scholarly journals Breeding for Enhancing Legumovirus Resistance in Mungbean: Current Understanding and Future Directions

Agronomy ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 622 ◽  
Author(s):  
Chandra Mohan Singh ◽  
Poornima Singh ◽  
Aditya Pratap ◽  
Rakesh Pandey ◽  
Shalini Purwar ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Seed Quality ◽  
Soil Health ◽  
Mosaic Disease ◽  
Bipartite Begomoviruses ◽  
Yellow Mosaic Virus ◽  
Symbiotic Association ◽  
Detection Techniques ◽  
Vigna Species ◽  
Mungbean Yellow Mosaic Virus

Yellow mosaic disease (YMD) affects several types of leguminous crops, including the Vigna species, which comprises a number of commercially important pulse crops. YMD is characterized by the formation of a bright yellow mosaic pattern on the leaves; in severe forms, this pattern can also be seen on stems and pods. This disease leads to tremendous yield losses, even up to 100%, in addition to deterioration in seed quality. Symptoms of this disease are similar among affected plants; YMD is not limited to mungbean (Vigna radiata L. Wilczek) and also affects other collateral and alternate hosts. In the last decade, rapid advancements in molecular detection techniques have been made, leading to an improved understanding of YMD-causing viruses. Three distinct bipartite begomoviruses, namely, Mungbean Yellow Mosaic India Virus (MYMIV), Mungbean Yellow Mosaic Virus (MYMV), and Horsegram Yellow Mosaic Virus (HgYMV), are known to cause YMD in Vigna spp. Vigna crops serve as an excellent protein source for vegetarians worldwide; moreover, they aid in improving soil health by fixing atmospheric nitrogen through a symbiotic association with Rhizobium bacteria. The loss in the yield of these short-duration crops due to YMD, thus, needs to be checked. This review highlights the discoveries that have been made regarding various aspects of YMD affecting mungbean, including the determination of YMD-causing viruses and strategies used to develop high-yielding YMD-resistant mungbean varieties that harness the potential of related Vigna species through the use of different omics approaches.

​RCA-based Detection of Begomoviruses in Weed Ggenera Associated with Legumes in Southern Karnataka

2021 ◽  
Author(s):  
Sudeep Pandey ◽  
T.R. Girish ◽  
S. Basavaraj ◽  
A.S. Padmaja ◽  
N. Nagaraju
Keyword(s):  
Mosaic Virus ◽  
Rolling Circle Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Insect Vector ◽  
Weed Species ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Mungbean Yellow Mosaic Virus ◽  
Legume Crops

Background: Yellow mosaic disease (YMD) caused by begomoviruses transmitted through the insect vector Bemisia tabaci poses a serious threat to the production of legume crops. Methods: Season-long surveys were carried out for YMD occurrence in six different legume crops and associated natural weeds both symptomatic and asymptomatic across the districts of southern Karnataka, India. The samples were analyzed through RCA PCR using specific primer pairs. Result: Up to 94.1 per cent YMD incidence was recorded and nine weed species were commonly found associated with legume crops. The weeds viz., Ageratum conyzoides, Alternanthera sessilis, Commelina benghalensis and Euphorbia geniculata were abundantly found in the surveyed regions. The weeds were both symptomatic and asymptomatic. Rolling circle amplification coupled polymerase chain reaction method was employed to detect yellow mosaic virus in asymptomatic weeds. Phylogenetic analysis based on the sequences of PCR amplified products of weeds and symptomatic legumes revealed a close clustering of the weed samples with horsegram yellow mosaic virus, legume yellow mosaic virus and mungbean yellow mosaic virus. Overall, our data suggests the role of weed species associated with legume crops as alternative/collateral hosts of begomoviruses and their role in the epidemiology of yellow mosaic disease.

scholarly journals Management of Yellow Mosaic Disease of Mungbean Against Mungbean Yellow Mosaic Virus (MYMV) in Bangladesh

2018 ◽  
Vol 1 (2) ◽  
pp. p100
Author(s):  
Md. S. Islam ◽  
Md. B. Hossain ◽  
Saleh A. Shahriar ◽  
Fatema Begum ◽  
Md. N. H. Sani
Keyword(s):  
Mosaic Virus ◽  
Disease Severity ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Economic Damage ◽  
Yellow Mosaic Disease ◽  
Yield Attributes ◽  
Mungbean Yellow Mosaic Virus

The prime aim of the study was to manage of Yellow mosaic disease of mungbean against Mungbean yellow mosaic virus (MYMV) by using one newly release botanical nutrient and through three selected insecticides. BARI (Bangladesh Agricultural Research Institute) released variety BARI mung-5, three insecticides (Imidacloprid, Acmix and Sobicron) and one botanical nutrient PPN (Peak performance nutrients) were used in the experiment. The plants were grown for pulse production and natural inoculums were relied upon for the infection of MYMV. Growth parameters, yield attributes and physiological features were significantly influenced by the application of selected insecticides and PPN combinations. Disease incidence and disease severity of MYMV were significantly varied among the treatments. Application of Imidacloprid with PPN combination gave the lowest disease incidence (3.13, 5.24 and 6.24% per plot and 14.33, 15.49 and 21.87% per plant) at 30, 40 and 50 DAS, respectively while the highest disease incidence (7.77, 13.70 and 19.24% per plot and 39.33, 48.20 and 56.63% per plant) were found in control at 30, 40 and 50 DAS, respectively. Application of Imidacloprid with PPN also gave the lowest disease severity (5.00, 6.00 and 13.33% at 30, 40 and 50 DAS, respectively while the highest disease severity (27.33, 35.00 and 45.00%) at 30, 40 and 50 DAS, respectively were measured in control treatment when no insecticides and PPN was used. If the disease is established once in the field then it is difficult to manage. As the disease is transmitted by vector (whitefly), the growers are suggested to control the vector populations before reaching economic damage and severe disease infection.

scholarly journals Seed Borne Nature of Mungbean yellow mosaic virus (MYMV) in Blackgram in Tamil Nadu

2021 ◽  
pp. 58-67
Author(s):  
K. Kamesh Krishnamoorthy ◽  
V. G. Malathi ◽  
P. Renukadevi ◽  
S. Mohan Kumar ◽  
M. Raveendran ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Tamil Nadu ◽  
Infected Plant ◽  
Susceptible Variety ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Resistant Varieties ◽  
Mungbean Yellow Mosaic Virus ◽  
Cropping Seasons ◽  
Agroclimatic Zones

The yellow mosaic disease of blackgram caused by Mungbean yellow mosaic virus has emerged as a serious threat to pulses production especially in the South Eastern Asia.  Seed borne nature of MYMV in blackgram seeds was determined using the seeds harvested from a MYMV resistant (either VBN-6 or VBN-8) and susceptible blackgram (CO-5) varieties grown in three different agroclimatic zones of Tamil Nadu in India for three consecutive cropping seasons namely, Rabi 2018 (October- December), Summer 2019 (March-May) and Kharif 2019 (June- August) at three different time intervals viz., 20, 40 and 60 days after sowing (DAS). Seed borne nature of MYMV was observed only in the susceptible variety CO-5 and was absent in the   resistant varieties. Transmission of MYMV from infected plant to seeds was observed in all the three parts of the seeds viz., seed coat, cotyledon and embryo. Seeds from infected plants also showed abnormalities like shrinking, discolouration, ill filling inside pods and misshapen appearance.

Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek

2018 ◽  
Author(s):  
Prince Lekhi ◽  
R. K. Gill ◽  
Satinder Kaur ◽  
T. S. Bains
Keyword(s):  
Microsatellite Markers ◽  
Mosaic Virus ◽  
Vigna Radiata ◽  
Female Parent ◽  
Germination Percentage ◽  
Yellow Mosaic Virus ◽  
F1 Hybrids ◽  
Polymorphic Markers ◽  
Vigna Species ◽  
Mungbean Yellow Mosaic Virus

Vigna radiata genotypes viz., SML 668 and SML 832 and V. mungo genotypes viz., Mash 114 and Mash 218 were crossed in all possible combinations during summer 2015 to generate F1 hybrids. Interspecific hybridization was attempted by using V. radiata genotypes as female parent. Pod set percentage varied from 5.5 percent (SML 832 x Mash 218) to 24.1 percent (SML 832 x Mash 114). The germination percentage ranged from 14.29 to 30.56. Maximum pollen fertility was observed in cross SML 668 x Mash 114 (28.36 percent) followed by SML 668 x Mash 218 (27.03 percent), SML 832 x Mash 218 (24.32 percent) and minimum in SML 832 x Mash 114 (22.59 percent).The purity of hybrids were tested through microsatellite markers. For parental polymorphism, microsatellite markers were selected from related Vigna species such as Vigna unguiculata, Vigna radiata and Vigna mungo. Out of 84 markers used, 46 were polymorphic i.e 54.76 per cent polymorphism between parents. These polymorphic markers were used for confirmation of hybrids produced from different crosses. All the F1 plants gave resistant reaction to Mungbean yellow mosaic virus (MYMV) indicating the introgression of resistance gene(s) from V. mungo to V. radiata.

Identification of stable sources of resistance to mungbean yellow mosaic virus (MYMV) disease in mungbean [Vigna radiata (L.) Wilczek]

2019 ◽  
Vol 17 (04) ◽  
pp. 362-370
Author(s):  
Nagaraj ◽  
S Basavaraj ◽  
A.S. Padmaja ◽  
N Nagaraju ◽  
S Ramesh
Keyword(s):  
Mosaic Virus ◽  
Natural Infection ◽  
Rolling Circle Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Sources Of Resistance ◽  
Resistance Response ◽  
Rolling Circle ◽  
Mungbean Yellow Mosaic Virus ◽  
Cent Disease

AbstractYellow mosaic disease (YMD) caused by mungbean yellow mosaic virus (MYMV) is one of the most destructive biotic production constraints in mungbean. Development and introduction of resistant cultivars are considered as the most economical and eco-friendly option to manage YMD, for which availability of stable sources of resistance is a pre-requisite. A set of 14 mungbean genotypes including a susceptible check were evaluated for responses to YMD under natural infection across three seasons and under challenged inoculation in glasshouse for one season. None of the genotypes were immune to YMD and produced different degrees of response to MYMV in terms of yellow mosaic symptoms (YMS). Based on the delayed appearance of initial YMS, and lower estimates of per cent disease index and area under disease progressive curve (AUDPC) in response to natural infection and challenged inoculation, five genotypes namely AVMU 1698, AVMU 1699, AVMU 16100, AVMU 16101 and KPS 2 were identified as resistant to YMD. Failure of detection of MYMV through polymerase chain reaction (PCR) using MYMV coat protein gene-specific primer and successful detection of the same through rolling circle amplification-PCR suggested latent infection of MYMV in resistant genotypes. The resistance response of the five genotypes could be attributed to enhanced activities of enzymes such as peroxidase, polyphenol oxidase and phenylalanine ammonia lyase and increased concentration of total phenols. These results are discussed in relation to strategies to breed mungbean for resistance to YMD.

Search for Vigna species conferring resistance to Mungbean yellow mosaic virus in mungbean

2014 ◽  
Vol 13 (2) ◽  
pp. 162-167 ◽  
Author(s):  
M. Sudha ◽  
A. Karthikeyan ◽  
V. G. Shobhana ◽  
P. Nagarajan ◽  
M. Raveendran ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Pcr Analysis ◽  
Quantitative Detection ◽  
Interspecific Crosses ◽  
Rt Pcr ◽  
Vigna Species ◽  
Resistant Varieties ◽  
Mungbean Yellow Mosaic Virus ◽  
Polymerase Chain

Mungbean yellow mosaic virus (MYMV) is a disastrous pathogen of mungbean. It is widespread in most of southern India and no complete resistance has been identified among its commercial cultivars. Two isolates of MYMV, representing its diversity, were used to assess and characterize the susceptibility reaction of all the three species of Vigna. The seeds were agroinoculated with the virus and the presence of the viral DNA was confirmed after 12 d by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis after which the plants were monitored for the expression of symptoms. All of the 20 accessions of Vigna radiata and ten accessions of Vigna mungo were systemically infected with MYMV, and they all produced typical symptoms. On the other hand, the 24 accessions of Vigna umbellata were found to be resistant to both the isolates. For additional affirmation, three representative accessions of V. radiata and V. mungo and all the accessions of V. umbellata were agroinoculated, and quantitative RT-PCR was performed for the quantitative detection of the MYMV. The mRNA transcripts of MYMV were detected in V. radiata and V. mungo plants but not in the V. umbellata plants. Researching the molecular basis of the resistance in V. umbellate against MYMV might definitely be very constructive for developing resistant varieties of mungbean on a commercial scale. This genetic quality offering resistance to MYMV could also be incorporated into V. radiata/V. mungo by means of interspecific crosses.

scholarly journals Screening of Urdbean Germplasm for High Seed Yield Coupled with Resistance to Mungbean yellow mosaic virus (MYMV)

2020 ◽  
pp. 100-106 ◽  
Author(s):  
Sunita Kumari ◽  
Sangita Sahni ◽  
Bishun Deo Prasad
Keyword(s):  
Mosaic Virus ◽  
Seed Yield ◽  
Disease Incidence ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Sources Of Resistance ◽  
Production Constraints ◽  
Mungbean Yellow Mosaic Virus ◽  
Disease Free ◽  
High Seed Yield

Yellow mosaic disease (YMD) caused by Mungbean yellow mosaic virus (MYMV) is one of the most destructive biotic production constraints in urdbean. Development and introduction of resistant cultivars with high seed yield are considered as the most economical and eco-friendly option to manage YMD, for which availability of stable sources of resistance with high seed yield is a pre-requisite. A set of one hundred twenty eight genotypes of urdbean including a susceptible check were evaluated against MYMV in the field for two consecutive years during summer and kharif 2015-2016 under natural condition of disease incidence. There was considerable variation among the genotypes with respect to disease reaction. Out of 128 genotypes tested, only five genotype namely KU 96-3, NDU 12-1,  NIRB 002,  NIRB 003 & NIRB 004 were found to be disease free, nineteen genotypes (IPU 10-23,  IPU 11-01, KPU 34, KUG 540,  KUG 586, Mash-338, NDU 12-2, NDU 12-300, NDUZ 14-21,OBG 35,  PU 09-35, Shekhar 3,  UH 07-06, Uttara, VBG 10-008, VBG 11-053,VBN (BG) 3, VBN 6 & Vijay) found to be highly resistant and twenty two genotypes (IGKU 02-1, Kopergaon,  KPU 12, KPU 13, KPU 14, KPU 16, KPU 33, KPU 7, KPU 8, KU 363,  NDU 11-01,Palampur 93, Pant U 19,  PU 08-05, PU 20, PU 22, RUG-44, Sekhar 2 , TU 67, UG 218, VBG 09-005 & WBU 108) showed highly resistance or resistance consistently in both the seasons. However, eight genotypes i.e. Uttara, PU-31, KU-363, KUG 540, UH 07-06, KUG 503, WBU 108 & Shekhar 33 were found to be superior for seed yield as well as resistant to MYMV. Among these lines, UH 07-06 and KUG 503 give highest seed yield in Kharif and summer.

scholarly journals Screening of Mungbean (Vignaradiata L. Wilczek) Genotypes for Resistance to Mungbean Yellow Mosaic Virus under Field Condition

2021 ◽  
pp. 39-45
Author(s):  
Yugandhar Gokidi ◽  
M. N. Singh ◽  
Ashok Singamsetti ◽  
Swathi Lekkala
Keyword(s):  
Mosaic Virus ◽  
Field Condition ◽  
Seed Quality ◽  
Disease Incidence ◽  
Pigeon Pea ◽  
Yellow Mosaic Virus ◽  
Grain Legume ◽  
Legume Crop ◽  
Mungbean Yellow Mosaic Virus ◽  
The Difference

Greengram [Vignaradiata(L.) Wilczek] is an economically important grain legume crop next to chickpea, pigeon pea and urad gram. Among the biotic factors, Mungbean Yellow Mosaic Virus (MYMV) is reported to be the most destructive viral diseases, which may reduce the seed quality as well as the yield losses up to 100 per cent. It is transmitted through whitefly (Bemisiatabaci) in a persistent manner throughout Asia, including India. A set of forty-two diverse mungbean genotypes were sown in two replications using a Randomized Block Desigh (RBD) during the summer season of 2015. The infector row method was adopted to evaluate a set of mungbean genotypes to know the difference in the level of resistance against MYMV infection under field condition during summer, 2015. Percent Disease Incidence (PDI) was calculated at 30 DAS and 50 DAS respectively. It varied from 2.18 to 64.77% and 5.38 to 76.87% at 30 DAS and 50 DAS respectively in summer, 2015. On the basis of disease severity recorded, the mungbean genotypes were classified in to five disease infection categories. Out of the forty-two mungbean genotypes, thirteen genotypes viz., Pusa 0672, IPM 205-7, HUM 8, KM 2245, IPM-2-03, ML 1464, KM 2241, PDM-139, TARM-1, HUM 26, Meha, HUM 16 and IPM 409-4 were found to be resistant and may provide the source of resistance against MYMV to develop mapping population for molecular breeding, development of molecular markers, QTL identification for MYMV resistance, as well as development of MYMV resistant varieties.

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