scholarly journals Population Structure and Phylogenetic Relationships in a Diverse Panel of Brassica rapa L.

2017 ◽  
Vol 8 ◽  
Author(s):  
Kevin A. Bird ◽  
Hong An ◽  
Elodie Gazave ◽  
Michael A. Gore ◽  
J. Chris Pires ◽  
...  
Author(s):  
R. H. Sammour ◽  
M. A. Karam ◽  
Y. S. Morsi ◽  
R. M. Ali

Abstract The present study aimed to assess population structure and phylogenetic relationships of nine subspecies of Brassica rapa L. represented with thirty-five accessions cover a wide range of species distribution area using isozyme analysis in order to select more diverse accessions as supplementary resources that can be utilized for improvement of B. napus. Enzyme analysis resulted in detecting 14 putative polymorphic loci with 27 alleles. Mean allele frequency 0.04 (rare alleles) was observed in Cat4A and Cat4B in sub species Oleifera accession CR 2204/79 and in subspecies trilocularis accessions CR 2215/88 and CR 2244/88. The highest genetic diversity measures were observed in subspecies dichotoma, accession CR 1585/96 (the highest average of observed (H0) and expected heterozygosity (He), and number of alleles per locus (Ae)). These observations make this accession valuable genetic resource to be included in breeding programs for the improvement of oilseed B. napus. The average fixation index (F) is significantly higher than zero for the analysis accessions indicating a significant deficiency of heteozygosity. The divergence among subspecies indicated very great genetic differentiation (FST = 0.8972) which means that about 90% of genetic diversity is distributed among subspecies, while 10% of the diversity is distributed within subspecies. This coincides with low value of gene flow (Nm = 0.0287). B. rapa ssp. oleifera (turnip rape) and B. rapa ssp. trilocularis (sarson) were grouped under one cluster which coincides with the morphological classification.


2014 ◽  
Vol 63 (6) ◽  
pp. 1395-1403 ◽  
Author(s):  
C.-H. Lin ◽  
K.-C. Tsai ◽  
P. Prior ◽  
J.-F. Wang

2015 ◽  
Vol 35 (1) ◽  
Author(s):  
Wenxing Pang ◽  
Xiaonan Li ◽  
Su Ryun Choi ◽  
Vignesh Dhandapani ◽  
Subin Im ◽  
...  

Genome ◽  
2007 ◽  
Vol 50 (10) ◽  
pp. 963-973 ◽  
Author(s):  
Jianjun Zhao ◽  
Maria-João Paulo ◽  
Diaan Jamar ◽  
Ping Lou ◽  
Fred van Eeuwijk ◽  
...  

Association mapping was used to investigate the genetic basis of variation within Brassica rapa , which is an important vegetable and oil crop. We analyzed the variation of phytate and phosphate levels in seeds and leaves and additional developmental and morphological traits in a set of diverse B. rapa accessions and tested association of these traits with AFLP markers. The analysis of population structure revealed four subgroups in the population. Trait values differed between these subgroups, thus defining associations between population structure and trait values, even for traits such as phytate and phosphate levels. Marker–trait associations were investigated both with and without taking population structure into account. One hundred and seventy markers were found to be associated with the observed traits without correction for population structure. Association analysis with correction for population structure led to the identification of 27 markers, 6 of which had known map positions; 3 of these were confirmed in additional QTL mapping studies.


2017 ◽  
Vol 108 (1) ◽  
pp. 5-13 ◽  
Author(s):  
J.M. Wainaina ◽  
P. De Barro ◽  
L. Kubatko ◽  
M. A. Kehoe ◽  
J. Harvey ◽  
...  

AbstractTrialeurodes vaporariorum (Westwood, 1856) (Greenhouse whitefly) is an agricultural pest of global importance. It is associated with damage to plants during feeding and subsequent virus transmission. Yet, global phylogenetic relationships, population structure, and estimation of the rates of gene flow within this whitefly species remain largely unexplored. In this study, we obtained and filtered 227 GenBank records of mitochondrial cytochrome c oxidase I (mtCOI) sequences of T. vaporariorum, across various global locations to obtain a final set of 217 GenBank records. We further amplified and sequenced a ~750 bp fragment of mtCOI from an additional 31 samples collected from Kenya in 2014. Based on a total of 248 mtCOI sequences, we identified 16 haplotypes, with extensive overlap across all countries. Population structure analysis did not suggest population differentiation. Phylogenetic analysis indicated the 2014 Kenyan collection of samples clustered with a single sequence from the Netherlands to form a well-supported clade (denoted clade 1a) nested within the total set of sequences (denoted clade 1). Pairwise distances between sequences show greater sequence divergence between clades than within clades. In addition, analysis using migrate-n gave evidence for recent gene flow between the two groups. Overall, we find that T. vaporariorum forms a single large group, with evidence of further diversification consisting primarily of Kenyan sequences and one sequence from the Netherlands forming a well-supported clade.


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