Immunohistology of the nasal mucosa in seasonal allergic rhinitis: Increases in activated eosinophils and epithelial mast cells

1992 ◽  
Vol 89 (4) ◽  
pp. 877-883 ◽  
Author(s):  
A BENTLEY ◽  
M JACOBSON ◽  
V CUMBERWORTH ◽  
J BARKANS ◽  
R MOQBEL ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cells ◽  
Nasal Mucosa ◽  
Seasonal Allergic Rhinitis

scholarly journals Comparison of Intranasal Hypertonic Dead Sea Saline Spray and Intranasal Aqueous Triamcinolone Spray in Seasonal Allergic Rhinitis

2005 ◽  
Vol 84 (7) ◽  
pp. 426-430 ◽  
Author(s):  
Scott Cordray ◽  
Jim B. Harjo ◽  
Linda Miner
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Seasonal Allergic Rhinitis ◽  
Dead Sea ◽  
Control Group ◽  
Life Questionnaire ◽  
Intranasal Corticosteroids ◽  
Eye Symptoms ◽  
The Dead ◽  
Positive Effect

Intranasal corticosteroids are well known to be efficacious in the treatment of allergic rhinitis. Nasal irrigation with saline, including hypertonic saline, has long been recommended for the treatment of sinonasal disease, and it has been shown to have a positive effect on the physiology of the nasal mucosa. Until now, no study of the clinical efficacy of intranasal hypertonic Dead Sea saline as a monotherapy for seasonal allergic rhinitis has been reported. We conducted a prospective, randomized, single-blind, placebo-controlled comparison of intranasal hypertonic Dead Sea saline spray and intranasal aqueous triamcinolone spray in 15 patients with seasonal allergic rhinitis. Results were based on a 7-day regimen. Based on Rhinoconjunctivitis Quality of Life Questionnaire scores, clinically and statistically significant (p < 0.0001) improvements were seen in both active-treatment groups; as expected, the corticosteroid spray was the more effective of the two treatments. No significant improvement occurred in the control group. Our preliminary results not only confirm the efficacy of intranasal corticosteroid therapy in moderate-to-severe allergic rhinitis, they also suggest that the Dead Sea saline solution can be an effective alternative in mild-to-moderate allergic rhinitis, particularly with respect to nasal and eye symptoms. The hypertonicity of the Dead Sea solution may have a positive effect on the physiology of the nasal mucosa by improving mucociliary clearance. In addition, the dominant cation in the Dead Sea solution— magnesium— probably exerts anti-inflammatory effects on the nasal mucosa and on the systemic immune response.

Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis: investigation using a liquid-based method

2008 ◽  
Vol 122 (7) ◽  
pp. 700-706 ◽  
Author(s):  
T Chalastras ◽  
P Nicolopoulou-Stamati ◽  
E Patsouris ◽  
A Eleftheriadou ◽  
D Kandiloros ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Heat Shock ◽  
Substance P ◽  
Heat Shock Protein ◽  
Vasoactive Intestinal Peptide ◽  
Positive Reaction ◽  
Nasal Mucosa ◽  
Heat Shock Protein 70 ◽  
Seasonal Allergic Rhinitis ◽  
Chi Square

AbstractObjective:The aim of this study was to investigate expression of the neuropeptides substance P, vasoactive intestinal peptide and heat shock protein 70 in the nasal mucosa cells of patients with seasonal allergic rhinitis, in order to obtain more information on the pathophysiological and immunological role of these markers in allergic rhinitis.Material and methods:Nasal epithelium specimens obtained from 42 patients with allergic rhinitis were studied, using Shandon's Papspin liquid-based cytology method. Smears were immunostained with antibodies against substance P, vasoactive intestinal peptide and heat shock protein 70, and the results were correlated with the clinical features of seasonal allergic rhinitis.Results:A positive reaction for substance P, vasoactive intestinal peptide and heat shock protein 70 was observed in 73.8, 66.7 and 69.0 per cent of the allergic rhinitis mucosal smears, respectively. The Pearson chi-square test showed that 40.5 per cent of the immunostained smears had a positive reaction for one or two of the markers studied (i.e. substance P, vasoactive intestinal peptide or heat shock protein 70), and that 47.6 per cent of the smears had a positive reaction for all the markers (p < 0.0001).Conclusions:We found a high level of expression of substance P and vasoactive intestinal peptide in the nasal mucosa smears of patients suffering from allergic rhinitis. This indicates a role for these neuropeptides in the neuroregulation of immunity and hypersensivity in this disease. Furthermore, expression of heat shock protein 70 may contribute to the development of allergic rhinitis.

Preventive Effect of Bupleurum chinense on Nasal Inflammation via Suppressing T Helper Type 2, Eosinophil and Mast Cell Activation

2019 ◽  
Vol 47 (02) ◽  
pp. 405-421 ◽  
Author(s):  
Thi Tho Bui ◽  
Chun Hua Piao ◽  
Eunjin Hyeon ◽  
Yanjing Fan ◽  
Dae Woon Choi ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cells ◽  
Nasal Mucosa ◽  
Cell Activation ◽  
Therapeutic Potential ◽  
Specific Effect ◽  
Lavage Fluid ◽  
Mast Cell Activation ◽  
Nasal Allergy ◽  
Independent Manner

Bupleurum chinense is distributed in East Asia and has been used as a traditional herbal medicine for more than a thousand years. Though B. chinense has been reported to have immunomodulatory, anti-inflammatory, anti-oxidant, hepato-protective, antipyretic, analgesic and antifibrotic effects, its specific effect on allergic rhinitis disease has not been clarified. In this study, we investigated the anti-allergic and anti-inflammation effects of B. chinense extract (BCE) in an ovalbumin (OVA)-induced allergic rhinitis (AR) mouse model. Oral administration of BCE in a dose-independent manner regulated the balance of Th1/Th2/Treg cell differentiation in AR mice. Accordingly, BCE attenuated the expression of Th2-related cytokines such as IL-4, IL-5 and IL-13 in nasal lavage fluid (NALF) and nasal tissue and up-regulated the secretion of Th1/Treg cells including IL-10, IL-12 and IFN-[Formula: see text]. Also, BCE inhibited the formation and migration of eosinophils to the nasal mucosa and NALF, as well as suppressed CCL24, an eosinophil-specific chemoattractant in NALF. The levels of anti-OVA specific IgE and anti-OVA specific IgG1 were decreased, and as a result, the allergic response was attenuated by BCE via inhibiting mast cells accumulation in nasal mucosa and serum histamine release. The nasal allergy symptoms, nasal mucosal swelling, epithelial barrier disruption and mucus hyperplasia were obviously ameliorated. These results suggest that BCE may have therapeutic potential for treating allergic rhinitis through modulating the accumulation and activation of important leukocytes in the immune system such as Th1, Th2, Treg, eosinophils and mast cells.

Distribution of Two Types of Mast Cells in the Nasal Mucosa after Chemosurgical Treatment of Allergic Rhinitis using Trichloroacetic Acid

1999 ◽  
Vol 13 (2) ◽  
pp. 141-144 ◽  
Author(s):  
Kazuo Yao ◽  
Tatsutoshi Suzuki ◽  
Yoshiaki Iguchi ◽  
Kouichiro Nishiyama ◽  
Kazuhiro Yamamoto ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cells ◽  
Nasal Mucosa ◽  
Trichloroacetic Acid

Increase in epithelial mast cell numbers in the nasal mucosa of patients with perennial allergic rhinitis

1996 ◽  
Vol 110 (10) ◽  
pp. 929-933 ◽  
Author(s):  
A. Slater ◽  
L. A. Smallman ◽  
A. B. Drake-Lee
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cells ◽  
Light Microscopy ◽  
Nasal Mucosa ◽  
Inferior Turbinate ◽  
Control Group ◽  
Perennial Allergic Rhinitis ◽  
Immunohistochemical Technique ◽  
Cell Numbers ◽  
Staining Methods

AbstractThe aim of the study was to compare the numbers and distribution of mast cells in the nasal mucosa of perennial allergic rhinitis (PAR) patients and controls, as demonstrated by different staining methods for light microscopy.Biopsies of inferior turbinate mucosa were taken from 10 patients with PAR and 10 patients undergoing septoplasty or septorhinoplasty (control group). Sections for light microscopy were stained with azure A, chloroacetate esterase and an ABC immunohistochemical technique using antibody to tryptase.Three times more mast cells were found in the epithelium of PAR patients compared to controls using the immunohistochemical technique (p = 0.0074). This method demonstrated considerably more mast cells than the other stains.The increase in epithelial mast cells is consistent with the migration of mast cells seen in seasonal allergic rhinitis, and this may be important in the phenomenon of nasal priming seen after repeated antigen exposure.

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