scholarly journals UV-Vis Spectrophotometric Analysis of DNA Retrieval for DNA Storage Applications

Actuators ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 246
Author(s):  
Aamir Minhas-Khan ◽  
Morteza Ghafar-Zadeh ◽  
Tina Shaffaf ◽  
Saghi Forouhi ◽  
Anthony Scime ◽  
...  
Keyword(s):  
Storage Time ◽  
Spectrophotometric Analysis ◽  
Retrieval Process ◽  
Term Retention ◽  
Time Period ◽  
Dna Storage ◽  
Long Term Retention ◽  
Hydrophilicity And Hydrophobicity ◽  
The Relationship

Informational Deoxyribonucleic Acid (iDNA) has gained the attention of many researchers and pioneer companies for the development of novel storage systems for the long-term and high-density storing of information. This research focuses on the physical storage of iDNA strands to address some of the current challenges by evaluating the accuracy of the process of iDNA retrieval from the surface after the dehydration process. For this aim, a UV-Vis spectrophotometric technique was used to measure the concentration of the DNA samples. Although spectroscopy has been widely employed for the evaluation of DNA concentration and contamination in a solution, it has not been used to investigate dry-state DNA, which is one of the preferred storage formats for the long-term retention of information. These results demonstrate that the UV-Vis spectrophotometric technique can be used to accurately measure dry-state DNA before the retrieval and its residues after the DNA retrieval process. This paper further examines the storage/retrieval process by investigating the relationship between the storage time and the amount of retrieved DNA or the DNA residue left on various surfaces. Based on the experimental results demonstrated and discussed in this paper, UV-Vis spectrophotometry can be used for monitoring dry-state DNA with a high accuracy larger than 98%. Moreover, these results reveal that the hydrophilicity and hydrophobicity of the surface do not significantly affect DNA retrieval over a one-month time period.

Iterative processing of information during sleep may improve consolidation

2000 ◽  
Vol 23 (6) ◽  
pp. 919-919 ◽  
Author(s):  
Carlo Cipolli
Keyword(s):  
Dream Recall ◽  
Positive Role ◽  
Iterative Processing ◽  
Term Retention ◽  
Long Term Retention ◽  
The 1950S ◽  
The Relationship

The relationship between sleep and memory has been controversial since the 1950s. Studies on delayed dream recall and long-term retention of pre-sleep stimuli indicate that sleep may have a positive role in the consolidation of information. This positive indication counterbalances the negative one from the studies on the effects of REM deprivation.[Vertes & Eastman]

Forgetting as a Function of Sleep at Different Times of Day

1972 ◽  
Vol 24 (4) ◽  
pp. 386-393 ◽  
Author(s):  
G. R. J. Hockey ◽  
S. Davies ◽  
M. M. Gray
Keyword(s):  
Retention Interval ◽  
Sleep Stages ◽  
Free Recall Task ◽  
Immediate Recall ◽  
Term Retention ◽  
Time Period ◽  
Long Term Retention ◽  
Function Of Sleep ◽  
Marked Drop

The experiment studied the separate effects of sleep and time period of retention interval on forgetting. A free recall task was given to independent groups of subjects either at night or in the morning, and a second recall demanded 5 h later, after an intervening period of sleeping or waking activity. Oral body temperatures (BT) were measured at each session. The data were analysed in terms of (a) immediate recall at test 1, and (b) amount forgotten from test 1 to test 2. Immediate recall was higher for morning groups, in agreement with previous findings, serial position analysis indicating that the effect is confined to enhancement of the primary memory component. Long-term retention was higher over the night interval, irrespective of sleeping conditions, though having slept at night produced better retention than having stayed awake. Sleep during the morning was not effective in reducing forgetting. BT showed a marked drop for both night groups and rise for day groups over the retention interval. Alternative explanations for the classical sleep/memory findings are suggested in terms of (a) differential effects of sleep stages on memory, and (b) the underlying diurnal variation in BT and other processes.

Long-term retention is greater following closed-book tests than open-book tests

2006 ◽  
Author(s):  
Pooja K. Agarwal ◽  
Jeffrey D. Karpicke ◽  
Sean H. Kang ◽  
Henry L. Roediger ◽  
Kathleen B. McDermott
Keyword(s):  
Open Book ◽  
Term Retention ◽  
Long Term Retention

Retrieval practice promotes long-term retention irrespective of the placement

2020 ◽  
Author(s):  
alice latimier ◽  
Arnaud Rierget ◽  
Son Thierry Ly ◽  
Franck Ramus
Keyword(s):  
Retention Interval ◽  
Retrieval Practice ◽  
Memory Retention ◽  
Term Retention ◽  
Long Term Retention

The current study aimed at comparing the effect of three placements of the re-exposure episodes on memory retention (interpolated-small, interpolated-medium, postponed), depending on whether retrieval practice or re-reading was used, and on retention interval (one week vs one month).

scholarly journals Efficacy of implementing intermittent STOP THE BLEED® reviews on long term retention of hemorrhage control skills of first year medical students

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Enkhtsogt Sainbayar ◽  
Nathan Holt ◽  
Amber Jacobson ◽  
Shalini Bhatia ◽  
Christina Weaver
Keyword(s):  
Medical Students ◽  
Intervention Group ◽  
Training Session ◽  
Likert Scale ◽  
Control Group ◽  
First Year ◽  
Hemorrhage Control ◽  
Term Retention ◽  
Long Term Retention

Abstract Context Some medical schools integrate STOP THE BLEED® training into their curricula to teach students how to identify and stop life threatening bleeds; these classes that are taught as single day didactic and hands-on training sessions without posttraining reviews. To improve retention and confidence in hemorrhage control, additional review opportunities are necessary. Objectives To investigate whether intermittent STOP THE BLEED® reviews were effective for long term retention of hemorrhage control skills and improving perceived confidence. Methods First year osteopathic medical students were asked to complete an eight item survey (five Likert scale and three quiz format questions) before (pretraining) and after (posttraining) completing a STOP THE BLEED® training session. After the surveys were collected, students were randomly assigned to one of two study groups. Over a 12 week intervention period, each group watched a 4 min STOP THE BLEED® review video (intervention group) or a “distractor” video (control group) at 4 week intervals. After the 12 weeks, the students were asked to complete an 11 item survey. Results Scores on the posttraining survey were higher than the pretraining survey. The median score on the five Likert scale items was 23 points for the posttraining survey and 14 points for the pretraining survey. Two of the three knowledge based quiz format questions significantly improved from pretraining to posttraining (both p<0.001). On the 11 item postintervention survey, both groups performed similarly on the three quiz questions (all p>0.18), but the intervention group had much higher scores on the Likert scale items than the control group regarding their confidence in their ability to identify and control bleeding (intervention group median = 21.4 points vs. control group median = 16.8 points). Conclusions Intermittent review videos for STOP THE BLEED® training improved medical students’ confidence in their hemorrhage control skills, but the videos did not improve their ability to correctly answer quiz-format questions compared with the control group.

Children's long‐term retention is directly constrained by their working memory capacity limitations

2021 ◽  
Author(s):  
Alicia Forsberg ◽  
Dominic Guitard ◽  
Eryn J. Adams ◽  
Duangporn Pattanakul ◽  
Nelson Cowan
Keyword(s):  
Working Memory ◽  
Working Memory Capacity ◽  
Memory Capacity ◽  
Capacity Limitations ◽  
Term Retention ◽  
Long Term Retention
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