Combination of rice blast resistance genes in the genotypes of russian rice varieties with the use of marker assisted selection

Grain productivity of rice is significantly reduced by dangerous disease – blast. Therefore, the development of resistant high yielding rice varieties with the Pi group of genes is important. Use of molecular markers linked with the loci of resistance significantly optimizes the breeding process. The purpose of the research is to develop rice lines combining 2-6 loci of resistance to blast: Pi-1, Pi-2, Pi-33, Pi-ta, Pi-b, Pi-40 by molecular marking. Materials and Methods. As donors of resistance genes foreign samples were used, recipient – Russian varieties. In the studies we used micro-satellites markers and PCR analysis. Results. In the first stage of the research as a result of hybridization domestic lines with genes Pi-l, Pi-2, Pi-33 were obtained. At the second stage – hybrids with all 3 genes were developed. In the third stage genes Pi-b and Pi-ta and on the fourth – Pi-40 were introduced. Conclusion. As a result, rice genotypes, combining 6 loci of blast resistance were developed with use of marker assisted selection.

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Identification of novel rice blast resistance alleles through sequence-based allele mining

10.21203/rs.2.18859/v1 ◽

2019 ◽

Author(s):

Ying Zhou ◽

Fang Lei ◽

Qiong Wang ◽

Weicong He ◽

Yuan Bin ◽

...

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Blast Resistance ◽

Resistance Breeding ◽

Economic Losses ◽

The Novel ◽

Allele Mining ◽

Rice Varieties ◽

Rice Blast Resistance ◽

Blast Resistance Genes

Abstract Background: As rice ( Oryza sativa ) is the staple food of more than half the world’s population, rice production contributes greatly to global food security. Rice blast caused by the fungus M agnaporthe oryzae is a devastating fungal disease of rice, affecting yield and grain quality and resulting in substantial annual economic losses. Because the fungus evolves rapidly,, resistance conferred by most of the single blast race resistance genes is often broken after a few years of intensive agricultural use. Effective resistance breeding in rice therefore requires continual enrichment of the reservoir of resistance genes and alleles. Seed banks represent a rich source of genetic diversity; however, they have not been extensively used to identify novel genes and alleles. Results: We carried out a large-scale screen for novel blast resistance alleles in 1883 rice varieties from major rice producing areas across China. Of these, 107 varieties showed at least moderate resistance to natural infection by rice blast at rice blast nurseries in Enshi and Yichang, Hubei Province. Using sequence-based allele mining to amplify and clone the allelic variants of major rice blast resistance genes at the Pi2/9/gm/zt locus of chromosome 6 from the 107 blast-resistant varieties, we identified 13 novel blast resistance alleles. We then used controlled infections to assess the resistance of rice varieties carrying the novel alleles to 34 single rice blast isolates from Hubei, Guangdong, Jiangsu, Hunan, Jangxi, Sichuan, Heilongjiang, and Fujin Provinces. The varieties identified as being resistant in the nursery trials showed varied disease responses when infected with the single blast isolates, suggesting that the novel Pi2/9/gm/zt alleles vary in their blast resistance spectra. Some of the newly identified alleles have unique single nucleotide polymorphisms (SNPs), insertions, or deletions, in addition to polymorphic residues that are shared between the different alleles. Conclusions: These alleles expand the allelic series of blast resistance genes, enriching the genetic resource for rice blast resistance breeding programs and for studies aimed at deciphering rice–rice blast molecular interactions. Key words : Pi9 , R-genes, Nucleotide diversity, Gene conversion, Resistance gene alleles, Rice blast

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Expression-based genotyping of the rice blast resistance genes in the elite maintainer line Yixiang1B

European Journal of Plant Pathology ◽

10.1007/s10658-017-1149-1 ◽

2017 ◽

Vol 148 (4) ◽

pp. 955-965 ◽

Cited By ~ 1

Author(s):

Li Wang ◽

Xiao-Hong Hu ◽

Gang Lin ◽

De-Ming Zhao ◽

Jun Shi ◽

...

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Blast Resistance ◽

Maintainer Line ◽

Rice Blast Resistance ◽

Blast Resistance Genes

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Screening of the Dominant Rice Blast Resistance Genes with PCR-based SNP and CAPS Marker in Aromatic Rice Germplasm

Korean Journal of Crop Science ◽

10.7740/kjcs.2011.56.4.329 ◽

2011 ◽

Vol 56 (4) ◽

pp. 329-341 ◽

Cited By ~ 1

Author(s):

Jeong-Soon Kim ◽

Sang-Nag Ahn ◽

Sung-Jun Hong ◽

Jin-Hyeuk Kwon ◽

Yeong-Ki Kim ◽

...

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Blast Resistance ◽

Aromatic Rice ◽

Caps Marker ◽

Rice Blast Resistance ◽

Rice Germplasm ◽

Blast Resistance Genes

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Identification of rice blast (Pyricularia) resistance genes in the breeding samples of rice

E3S Web of Conferences ◽

10.1051/e3sconf/202017501008 ◽

2020 ◽

Vol 175 ◽

pp. 01008

Author(s):

Nataliya Vozhzhova ◽

Elena Krasnova ◽

Elena Ionova

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Agricultural Research ◽

Blast Resistance ◽

Genetic Material ◽

Climatic Conditions ◽

Rice Blast Resistance ◽

Rice Samples ◽

Functional Alleles ◽

Blast Resistance Genes

The current paper has considered the challenge of breeding of rice lines adapted to the climatic conditions of the Southern Federal District of Russia by pyramiding a complex of Pi-1, Pi-2, Pi-33, Pi-ta and Pi-b rice blast resistance genes which is efficient in the southern part of Russia. The control of the resistance genes present in the breeding material was carried out using a marker-associated selection (MAS). The purpose of the current study was to identify the Pi-1, Pi-2, Pi-33, Pi-ta, and Pi-b rice blast resistance genes in the rice samples developed in the laboratory of rice breeding and seed production of the FSBSI “Agricultural Research Center “Donskoy” to identify valuable genotypes. The screening of 689 breeding samples of rice resulted in identification of the allelic state of the Pi-1, Pi-2, Pi-33, Pi-ta and Pi-b rice blast resistance genes, and the genotypes with functional alleles of these genes and their combinations. There were identified 19 breeding samples of rice possessing a combination of 4 functional alleles of rice blast resistance genes in various combinations. The identified rice samples are valuable genetic material and are recommended to use as the sources of rice blast resistance in the breeding process.

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Rice Breeding in Russia Using Genetic Markers

Plants ◽

10.3390/plants9111580 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1580

Author(s):

Elena Dubina ◽

Pavel Kostylev ◽

Margarita Ruban ◽

Sergey Lesnyak ◽

Elena Krasnova ◽

...

Keyword(s):

Resistance Genes ◽

Target Genes ◽

Blast Resistance ◽

Soil Salinization ◽

Water Flooding ◽

Rice Varieties ◽

Asian Rice ◽

Southern Russia ◽

Tolerance Gene ◽

Blast Resistance Genes

The article concentrates on studying tolerance to soil salinization, water flooding, and blast in Russian and Asian rice varieties, as well as hybrids of the second and third generations from their crossing in order to obtain sustainable paddy crops based on domestic varieties using DNA markers. Samples IR 52713-2B-8-2B-1-2, IR 74099-3R-3-3, and NSIC Rc 106 were used as donors of the SalTol tolerance gene. Varieties with the Sub1A locus were used as donors of the flood resistance gene: Br-11, CR-1009, Inbara-3, TDK-1, and Khan Dan. The lines C101-A-51 (Pi-2), C101-Lac (Pi-1, Pi-33), IR-58 (Pi-ta), and Moroberekan (Pi-b) were used to transfer blast resistance genes. Hybridization of the stress-sensitive domestic varieties Novator, Flagman, Virazh, and Boyarin with donor lines of the genes of interest was carried out. As a result of the studies carried out using molecular marking based on PCR in combination with traditional breeding, early-maturing rice lines with genes for resistance to salinity (SalTol) and flooding (Sub1A), suitable for cultivation in southern Russia, were obtained. Introgression and pyramiding of the blast resistance genes Pi-1, Pi-2, Pi-33, Pi-ta, and Pi-b into the genotypes of domestic rice varieties were carried out. DNA marker analysis revealed disease-resistant rice samples carrying 5 target genes in a homozygous state. The created rice varieties that carry the genes for blast resistance (Pentagen, Magnate, Pirouette, Argamac, Kapitan, and Lenaris) were submitted for state variety testing. The introduction of such varieties into production will allow us to avoid epiphytotic development of the disease, preserving the biological productivity of rice and obtaining environmentally friendly agricultural products.

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