resistance gene in the pair. The most effective gene combination for the suppression of leaf blast was pi21 + Pi35.

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