Overexpression of Arabidopsis Nucleotide-Binding and Leucine-Rich Repeat Genes RPS2 and RPM1(D505V) Confers Broad-Spectrum Disease Resistance in Rice

Rice blast caused by Magnaporthe oryzae is the most destructive fungal disease in crops, greatly threatening rice production and food security worldwide. The identification and utilization of broad-spectrum resistance genes are considered to be the most economic and effective method to control the disease. In the past decade, many blast resistance ( R ) genes have been identified, which mainly encode nucleotide-binding leucine-rich repeat (NLR) receptor family and confer limited race-specific resistance to the fungal pathogen. Resistance genes conferring broad-spectrum blast resistance are still largely lacking. In this study, we carried out a map-based cloning of the new blast R locus Pizh in variety ZH11. A bacterial artificial chromosome (BAC) clone of 165 kb spanning the Pizh locus was sequenced and identified 9 NLR genes, among which only Pizh-1 and Pizh-2 were expressed. Genetic complementation experiments indicated that Pizh-1 but not Pizh-2 alone could confer blast resistance. Intriguingly, both mutations on Pizh-1 and Pizh-2 by CRISPR-Cas9 abolished the Pizh- mediated resistance. We also observed that Pizh-1 -mediated resistance was partially dependent on Pizh-2 . Pizh-1 and Pizh-2 form a complex of NLRs through direct interaction. This suggests that Pizh-1 may function as the executor NLR and Pizh-2 as a ‘helper’ NLR that shares functional redundancy with other NLRs. Our current study provides not only a good tool for rice disease resistance breeding but also deep insight into NLR association and function in plant immunity. This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.

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Genome- Wide Analysis of the Nucleotide Binding Site Leucine-Rich Repeat Genes of Four Orchids Revealed Extremely Low Numbers of Disease Resistance Genes

Frontiers in Genetics ◽

10.3389/fgene.2019.01286 ◽

2020 ◽

Vol 10 ◽

Cited By ~ 4

Author(s):

Jia-Yu Xue ◽

Tao Zhao ◽

Yang Liu ◽

Yong-Xia Zhang ◽

...

Keyword(s):

Disease Resistance ◽

Binding Site ◽

Resistance Genes ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Disease Resistance Genes ◽

Leucine Rich Repeat ◽

Genome Wide Analysis ◽

Genome Wide

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The Broad-Spectrum Blast Resistance GenePi9Encodes a Nucleotide-Binding Site–Leucine-Rich Repeat Protein and Is a Member of a Multigene Family in Rice

Genetics ◽

10.1534/genetics.105.044891 ◽

2005 ◽

Vol 172 (3) ◽

pp. 1901-1914 ◽

Cited By ~ 339

Author(s):

Shaohong Qu ◽

Guifu Liu ◽

Bo Zhou ◽

Maria Bellizzi ◽

Lirong Zeng ◽

...

Keyword(s):

Binding Site ◽

Broad Spectrum ◽

Multigene Family ◽

Blast Resistance ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Leucine Rich Repeat ◽

Repeat Protein ◽

Leucine Rich Repeat Protein

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Map-based cloning of a gene sequence encoding a nucleotide-binding domain and a leucine-rich region at the Cre3 nematode resistance locus of wheat

Genome ◽

10.1139/g97-087 ◽

1997 ◽

Vol 40 (5) ◽

pp. 659-665 ◽

Cited By ~ 87

Author(s):

Evans S. Lagudah ◽

Odile Moullet ◽

Rudi Appels

Keyword(s):

Disease Resistance ◽

Gene Family ◽

Binding Site ◽

Resistance Gene ◽

Resistance Genes ◽

Gene Sequence ◽

Nucleotide Binding ◽

Disease Resistance Gene ◽

Leucine Rich Repeat ◽

Rich Region

The Cre3 gene confers a high level of resistance to the root endoparasitic nematode Heterodera avenae in wheat. A DNA marker cosegregating with H. avenae resistance was used as an entry point for map-based cloning of a disease resistance gene family at the Cre3 locus. Two related gene sequences have been analysed at the Cre3 locus. One, identified as a cDNA clone, encodes a polypeptide with a nucleotide binding site (NBS) and a leucine-rich region; this member of the disease resistance gene family is expressed in roots. A second Cre3 gene sequence, cloned as genomic DNA, appears to be a pseudogene, with a frame shift caused by a deletion event. These two genes, related to members of the cytoplasmic NBS – leucine rich repeat class of plant disease resistance genes were physically mapped to the distal 0.06 fragment of the long arm of wheat chromosome 2D and cosegregated with nematode resistance.Key words: cereal cyst nematode, disease resistance genes, nucleotide-binding site, leucine-rich repeat.

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A superfamily of disease resistance gene analogs is located on all homoeologous chromosome groups of wheat (Triticum aestivum)

Genome ◽

10.1139/g98-083 ◽

1998 ◽

Vol 41 (6) ◽

pp. 782-788 ◽

Cited By ~ 11

Author(s):

W Spielmeyer ◽

M Robertson ◽

N Collins ◽

D Leister ◽

P Schulze-Lefert ◽

...

Keyword(s):

Disease Resistance ◽

Binding Site ◽

Resistance Gene ◽

Resistance Genes ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Resistance Gene Analogs ◽

Leucine Rich Repeat ◽

Homoeologous Chromosome ◽

Entry Points

In this study, resistance gene analogs (RGAs) which were isolated from monocot crop species (wheat, barley, maize and rice) and contained conserved sequence motifs found within the nucleotide binding site - leucine rich repeat (NBS-LRR) class of resistance genes, were used to assess their distribution in the wheat genome. The RGAs showed 30-70% amino acid identity to a previously isolated monocot NBS-LRR sequence from the Cre3 locus for cereal cyst nematode (CCN) resistance in wheat. We used the RGAs as probes to identify and map loci in wheat using recombinant inbred lines of an international Triticeae mapping family. RGA loci mapped across all seven homoeologous chromosome groups of wheat. This study demonstrated that the RGA mapping approach provides potential entry points toward identifying resistance gene candidates in wheat.Key words: wheat, disease resistance genes, nucleotide binding site, leucine rich repeat, resistance gene analogs.

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