scholarly journals Arbuscular mycorrhiza Symbiosis Induces a Major Transcriptional Reprogramming of the Potato SWEET Sugar Transporter Family

2016 ◽  
Vol 7 ◽  
Author(s):  
Jasmin Manck-Götzenberger ◽  
Natalia Requena
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Sugar Transporter ◽  
Transcriptional Reprogramming ◽  
Transporter Family

scholarly journals The HXT2 gene of Saccharomyces cerevisiae is required for high-affinity glucose transport.

1990 ◽  
Vol 10 (11) ◽  
pp. 5903-5913 ◽  
Author(s):  
A L Kruckeberg ◽  
L F Bisson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glucose Transport ◽  
Large Family ◽  
Transmembrane Domains ◽  
Growth Defect ◽  
Multicopy Plasmid ◽  
Sugar Transporter ◽  
Yeast Saccharomyces Cerevisiae ◽  
High Affinity ◽  
Transporter Family

The HXT2 gene of the yeast Saccharomyces cerevisiae was identified on the basis of its ability to complement the defect in glucose transport of a snf3 mutant when present on the multicopy plasmid pSC2. Analysis of the DNA sequence of HXT2 revealed an open reading frame of 541 codons, capable of encoding a protein of Mr 59,840. The predicted protein displayed high sequence and structural homology to a large family of procaryotic and eucaryotic sugar transporters. These proteins have 12 highly hydrophobic regions that could form transmembrane domains; the spacing of these putative transmembrane domains is also highly conserved. Several amino acid motifs characteristic of this sugar transporter family are also present in the HXT2 protein. An hxt2 null mutant strain lacked a significant component of high-affinity glucose transport when under derepressing (low-glucose) conditions. However, the hxt2 null mutation did not incur a major growth defect on glucose-containing media. Genetic and biochemical analyses suggest that wild-type levels of high-affinity glucose transport require the products of both the HXT2 and SNF3 genes; these genes are not linked. Low-stringency Southern blot analysis revealed a number of other sequences that cross-hybridize with HXT2, suggesting that S. cerevisiae possesses a large family of sugar transporter genes.

The nucleotide-sugar transporter family: a phylogenetic approach

Biochimie ◽  
2003 ◽  
Vol 85 (3-4) ◽  
pp. 245-260 ◽  
Author(s):  
Ivan Martinez-Duncker ◽  
Rosella Mollicone ◽  
Patrice Codogno ◽  
Rafael Oriol
Keyword(s):  
Sugar Transporter ◽  
Nucleotide Sugar ◽  
Transporter Family ◽  
Nucleotide Sugar Transporter

scholarly journals Comprehensive Analysis of Evolutionary, Characterization and Expression for Sugar Transporter Family Genes in Nelumbo nucifera 

2020 ◽  
Author(s):  
Peng Wu ◽  
Yongyan Zhang ◽  
Liangjun Li
Keyword(s):  
Large Scale ◽  
Comprehensive Analysis ◽  
Nelumbo Nucifera ◽  
Scale Analysis ◽  
Sugar Transporter ◽  
Chain Reaction ◽  
Evolutionary Trajectory ◽  
Large Scale Analysis ◽  
Transporter Family ◽  
Polymerase Chain

Abstract BackgroundThe sugar transporter , an important class of transporter for sugars function, play regulators of many processes associated with growth, maturation and senescence processes in plant.ResultsIn this study, a total of 35NNUSTs were identified in the Nelumbo nucifera genome. Furthermore, all NNUSTs genes identified were grouped according to conserved domains and phylogenetic analysis. Additionally, we identified 316 ST genes in other10 representative plants, and performed a comparative analysis with Nelumbo nucifera, including evolutionary trajectory, gene duplication, and expression pattern. A large-scale analysis across plants and alga suggested that the ST family could have been originated from STP and Glct, expanding to form STP and SFP by dispersed duplication. Finally, quantitative real-time polymerase chain reaction and cis-elements analysis showed that the NNUSTs were response to abiotic stresses. ConclusionsThis provides useful resources when exploring the molecular evolution and mechanisms of NNUSTs in plants.

scholarly journals A Member of the Sugar Transporter Family, Stl1p Is the Glycerol/H+Symporter inSaccharomyces cerevisiae

2005 ◽  
Vol 16 (4) ◽  
pp. 2068-2076 ◽  
Author(s):  
Célia Ferreira ◽  
Frank van Voorst ◽  
António Martins ◽  
Luisa Neves ◽  
Rui Oliveira ◽  
...  
Keyword(s):  
Osmotic Shock ◽  
Proton Motive Force ◽  
Dependent Manner ◽  
Sugar Transporter ◽  
Transporter Family ◽  
Glycerol Transport ◽  
Proton Symport ◽  
Genes Encoding ◽  
Glycerol Uptake ◽  
Mutant Collection

Glycerol and other polyols are used as osmoprotectants by many organisms. Several yeasts and other fungi can take up glycerol by proton symport. To identify genes involved in active glycerol uptake in Saccharomyces cerevisiae we screened a deletion mutant collection comprising 321 genes encoding proteins with 6 or more predicted transmembrane domains for impaired growth on glycerol medium. Deletion of STL1, which encodes a member of the sugar transporter family, eliminates active glycerol transport. Stl1p is present in the plasma membrane in S. cerevisiae during conditions where glycerol symport is functional. Both the Stl1 protein and the active glycerol transport are subject to glucose-induced inactivation, following identical patterns. Furthermore, the Stl1 protein and the glycerol symporter activity are strongly but transiently induced when cells are subjected to osmotic shock. STL1 was heterologously expressed in Schizosaccharomyces pombe, a yeast that does not contain its own active glycerol transport system. In S. pombe, STL1 conferred the ability to take up glycerol against a concentration gradient in a proton motive force-dependent manner. We conclude that the glycerol proton symporter in S. cerevisiae is encoded by STL1.

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