scholarly journals Distribution of Pigeon Pea Cyst Nematode and Root-Knot Nematodes in Major Sesame Growing Areas in Myanmar

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1457
Author(s):  
Yu Yu Min ◽  
Thu Htet Naing ◽  
Nwe Nwe Htun ◽  
Aung Kyaw Myint ◽  
Yuri Ichinose ◽  
...  
Keyword(s):  
Parasitic Nematode ◽  
Pigeon Pea ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Nematode Density ◽  
Root Knot Nematodes ◽  
Cropping Patterns ◽  
Meloidogyne Spp ◽  
The Mean ◽  
Heterodera Cajani

The pigeon pea cyst nematode, Heterodera cajani, is an economically important plant-parasitic nematode in sesame production. The objective of the study was to investigate distribution of H. cajani in the major sesame producing areas in Myanmar. Before cultivation, soil samples were collected in 96 local farmer’s fields: (19 fields in Magway, 20 fields in Mandalay, 14 fields in Sagaing and 43 in Nay Pyi Taw), and nematode density was quantified by real-time PCR. The cyst nematode was detected in 63.2% of the fields in Magway and the mean density was 22.6 eggs equivalent (20 g soil)−1. In Mandalay and Nay Pyi Taw, the cyst nematode was detected in 40.0% and 18.6% of the fields with the mean densities of 9.9 and 21.0 eggs equivalent (20 g soil)−1, respectively. By contrast, the cyst nematode was not detected in Sagaing fields, in which sesame is rotated with paddy rice. Infestation of root-knot nematodes (RKN, Meloidogyne spp.) was also evaluated, because sesame is generally rotated with rice and pulses, which are attacked by RKN. The results showed 25.6% infestation of RKN with a mean density of 34.3 eggs equivalent (20 g soil)−1 only in Nay Pyi Taw, while there was no infestation in Magway, Mandalay, and Sagaing. The relationships between the nematode infestation level and the cropping patterns in each sesame growing area in Myanmar were discussed.

scholarly journals Survey of Wilt (Fusarium udum) and the Cyst Nematode (Heterodera cajani) Incidence on Pigeonpea of Prayagraj District

2020 ◽  
pp. 23-28
Author(s):  
Amit Kumar Maurya ◽  
Sobita Simon ◽  
Vinny John ◽  
Abhilasha A. Lal
Keyword(s):  
Disease Incidence ◽  
Mesh Size ◽  
Pigeon Pea ◽  
Cyst Nematode ◽  
Wilt Disease ◽  
Soil Samples ◽  
Fusarium Udum ◽  
Pump House ◽  
Very High ◽  
Heterodera Cajani

A survey was conducted in selected villages of Prayagraj district to evaluate the disease incidence of Fusarium (Fusarium udum) wilt and cyst nematode (Heterodera cajani) of pigeon pea (Cajanus cajan). Soil and roots were collected from the rhizosphere of infested pigeonpea plants. The maximum wilt incidence was recorded (57.6%) in Dari village followed by Pump House (56%), Pahlu ka Purwa (51.2%) and the lowest incidence of wilt was recorded in Chaka (12.8%), Naini (11.2%) and Jhusi (9.6%) in the year 2016. For cyst nematode, soil samples was collected from selected fields, the soil was mixed thoroughly and 500 g of the soil was processed following Cobb’s sieving and decanting method where cyst and J2’s were separated in separate mesh size sieves. It was observed that infestation of Heterodera cajani was very high in Jari (51%) followed by Naini (41%). The minimum infestation of Heterodera cajani was found in Meza village which was responsible for wilt disease in pigeonpea concluding that Fusarium wilt of pigeonpea is a major problem in Prayagraj district of U.P. India.

Response of soybean cyst nematode (Heterodera glycines) and root-knot nematodes (Meloidogyne spp.) to gradients of pH and inorganic salts

2020 ◽  
Vol 455 (1-2) ◽  
pp. 305-318
Author(s):  
Cui Hua ◽  
Chunjie Li ◽  
Ye Jiang ◽  
Minghui Huang ◽  
Valerie M. Williamson ◽  
...  
Keyword(s):  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Inorganic Salts ◽  
Root Knot Nematodes ◽  
Meloidogyne Spp

Further Notes On the Pigeon-Pea Cyst Nematode, Heterodera Cajani

Nematologica ◽  
1970 ◽  
Vol 16 (4) ◽  
pp. 477-482 ◽  
Author(s):  
C.L. Sethi ◽  
P.K. Koshy ◽  
G. Swarup
Keyword(s):  
Pigeon Pea ◽  
Cyst Nematode ◽  
Heterodera Cajani

Plant parasitic nematodes in white clover and soil from white clover pastures in Australia

1997 ◽  
Vol 37 (1) ◽  
pp. 75 ◽  
Author(s):  
L. J. McLeish ◽  
G. N. Berg ◽  
J. M. Hinch ◽  
L. V. Nambiar ◽  
M. R. Norton
Keyword(s):  
White Clover ◽  
Parasitic Nematode ◽  
Cyst Nematode ◽  
Parasitic Nematodes ◽  
Root Lesion Nematode ◽  
Plant Parasitic Nematodes ◽  
Meloidogyne Spp ◽  
Trifolium Repens L ◽  
Lesion Nematode ◽  
Plant Parasitic

Summary. Seventeen sites, including locations in all the major white clover growing regions of Australia, were surveyed for the presence of plant parasitic nematodes in autumn and spring 1993. Trifolium repens L. cvv. Haifa and Irrigation, plus 1 other cultivar, were sampled at each site and nematodes extracted from roots, stems and soil. Thirteen genera of plant parasitic nematodes were detected. The clover cyst nematode, Heterodera trifolii, and root knot nematodes, Meloidogyne spp., were each recorded at over 75% of the sites. The most common genera of plant parasitic nematodes detected were Tylenchus, which was present at all sites, and Pratylenchus (root lesion nematode), which was present at all but 1 site. Other plant parasitic nematode genera found included Ditylenchus, Helicotylenchus and Paratylenchus. The widespread presence of nematodes in white clover pastures, and the high populations at some sites, suggest that they may be economically important to the Australian dairy industry.

Molecular diagnostics of the pigeon pea cyst nematode, Heterodera cajani Koshy, 1967, using real-time PCR

Nematology ◽  
2021 ◽  
pp. 1-6
Author(s):  
Tatiana V. Roubtsova ◽  
Sergei A. Subbotin
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pigeon Pea ◽  
Cyst Nematode ◽  
Taqman Probe ◽  
28S Rrna ◽  
Specific Primer ◽  
Pcr Assay ◽  
Species Specific ◽  
Heterodera Cajani

Summary The pigeon pea cyst nematode, Heterodera cajani, is an important nematode pest of pigeon pea that is present in all major growing regions of this crop in India and reported from Pakistan, Egypt and Myanmar. In this study, a new real-time PCR assay for detection of H. cajani using a species-specific primer and a TaqMan probe was developed. The primers and a probe were designed to amplify the COI gene fragment. The specificity of the primer-probe set was tested in singleplex or multiplex reactions against target and non-target nematodes. In multiplex real-time PCR experiments with the specific and universal primer-probe sets, the signals were simultaneously observed for COI and D3 of 28S rRNA target genes. The results showed that the real-time PCR assay with species-specific primer and probe was sensitive enough to detect H. cajani DNA extracted from 0.003 egg or second-stage juvenile.

scholarly journals Potato Cyst Nematode in East Java: Newly Infected Areas and Identification

2018 ◽  
Vol 21 (2) ◽  
pp. 87 ◽  
Author(s):  
Happy Cahya Nugrahana ◽  
Siwi Indarti ◽  
Edhi Martono
Keyword(s):  
Sea Level ◽  
Parasitic Nematode ◽  
Nematode Species ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Molecular Approaches ◽  
Plant Parasitic

Potato Cyst Nematodes (PCN), Globodera rostochiensis has noted to be a devastated pest on potato in Indonesia. It is listed as the A2 pest by Plant Quarantine of Republik Indonesia, and it was also being a highly concerned plant parasitic nematode species worlwide. Therefore, both intensive and extensive surveys should be done to monitor the spread of PCN, especially in East Java as one of the centre of potato plantations in Indonesia. The aim of this study was to study the distribution of PCN in four potato plantations in East Java, i.e. Batu, Magetan, Probolinggo, and Pasuruan which were located between 1,205 to 2,063 m above the sea level. Extraction and isolation of cysts from soil samples was done using Baunacke method, and it was followed by identification of the nematodes using morphological and molecular approaches according to Baldwin and Mundo-Ocampo. The results showed that PCN was found on all sampling sites, i.e. Batu (Sumber Brantas, Jurang Kuali, Tunggangan, Junggo, Brakseng); Magetan (Dadi, Sarangan, Singolangu); Probolinggo (Tukul, Pandansari, Ledokombo, Sumberanom, Wonokerto, Ngadas), Pasuruan (Wonokerto, Tosari, Ledoksari, Ngadiwono). Magetan and Pasuruan were noted as new infested areas in East Java. Both morphological and molecular methods showed that the species found on all sites was Globodera rostochiensis. IntisariNematoda Sista Kentang (NSK), Globodera rostochiensis telah tercatat sebagai hama yang menghancurkan tanaman kentang di Indonesia. NSK terdaftar sebagai Organisme Pengganggu Tumbuhan Karantina golongan A2 oleh Badan Karantina Pertanian Republik Indonesia, dan juga merupakan spesies nematoda parasit tanaman yang sangat merugikan di seluruh dunia. Oleh karena itu, baik survei intensif maupun ekstensif harus dilakukan untuk memantau penyebaran NSK, terutama di Jawa Timur sebagai salah satu sentra tanaman kentang di Indonesia. Tujuan dari penelitian ini adalah untuk mempelajari distribusi NSK pada empat daerah sentra penanaman kentang di Jawa Timur, yaitu Batu, Magetan, Probolinggo, dan Pasuruan yang terletak antara 1.205 sampai 2.063 m di atas permukaan laut. Ekstraksi dan isolasi sista NSK dari sampel tanah dilakukan dengan metode Baunacke, dan dilanjutkan dengan identifikasi secara morfologi dan molekuler menurut Baldwin dan Mundo-Ocampo. Hasil penelitian menunjukkan bahwa NSK ditemukan di semua lokasi pengambilan sampel, yaitu Batu (Sumber Brantas, Jurang Kuali, Tunggangan, Junggo, Brakseng); Magetan (Dadi, Sarangan, Singolangu); Probolinggo (Tukul, Pandansari, Ledokombo, Sumberanom, Wonokerto, Ngadas), Pasuruan (Wonokerto, Tosari, Ledoksari, Ngadiwono). Magetan dan Pasuruan tercatat sebagai daerah sebaran baru di Jawa Timur. Hasil identifikasi secara morfologi dan molekuler menunjukkan bahwa spesies yang ditemukan di semua lokasi adalah Globodera rostochiensis.

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