scholarly journals Genomic Rearrangement and Recombination of Porcine Circovirus Type 2 and Porcine Circovirus-Like Virus P1 in China

2021 ◽  
Vol 8 ◽  
Author(s):  
Libin Wen ◽  
Kongwang He
Keyword(s):  
Genome Rearrangement ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Protein Coding ◽  
Generic Term

Porcine circovirus type 2 (PCV2) belongs to the genus Circovirus of the family Circoviridae, and it has been associated with porcine circovirus (associated) disease (PCVD or PCVAD) in pigs. PCVAD is the generic term for a series of disease syndromes that have caused economic losses to the pig industry worldwide. Since the discovery of PCV2 in the late 1990s, the virus has continued to evolve, and novel genotypes have continued to appear. Moreover, there has been recombination between different genotypes of PCV2. This review attempts to illustrate some progress concerning PCV2 in genome rearrangement and genomic recombination with non-PCV2-related nucleic acids, particularly focusing on the porcine circovirus-like virus P1 formed by the recombination of PCV2. The presence of rearranged PCV2 genomes can be demonstrated both in vivo and in vitro, and these subviral molecules ranged from 358 to 1,136 bp. Depending on whether it has the ability to encode a protein, the agents formed by PCV2 recombination can be divided into two categories: porcine circovirus-like viruses and porcine circovirus-like mini agents. We mainly discuss the porcine circovirus-like virus P1 regarding genomic characterization, etiology, epidemiology, and pathogenesis. Further research needs to be conducted on the pathogenicity of other porcine circovirus-like viruses and porcine circovirus-like mini agents and the effects of their interactions with PCV2, especially for the porcine circovirus-like mini agents that do not have protein-coding functions in the genome.

scholarly journals Ochratoxin A-induced autophagy in vitro and in vivo promotes porcine circovirus type 2 replication

2017 ◽  
Vol 8 (6) ◽  
pp. e2909-e2909 ◽  
Author(s):  
Gang Qian ◽  
Dandan Liu ◽  
Junfa Hu ◽  
Fang Gan ◽  
Lili Hou ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus

A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) is genetically stable in vitro and in vivo

Vaccine ◽  
2008 ◽  
Vol 26 (33) ◽  
pp. 4231-4236 ◽  
Author(s):  
J. Gillespie ◽  
N.M. Juhan ◽  
J. DiCristina ◽  
K.F. Key ◽  
S. Ramamoorthy ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Genetically Engineered ◽  
Porcine Circovirus ◽  
Chimeric Vaccine

scholarly journals Increased porcine circovirus type 2 replication in porcine leukocytes in vitro and in vivo by concanavalin A stimulation

2008 ◽  
Vol 132 (1-2) ◽  
pp. 74-86 ◽  
Author(s):  
D.J. Lefebvre ◽  
P. Meerts ◽  
S. Costers ◽  
G. Misinzo ◽  
F. Barbé ◽  
...  
Keyword(s):  
Concanavalin A ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus

scholarly journals Ochratoxin A promotes porcine circovirus type 2 replication in vitro and in vivo

2015 ◽  
Vol 80 ◽  
pp. 33-47 ◽  
Author(s):  
Fang Gan ◽  
Zheqian Zhang ◽  
Zhihua Hu ◽  
John Hesketh ◽  
Hongxia Xue ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus

scholarly journals In vitro and in vivo characterization of an infectious clone of a European strain of porcine circovirus type 2

2004 ◽  
Vol 85 (5) ◽  
pp. 1259-1266 ◽  
Author(s):  
M. Roca ◽  
M. Balasch ◽  
J. Segalés ◽  
M. Calsamiglia ◽  
E. Viaplana ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Infectious Clone ◽  
Experimental Period ◽  
Porcine Circovirus Type ◽  
Virus Genome ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus

The aim of this study was to describe the generation of a PCV2 (porcine circovirus type 2) infectious clone (pIC-PCV2) and its infectivity under in vitro and in vivo conditions. The constructed pIC-PCV2 contained the whole PCV2 genome from a German isolate together with a partial duplication of 467 bp. PK-15 cells were transfected with pIC-PCV2 and an indirect immune fluorescence assay (IFA) was performed 7 days post-transfection. The PCV2 Cap gene was expressed in approximately 20 % of the cultured cells, and only the recombination product, and not pIC-PCV2, was subsequently detected by PCR and Southern blot. This result indicated that infection by pIC-PCV2 delivered genomic PCV2 DNA specifically into susceptible cells and led to the expression of a functional virus genome. Eighteen 30- to 40-day-old conventional pigs were distributed into three groups. Group 1 pigs (n=6) were inoculated intranasally (i.n.) with a Spanish isolate of PCV2 propagated in cell culture; pigs from group 2 (n=6) were inoculated with pIC-PCV2 intramuscularly (i.m.), and the last group of pigs (n=6) was inoculated with pIC-PCV2 intraperitoneally (i.p.). All pigs remained clinically healthy during the whole experimental period (35 days). Pigs that received pIC-PCV2 i.p. and i.m., as well as those PCV2 i.n. inoculated, became infected based on an in situ hybridization (ISH), PCR, TaqMan PCR and serological results. The results of this study confirm that cloned PCV2 genomic DNA is infectious both in vitro and in vivo, and is able to cause PMWS-like lesions in i.p. and i.m. experimentally inoculated pigs.

scholarly journals Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins

Viruses ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 544
Author(s):  
Ting Ouyang ◽  
Guyu Niu ◽  
Yifang Zhang ◽  
Xiaohua Liu ◽  
Xinwei Zhang ◽  
...  
Keyword(s):  
Early Stage ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Pcv2 Infection ◽  
Porcine Circovirus ◽  
Protein Phosphatase 2 ◽  
Amp Activated Protein Kinase

Porcine circovirus type 2 (PCV2) is the etiological agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVDs/PCVADs). However, the pathogenesis of PCV2 is not fully understood. We previously found that 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is negatively associated with PCV2 infection in vitro and in vivo. HMGCR inhibits the early stages of PCV2 infection, while PCV2 infection induces the phosphorylation of HMGCR to inactivate the protein. In this study, we investigated the possibility that adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK), and protein phosphatase 2 (PP2A) participate in HMGCR-mediated inhibition of PCV2 infection and the interaction of porcine HMGCR with PCV2 proteins. The results showed that AMPK activity fluctuated in cells during the early stage of PCV2 infection, while PP2A had little effect on PCV2 infection and HMGCR activity. Furthermore, PCV2 infection may enhance or maintain the level of phosphorylated HMGCR by directly interacting with the protein in PK-15 cells. These findings may provide a better understanding of PCV2 pathogenesis, and HMGCR may be a novel PCV2 antiviral target.

scholarly journals Effect of deoxynivalenol (DON) mycotoxin on in vivo and in vitro porcine circovirus type 2 infections

2015 ◽  
Vol 176 (3-4) ◽  
pp. 257-267 ◽  
Author(s):  
Christian Savard ◽  
Chantale Provost ◽  
Fernando Alvarez ◽  
Vicente Pinilla ◽  
Nedzad Music ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus
Sign in / Sign up

Export Citation Format

Share Document