scholarly journals Combined Application of Rhizosphere Bacteria with Endophytic Bacteria Suppresses Root Diseases and Increases Productivity of Black Pepper (Piper nigrum L.)

Agriculture ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 15
Author(s):  
Sy Dinh Nguyen ◽  
Thi Huyen Trang Trinh ◽  
Trung Dzung Tran ◽  
Tinh Van Nguyen ◽  
Hoang Van Chuyen ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Black Pepper ◽  
Rhizosphere Bacteria ◽  
Growth And Yield ◽  
Piper Nigrum ◽  
Bacillus Velezensis ◽  
Root Knot Nematode ◽  
Central Highland

Black pepper (Piper nigrum L.) is one of the most important crops and global demand continues to increase, giving it a high export value. However, black pepper cultivation has been seriously affected by a number of pathogenic diseases. Among them, “quick wilt” caused by Phytophthora sp., “slow decline” caused by Fusarium sp., and root-knot nematode Meloidogyne sp. have a serious negative effect on black pepper growth and productivity. There have been different chemical and biological methods applied to control these diseases, but their effectiveness has been limited. The aim of this research was to evaluate different combinations of rhizosphere bacteria and endophytic bacteria isolated from black pepper farms in the Central Highland of Vietnam for their ability to suppress pathogens and promote black pepper growth and yield. Formula 6, containing the strains Bacillus velezensis KN12, Bacillus amyloliquefaciens DL1, Bacillus velezensis DS29, Bacillus subtilis BH15, Bacillus subtilis V1.21 and Bacillus cereus CS30 exhibited the largest effect against Phytophthora and Fusarium in the soil and in the roots of black pepper. These bio-products also increased chlorophyll a and b contents, which led to a 1.5-fold increase of the photosynthetic intensity than the control formula and a 4.5% increase in the peppercorn yield (3.45 vs. 3.30 tons per hectare for the control). Our results suggest that the application of rhizosphere and endophytic bacteria is a promising method for disease control and growth-promotion of black pepper.

scholarly journals POTENSI BAKTERI ENDOFIT MENGINDUKSI KETAHANAN TANAMAN LADA TERHADAP INFEKSI Meloidogyne incognita

2020 ◽  
Vol 17 (3) ◽  
pp. 118 ◽  
Author(s):  
RITA HARNI ◽  
MEYNARTI SARI DEWI IBRAHIM
Keyword(s):  
Root System ◽  
Meloidogyne Incognita ◽  
Endophytic Bacteria ◽  
Black Pepper ◽  
Systemic Resistance ◽  
Piper Nigrum ◽  
Control Methods ◽  
Root Knot Nematode ◽  
Split Root ◽  
Split Root System

<p>ABSTRAK</p><p>Meloidogyne incognita, merupakan salah satu organisme peng-ganggu (OPT) penyebab penyakit kuning pada tanaman lada dan dapatmengakibatkan penurunan hasil sampai 32%. Beberapa teknik untukmengendalikan patogen ini telah dilakukan tetapi belum memberikan hasilyang memuaskan. Pengendalian biologi dengan menggunakan bakteriendofit merupakan salah satu alternatif pengendalian yang cukup men-janjikan untuk dapat mengatasi permasalahan nematoda penyakit tanaman.Penelitian ini telah dilakukan di Laboratorium Bakteriologi danNematologi Departemen Proteksi Tanaman Institut Pertanian Bogor, danRumah Kaca Balai Penelitian Tanaman Rempah dan Aneka TanamanIndustri Pakuwon Sukabumi dari bulan Mei sampai November 2009.Kegiatan yang dilakukan adalah: 1) Seleksi beberapa isolat bakteri endofituntuk mengendalikan nematoda M. incognita pada tanaman lada dan 2)Potensi induced systemic resistance (ISR) dan analisis asam salisilat sertaperoksidase. Isolat bakteri endofit yang digunakan adalah isolat bakteriendofit potensial yang diisolasi dari akar nilam. Akar tanaman ladadirendam dalam suspensi bakteri endofit, selanjutnya diinokulasi dengan500 ekor larva 2 M. incognita. Sebulan setelah inokulasi tanamandibongkar diamati populasi nematoda dan pertumbuhan tanaman. AnalisisISR dilakukan dengan metode split root system dilanjutkan dengananalisis kadar asam salisilat dan peroksidase. Penelitian mengunakanRancangan Acak Lengkap. Hasil penelitian menunjukkan bahwa bakteriendofit dapat menekan jumlah puru dan populasi nematoda di dalam akar.Penekanan tertinggi pada isolat MSK (97,93%) tidak berbeda nyatadengan isolat BAS, TT2, dan NJ46 yaitu 97,35; 95,22; dan 92,14%.Berdasarkan analisis split root system, ke 4 isolat tersebut dapat meng-induksi ketahanan tanaman lada secara sistemik dengan mekanismepeningkatan kandungan asam salisilat dan peroksidase di dalam akar.</p><p>Kata kunci : Bakteri endofit, penyakit kuning, Piper nigrum L.,Meloidogyne incognita, induksi ketahanan</p><p>ABSTRACT</p><p>The use of endophytic bacteria to induce plant resistanceagainst infection of root-knot nematode (Meloidogyneincognita) on black pepper</p><p>Root-knot nematode (Meloidogyne incognita) is one of important patho-gens causing yellow disease on black pepper. As a result of this pathogenattack can lower the results up to 32%. Several control methods have beendone successful to control pathogen. Biological control using endophyticbacteria is one of prospective alternative control methods to overcomenematode problem. The research had been conducted in the Laboratory ofBacteriology and Nematology Department of Plant Protection, BogorAgricultural University (IPB) and in greenhouse of Indonesian Spices andIndustrial Crops Research Institute (ISICRI) Sukabumi. The objectives ofthis study were : 1) Selection of endophytic bacteria to control M.incognita nematodes on black pepper and 2) Potential of induced systemicresistance (ISR) and analysis of salicylic acid and peroxidase. Endophyticbacterial isolates used were endophytic potential bacterial isolates isolatedfrom the roots of patchouli. Pepper plant roots were soaked in anendophytic bacterial suspension, then inoculated with 500 larvae of 2 M.incognita. A month after inoculation, the plants were dismantled andobserved population of nematodes and plant growth. ISR analysis wasperformed by the method of split root system followed by analysis ofsalicylic acid and peroxidase contents. The research was arranged usingCompletely Randomized Design. The results showed that endophyticbacteria were able to suppress the amount of gall and nematode populationin roots. The highest suppression was on MSK isolate (97.93%) which wasnot significantly different from BAS, TT2, and NJ46 isolates, namely97.35, 95.22, and 92.14%, respectively. The analysis of split root systemshowed that the 4 isolates were able to induce systemic resistance of blackpepper with a mechanism of increase in salicylic acid and peroxidasecontents in roots.</p><p>Key words : Endophytic bacteria, yellow disease, Piper nigrum L.,Meloidogyne incognita, induce systemic resistance</p>

Plant growth promotion and fungal antagonism of endophytic bacteria for the sustainable production of black pepper (Piper nigrum L.)

2019 ◽  
Vol 45 (11) ◽  
pp. 5325-5339 ◽  
Author(s):  
Anh Dzung Nguyen ◽  
San-Lang Wang ◽  
Thi Huyen Trang Trinh ◽  
Thi Ngoc Tran ◽  
Van Bon Nguyen ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Black Pepper ◽  
Sustainable Production ◽  
Piper Nigrum ◽  
Fungal Antagonism

A potent antifungal rhizobacteria Bacillus velezensis RB.DS29 isolated from black pepper (Piper nigrum L.)

2019 ◽  
Vol 45 (11) ◽  
pp. 5309-5323 ◽  
Author(s):  
Thi Huyen Trang Trinh ◽  
San-Lang Wang ◽  
Van Bon Nguyen ◽  
Minh Dinh Tran ◽  
Chien Thang Doan ◽  
...  
Keyword(s):  
Black Pepper ◽  
Piper Nigrum ◽  
Bacillus Velezensis

scholarly journals Plant Growth Promotion Rhizobacteria in Onion Production

2014 ◽  
Vol 63 (1) ◽  
pp. 83-88 ◽  
Author(s):  
JOSIP ČOLO ◽  
TIMEA I. HAJNAL-JAFARI ◽  
SIMONIDA ĐURIĆ ◽  
DRAGANA STAMENOV ◽  
SAUD HAMIDOVIĆ
Keyword(s):  
Acetic Acid ◽  
Bacillus Subtilis ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Indole Acetic Acid ◽  
Growth Promotion ◽  
Azotobacter Chroococcum ◽  
Growth And Yield ◽  
Rhizospheric Bacteria ◽  
Number Of Bacteria

The aim of the research was to examine the effect of rhizospheric bacteria Azotobacter chroococcum, Pseudomonas fluorescens (strains 1 and 2) and Bacillus subtilis on the growth and yield of onion and on the microorganisms in the rhizosphere of onion. The ability of microorganisms to produce indole-acetic acid (IAA), siderophores and to solubilize tricalcium phosphate (TCP) was also assessed. The experiment was conducted in field conditions, in chernozem type of soil. Bacillus subtilis was the best producer of IAA, whereas Pseudomonas fluorescens strains were better at producing siderophores and solubilizing phosphates. The longest seedling was observed with the application of Azotobacter chroococcum. The height of the plants sixty days after sowing was greater in all the inoculated variants than in the control. The highest onion yield was observed in Bacillus subtilis and Azotobacter chroococcum variants. The total number of bacteria and the number of Azotobacter chroococcum were larger in all the inoculated variants then in the control. The number of fungi decreased in most of the inoculated variants, whereas the number of actinomycetes decreased or remained the same.

scholarly journals Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 286 ◽  
Author(s):  
Van Anh Ngo ◽  
San-Lang Wang ◽  
Van Bon Nguyen ◽  
Chien Thang Doan ◽  
Thi Ngoc Tran ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Endophytic Bacteria ◽  
Black Pepper ◽  
Antagonistic Activity ◽  
Piper Nigrum ◽  
Control Group ◽  
Vitro Assay ◽  
16S Rna

In this study, 90 root samples were collected from 30 black pepper farms in three provinces in the Central Highlands of Vietnam. A total of 352 endophytic bacteria were isolated and their morphology described. An in vitro assay on the antifungal activity of these isolates was then conducted and 47 isolates were found to have antagonistic activity on Phytophthora fungi. The antifungal activity of the 47 isolates was evaluated in vivo by shoot assay. Among these 47 isolates, 6 were selected for further investigation. The six isolates were classified and identified by sequencing the 16S RNA gene and phylogeny. The results showed that all six endophytic bacteria belong to the following species of Bacillus genus: B. siamensis, B. amyloliquefaciens, B. velezenis, and B. methylotrophiycus. Enzymatic activity related to the antifungal activity of the six potent isolates was determined; it showed that they possessed high chitinase and protease activities. These isolates were applied for black pepper seedlings in greenhouse. The results showed three promising isolates: B. siamensis EB.CP6, B. velezensis EB.KN12, and B. methylotrophycus EB.KN13. Black pepper seedlings treated with the promising bacteria had the lowest rate of root disease (8.45–11.21%) and lower fatal rate (11.11–15.55%) compared to the control group (24.81% and 24.44%). In addition, the three promising isolates strongly affected the growth of the black pepper seedlings in greenhouse. The plant height, length of roots, and fresh biomass of the seedlings in the treated plots were higher than those in the control plots. Thus, the endophytic bacterial isolates have the potential to act as biocontrol agent for the sustainable production of black pepper.

scholarly journals Current status of plant parasitic nematodes and their management of major spice crops

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Rashid Pervez
Keyword(s):  
Curcuma Longa ◽  
Phytophthora Capsici ◽  
Brown Rot ◽  
Black Pepper ◽  
Current Status ◽  
Piper Nigrum ◽  
Parasitic Nematodes ◽  
Root Knot Nematode ◽  
Meloidogyne Spp ◽  
Lesion Nematode

Major spices crops such as black pepper (Piper nigrum L.), cardamom (Elettaria cardamomum Maton.) and turmeric (Curcuma longa L.) production in India is sustained losses due to several reasons. Among them, one of the major constraints are nematode infesting diseases, which causes significant yield losses and affecting their productivity. The major nematode pests infesting these crops include burrowing nematode Radopholus similis; root knot nematode, Meloidogyne incognita and M. javanica on black pepper. Whereas, lesion nematode, Pratylenchus sp., M. incognita and R. similis infesting cardamom and turmeric crops. Black pepper is susceptible to a number of diseases of which slow decline caused by R. similis and M. incognita or Phytophthora capsici  either alone and in combination and root knot disease caused by Meloidogyne spp. are the major ones.  Root knot disease caused by Meloidogyne spp. is major constraints in the successful cultivation and production in cardamom. Turmeric is susceptible to a number of diseases such as brown rot disease is caused by Fusarium sp. and lesion nematode, Pratylenchus sp. and root knot disease caused by M. incognita. Adoption of integrated pest management schedules is important in these crops since excessive use of pesticides could lead to pesticide residues in the produce affecting human health and also causing other ecological hazards. 

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