Modification of Barley Plant Productivity Through Regulation of Cytokinin Content by Reverse-Genetics Approaches

Plants have evolved the ability to derive the benzenoid moiety of the respiratory cofactor and antioxidant, ubiquinone (coenzyme Q), either from the β-oxidative metabolism of p-coumarate or from the peroxidative cleavage of kaempferol. Here, isotopic feeding assays, gene co-expression analysis and reverse genetics identified Arabidopsis 4-COUMARATE-COA LIGASE 8 (4-CL8; At5g38120) as a contributor to the β-oxidation of p-coumarate for ubiquinone biosynthesis. The enzyme is part of the same clade (V) of acyl-activating enzymes than At4g19010, a p-coumarate CoA ligase known to play a central role in the conversion of p-coumarate into 4-hydroxybenzoate. A 4-cl8 T-DNA knockout displayed a 20% decrease in ubiquinone content compared with wild-type plants, while 4-CL8 overexpression boosted ubiquinone content up to 150% of the control level. Similarly, the isotopic enrichment of ubiquinone's ring was decreased by 28% in the 4-cl8 knockout as compared with wild-type controls when Phe-[Ring-13C6] was fed to the plants. This metabolic blockage could be bypassed via the exogenous supply of 4-hydroxybenzoate, the product of p-coumarate β-oxidation. Arabidopsis 4-CL8 displays a canonical peroxisomal targeting sequence type 1, and confocal microscopy experiments using fused fluorescent reporters demonstrated that this enzyme is imported into peroxisomes. Time course feeding assays using Phe-[Ring-13C6] in a series of Arabidopsis single and double knockouts blocked in the β-oxidative metabolism of p-coumarate (4-cl8; at4g19010; at4g19010 × 4-cl8), flavonol biosynthesis (flavanone-3-hydroxylase), or both (at4g19010 × flavanone-3-hydroxylase) indicated that continuous high light treatments (500 µE m−2 s−1; 24 h) markedly stimulated the de novo biosynthesis of ubiquinone independently of kaempferol catabolism.

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Characterization of spelt collection samples (Triticum spelta L.) by elements of plant productivity structure and baking quality

Fiziologia rastenij i genetika ◽

10.15407/frg2016.02.112 ◽

2016 ◽

Vol 48 (2) ◽

pp. 112-119 ◽

Cited By ~ 4

Author(s):

V.V. Morgun ◽

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S.M. Sichkar ◽

V.M. Pochinok ◽

A.K. Ninieva ◽

...

Keyword(s):

Plant Productivity ◽

Triticum Spelta ◽

Baking Quality

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CORRELATION RELATIONS BETWEEN VALUABLE IRRIGATED SOIL PROPERTIES AND PLANT PRODUCTIVITY

JOURNAL OF AGRO PROCESSING ◽

10.26739/2181-9904-2020-5-7 ◽

2020 ◽

Vol 5 (2) ◽

pp. 37-42

Author(s):

Inobat Ruzieva ◽

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Inobat Ruzieva ◽

Islom Xaitov ◽

Ulug`berdi Xursanov

Keyword(s):

Soil Properties ◽

Plant Productivity

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ANALISIS KESESUAIAN LAHAN DAN EVALUASI JENIS TANAH DALAM BUDIDAYA TANAMAN TEBU UNTUK PENGEMBANGAN DAERAH KABUPATEN TEGAL

Jurnal Sains dan Teknologi Indonesia ◽

10.29122/jsti.v13i2.878 ◽

2013 ◽

Vol 13 (2) ◽

Author(s):

Daru Mulyono

Keyword(s):

Remote Sensing ◽

Information System ◽

Spatial Analysis ◽

Geographical Information System ◽

Plant Productivity ◽

Saccharum Officinarum ◽

Geographical Information ◽

Sugarcane Plant ◽

Plant Cultivation ◽

Suitability Map

The objectives of the research were to make land suitability map for sugarcane plant (Saccharum officinarum), to give recommendation of location including area for sugarcane plant cultivation and to increase sugarcane plant productivity. The research used maps overlay and Geographical Information System (GIS) which used Arch-View Spatial Analysis version 2,0 A in Remote Sensing Laboratory, Agency for the Assessment and Application of Technology (BPPT), Jakarta. The research was carried out in Tegal Regency starting from June to October 2004.The results of the research showed that the suitable, conditionally suitable, and not suitable land for sugarcane cultivation in Tegal Regency reached to a high of 20,227 ha, 144 ha, and 81,599 ha respectively. There were six most dominant kind of soil: alluvial (32,735 ha), grumosol 5,760 ha), mediteran (17,067 ha), latosol (18,595 ha), glei humus (596 ha), and regosol (22,721 ha).

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