scholarly journals Nasal Administration of Lipopolysaccharide Exacerbates Allergic Rhinitis through Th2 Cytokine Production from Mast Cells

Allergies ◽  
2021 ◽  
Vol 1 (4) ◽  
pp. 216-224
Author(s):  
Noriaki Aoi ◽  
Takafumi Fuchiwaki ◽  
Ichiro Morikura ◽  
Hideyuki Kawauchi ◽  
Tatsunori Sakamoto
Keyword(s):  
Allergic Rhinitis ◽  
Clinical Practice ◽  
Mast Cells ◽  
Nasal Mucosa ◽  
Cytokine Production ◽  
Nasal Administration ◽  
Eosinophil Infiltration ◽  
Dependent Manner ◽  
Th2 Cytokine ◽  
Nasal Symptoms

Background: Microbial infection or exposure to endotoxin later in life exacerbates established asthma. Mast cells are involved in the exacerbation of asthma. This exacerbation involves a toll-like receptor (TLR)–mediated response of mast cells. In the clinical practice of otolaryngology, otolaryngologists experience an exacerbation of nasal congestion when infectious rhinitis develops in patients with allergic rhinitis, but the mechanisms are unknown. Therefore, this study investigated the effect of lipopolysaccharide (LPS) on allergic rhinitis using a mouse allergic rhinitis model. Methods: Female BALB/c mice, TLR4 gene mutant C3H/HeJ mice or mast cell–deficient WBB6F1-W/Wv mice were sensitized intraperitoneally with ovalbumin (OVA)/alum, and were intranasal challenged with OVA and/or LPS. Nasal symptoms and histologic changes were examined. Cytokines in nasal tissue were examined by Western blot. The effects of LPS on degranulation and cytokine production of bone marrow–derived mast cells (BMMCs) were investigated. Results: Nasal administration of LPS together with the antigen exacerbated nasal symptoms, eosinophil infiltration of the nasal mucosa, and increased IL-5 production in the nasal mucosa. It was not observed in C3H/HeJ mice and WBB6F1-W/Wv mice. The addition of LPS increased the production of IL-5 from BMMCs in a dose-dependent manner, but no effect on degranulation was observed. Conclusions: Intranasal administration of LPS exacerbates allergic rhinitis through Th2 cytokine production from mast cells. This observation provides clues to the mechanism of exacerbation of allergic rhinitis caused by an infection in daily clinical practice.

Effects of TAM (Taraxacum mongolicum) on Th2 Cytokine Production in MC/9 Mast Cells

2012 ◽  
Vol 24 (1) ◽  
pp. 54-65 ◽  
Author(s):  
Moon-Hee Jang ◽  
Jae-Song Choi ◽  
Na-Young Bae ◽  
Teak-Won Ahn
Keyword(s):  
Mast Cells ◽  
Cytokine Production ◽  
Th2 Cytokine

scholarly journals Interleukin 10 inhibits lipopolysaccharide-induced survival and cytokine production by human peripheral blood eosinophils.

1994 ◽  
Vol 180 (2) ◽  
pp. 711-715 ◽  
Author(s):  
S Takanaski ◽  
R Nonaka ◽  
Z Xing ◽  
P O'Byrne ◽  
J Dolovich ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Cytokine Production ◽  
Human Peripheral Blood ◽  
Interleukin 10 ◽  
Activation Mechanism ◽  
Eosinophil Infiltration ◽  
Dependent Manner ◽  
Gm Csf ◽  
Eosinophil Survival ◽  
Blood Eosinophils

In this study we have investigated the effects of interleukin 10 (IL-10) on human peripheral blood eosinophils stimulated with granulocyte/macrophage colony stimulating factor (GM-CSF) and lipopolysaccharide (LPS). We show that LPS was able to enhance eosinophil survival in a dose-dependent manner, as well as release of the cytokines GM-CSF, tumor necrosis factor alpha, and IL-8. LPS-induced eosinophil survival was largely inhibited by an anti-GM-CSF neutralizing antibody and completely blocked by polymyxin B, suggesting GM-CSF involvement in the survival enhancing mechanism and LPS specificity, respectively. IL-10 significantly inhibited survival of, and cytokine production from, eosinophils induced by LPS, but did not inhibit the survival induced by GM-CSF. These observations suggest a novel activation mechanism of eosinophils and, also, that IL-10 may participate in the regulation of diseases characterized by eosinophil infiltration.

Preventive Effect of Bupleurum chinense on Nasal Inflammation via Suppressing T Helper Type 2, Eosinophil and Mast Cell Activation

2019 ◽  
Vol 47 (02) ◽  
pp. 405-421 ◽  
Author(s):  
Thi Tho Bui ◽  
Chun Hua Piao ◽  
Eunjin Hyeon ◽  
Yanjing Fan ◽  
Dae Woon Choi ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cells ◽  
Nasal Mucosa ◽  
Cell Activation ◽  
Therapeutic Potential ◽  
Specific Effect ◽  
Lavage Fluid ◽  
Mast Cell Activation ◽  
Nasal Allergy ◽  
Independent Manner

Bupleurum chinense is distributed in East Asia and has been used as a traditional herbal medicine for more than a thousand years. Though B. chinense has been reported to have immunomodulatory, anti-inflammatory, anti-oxidant, hepato-protective, antipyretic, analgesic and antifibrotic effects, its specific effect on allergic rhinitis disease has not been clarified. In this study, we investigated the anti-allergic and anti-inflammation effects of B. chinense extract (BCE) in an ovalbumin (OVA)-induced allergic rhinitis (AR) mouse model. Oral administration of BCE in a dose-independent manner regulated the balance of Th1/Th2/Treg cell differentiation in AR mice. Accordingly, BCE attenuated the expression of Th2-related cytokines such as IL-4, IL-5 and IL-13 in nasal lavage fluid (NALF) and nasal tissue and up-regulated the secretion of Th1/Treg cells including IL-10, IL-12 and IFN-[Formula: see text]. Also, BCE inhibited the formation and migration of eosinophils to the nasal mucosa and NALF, as well as suppressed CCL24, an eosinophil-specific chemoattractant in NALF. The levels of anti-OVA specific IgE and anti-OVA specific IgG1 were decreased, and as a result, the allergic response was attenuated by BCE via inhibiting mast cells accumulation in nasal mucosa and serum histamine release. The nasal allergy symptoms, nasal mucosal swelling, epithelial barrier disruption and mucus hyperplasia were obviously ameliorated. These results suggest that BCE may have therapeutic potential for treating allergic rhinitis through modulating the accumulation and activation of important leukocytes in the immune system such as Th1, Th2, Treg, eosinophils and mast cells.

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