The Winter-Type Allele of HvCEN Is Associated With Earliness Without Severe Yield Penalty in Icelandic Spring Barley (Hordeum vulgare L.)

Icelandic barley genotypes have shown extreme earliness both in flowering and maturity compared to other north European genotypes, whereas earliness is a key trait in adapting barley to northern latitudes. Four genes were partially re-sequenced, which are Ppd-H1, HvCEN, HvELF3, and HvFT1, to better understand the mechanisms underlying this observed earliness. These genes are all known to play a part in the photoperiod response. The objective of this study is to correlate allelic diversity with flowering time and yield data from Icelandic field trials. The resequencing identified two to three alleles at each locus which resulted in 12 haplotype combinations. One haplotype combination containing the winter-type allele of Ppd-H1 correlated with extreme earliness, however, with a severe yield penalty. A winter-type allele in HvCEN in four genotypes correlated with earliness combined with high yield. Our results open the possibility of marker-assisted pyramiding as a rapid way to develop varieties with a shortened time from sowing to flowering under the extreme Icelandic growing conditions and possibly in other arctic or sub-arctic regions.

Marker-assisted forward and backcross breeding for improvement of elite Indian rice variety Naveen for multiple biotic and abiotic stress tolerance

The elite Indian rice variety, Naveen is highly susceptible to major biotic and abiotic stresses such as blast, bacterial blight (BB), gall midge (GM) and drought which limit its productivity in rainfed areas. In the present study, a combined approach of marker-assisted forward (MAFB) and back cross (MABC) breeding was followed to introgress three major genes, viz., Pi9 for blast, Xa21 for bacterial blight (BB), and Gm8 for gall midge (GM) and three major QTLs, viz., qDTY1.1, qDTY2.2 and qDTY4.1 conferring increased yield under drought in the background of Naveen. At each stage of advancement, gene-based/linked markers were used for the foreground selection of biotic and abiotic stress tolerant genes/QTLs. Intensive phenotype-based selections were performed in the field for identification of lines with high level of resistance against blast, BB, GM and drought tolerance without yield penalty under non-stress situation. A set of 8 MAFB lines and 12 MABC lines with 3 to 6 genes/QTLs and possessing resistance/tolerance against biotic stresses and reproductive stage drought stress with better yield performance compared to Naveen were developed. Lines developed through combined MAFB and MABC performed better than lines developed only through MAFB. This study exemplifies the utility of the combined approach of marker-assisted forward and backcrosses breeding for targeted improvement of multiple biotic and abiotic stress resistance in the background of popular mega varieties.

Institutional analysis defining the crucial factors for scaling low-emissions rice production: Evidence from Bangladesh

This article provides a comprehensive institutional analysis to better understand the key conditions and incentives relevant for the uptake of alternate wetting and drying (AWD) – a climate-smart irrigation practice in Bangladesh. Practicing AWD in rice production can reduce water consumption by 30% and mitigate methane emissions by 30-70% without yield penalty. Primary research was conducted in Rangpur, Rajshahi, Mymensingh, and Sherpur Divisions. The analysis depicts the dynamic among the stakeholders involved in outscaling AWD using the Net-Map tool, and demonstrates the influence of irrigation management systems in the selected catchment areas and the impacts of AWD in rice farming. Findings reveal that AWD-practicing farmers, pump owners, and the Upazila Irrigation Committee are the three most powerful influencers for outscaling AWD. The pre-paid irrigation system significantly improves water efficiency and cost-effectiveness when practising AWD. Additionally, AWD leads to a lower frequency of pumping resulting in reduced costs compared to the continuous-flooding practice. Finally, the results guide the formulation of recommendations to create favourable conditions for AWD outscaling, which contributes to reducing greenhouse gas emissions and advancing sustainable rice production in Bangladesh.

Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought

Background The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions.

Development of white-grain pre-harvest sprouting tolerant and pyramided protein-rich leaf rust resistant wheats using molecular breeding

The present study was undertaken for developing pre-harvest sprouting tolerant (PHST) wheat genotypes using marker-assisted backcross breeding (MABB). A major QTL for PHST was introgressed into an elite Indian wheat cv. Lok1 that is PHS susceptible. These PHST lines were also pyramided with one gene each for high grain protein content (Gpc-B1) and leaf rust resistance (Lr24). For introgression of PHST QTL, initially Lok1 was separately crossed with each of the two donors (PHS tolerant white-grained AUS1408 and CN19055). Backcrossing in each generation was followed by foreground and background selections using SSR markers. In advanced lines, KASP assay was also carried out for the candidate gene TaMKK3-A underlying the PHST QTL. The MAS derived lines homozygous for PHST QTL were screened for PHS using simulated rain chambers resulting in the selection of 10 PHST lines. For pyramiding of three QTL/genes (PHST QTL, Gpc-B1, and Lr24), MABB derived BC4F2 plants (from the cross Lok1/CN19055) were crossed with a MAS derived BC2F5 line [Lok1 (Gpc-B1 + Lr24)] developed earlier by us in the same background of Lok1. After foreground MAS followed by PHS screening, four advanced lines carrying all the three QTL/genes in homozygous condition were selected. These lines exhibited high level of PHST (PHS score 2–3) associated with significant improvement in GPC with no yield penalty and resistance against leaf rust under artificial epiphytotic conditions.

Injury potential of herbicide combinations on XtendFlex® cotton

XtendFlex® technology from Bayer allows growers to apply glyphosate, glufosinate, and dicamba POST to cotton. Since the evolution and spread of glyphosate-resistant weed species, early POST applications with several modes of action have become common. However, crop injury potential from these applications warrants further examination. Field studies were conducted from 2015 to 2017 at two locations in Mississippi to evaluate XtendFlex® cotton injury from herbicide application. Herbicide applications were made to XtendFlex® cotton at the 3 to 6 leaf stage with herbicide combinations comprised of two, three, and four-way combinations of glyphosate, glufosinate, S-metolachlor, and three formulations of dicamba. Data collection included visual estimations of injury, stand counts, cotton height, total mainstem nodes, and nodes above whiteflower at first bloom. Data collection at the end of the season included cotton height, total mainstem nodes, and nodes above cracked boll. Visual estimations of injury from herbicide applications were highest at 3 days following applications containing glufosinate + S-metolachlor (36 to 41% injury) and glufosinate + S-metolachlor in combination with dicamba + glyphosate (39 to 41% injury), regardless of the dicamba formulation. Crop injury decreased at each rating interval and dissipated by 28 days following applications (p = 0.3748). Height reductions were present at first bloom and at the end of the season (p < 0.0001), although cotton yield was unaffected (p = 0.2089) even when injury at 3 days after treatment (DAA) was greater than 30%. Results indicate that growers may apply a variety of herbicide tank-mixtures to XtendFlex® cotton and expect no yield penalty. Furthermore, if growers are concerned with cotton injury after herbicide applications, the use of glufosinate in combination with S-metolachlor should be approached with caution in XtendFlex® cotton.

Techno-Economic Viability of Agro-Photovoltaic Irrigated Arable Lands in the EU-Med Region: A Case-Study in Southwestern Spain

Solar photovoltaic (PV) energy is positioned to play a major role in the electricity generation mix of Mediterranean countries. Nonetheless, substantial increase in ground-mounted PV installed capacity could lead to competition with the agricultural use of land. A way to avert the peril is the electricity-food dual use of land or agro-photovoltaics (APV). Here, the profitability of a hypothetical APV system deployed on irrigated arable lands of southwestern Spain is analyzed. The basic generator design, comprised of fixed-tilt opaque monofacial PV modules on a 5 m ground-clearance substructure, featured 555.5 kWp/ha. Two APV shed orientations, due south and due southwest, were compared. Two 4-year annual-crop rotations, cultivated beneath the heightened PV modules and with each rotation spanning 24 ha, were studied. One crop rotation was headed by early potato, while the other was headed by processing tomato. All 9 crops involved fulfilled the two-fold condition of being usually cultivated in the area and compatible with APV shed intermitent shading. Crop revenues under the partial shading of PV modules were derived from official average yields in the area, through the use of two alternative sets of coefficients generated for low and high crop-yield shade-induced penalty. Likewise, two irrigation water sources, surface and underground, were compared. Crop total production costs, PV system investment and operating costs and revenues from the sale of electricity, were calculated. The internal rates of return (IRRs) obtained ranged from a minimum of 3.8% for the combination of southwest orientation, early-potato rotation, groundwater and high shade-induced crop-yield penalty, to a maximum of 5.6% for the combination of south orientation, processing-tomato rotation, surface water and low shade-induced crop-yield penalty.

Yield maintenance under drought is orchestrated by the qDTY12.1-encoded DECUSSATE gene of rice through a network with other flowering-associated genes across the genetic background

Introgression of major-effect QTLs is an important component of rice breeding for yield-retention under drought. While largely effective, the maximum potentials of such QTLs have not been consistent across genetic backgrounds. We hypothesized that synergism or antagonism with additive-effect peripheral genes across the background could either enhance or undermine the QTL effects. To elucidate the molecular underpinnings of such interaction, we dissected qDTY12.1 synergy with numerous peripheral genes in context of network rewiring effects. By integrative transcriptome profiling and network modeling, we identified the DECUSSATE (OsDEC) within qDTY12.1 as the core of the synergy and shared by two sibling introgression lines in IR64 genetic background, i.e., LPB (low-yield penalty) and HPB (high-yield penalty). OsDEC is expressed in flag leaves and induced by progressive drought at booting stage in LPB but not in HPB. The unique OsDEC signature in LPB is coordinated with 35 upstream and downstream peripheral genes involved in floral development through the cytokinin signaling pathway, which are lacking in HPB. Results further support the differential network rewiring effects through genetic coupling-uncoupling between qDTY12.1 and other upstream and downstream peripheral genes across the distinct genetic backgrounds of LPB and HPB. We propose that the functional DEC-network in LPB defines a mechanism for early flowering as a means for avoiding the depletion of photosyntate needed for reproductive growth due to drought. Its impact on yield-retention is likely through the timely establishment of stronger source-sink dynamics that sustains a robust reproductive transition under drought.Author summaryWhile the Green Revolution of the 1960’s significantly increased rice grain yields through the creation of high-yielding varieties for high input systems, current marginal climates pose a significant challenge for providing consistent yield. In rice growing regions of the world, drought affects the livelihood of small-scale and subsistence farmers by inflicting significant yield penalties to their production systems. Breeding of next-generation rice varieties with optimal balance of survivability and productivity traits will be key to providing consistent yields year to year. Within this paradigm, the use of large effect QTLs such as qDTY12.1 to improve yield retention under drought have been largely successful. By integrating the use of high resolution transcriptome datasets with a focused biological interrogation of agronomic results from this and previous studies, we uncovered a putative functional genetic network, anchored by the DECUSSATE gene (OsDEC) within qDTY12.1, that effectively minimizes drought penalties to yield by driving cellular processes that culminate in timely flowering that maximizes the use of photosynthetic sources for efficient reproduductive transition and ultimately seed development. Our study further illuminates the qDTY12.1 function and speaks to the misconception that qDTY introgression alone is sufficient for providing consistently large positive effects to yield retention under reproductive stage drought.