scholarly journals Identification of Bacterial Profiles and Their Interactions with Selected Quality, Oxidative, and Immunological Parameters of Turkey Semen

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1771
Author(s):  
Michal Lenický ◽  
Tomáš Slanina ◽  
Miroslava Kačániová ◽  
Lucia Galovičová ◽  
Michaela Petrovičová ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Inflammatory Markers ◽  
Positive Relationship ◽  
Bacterial Load ◽  
Computer Assisted ◽  
Sperm Dna Fragmentation ◽  
Structural Deterioration ◽  
Acrosome Integrity

This study focused on the identification of naturally occurring bacteria in the reproductive fluid and impact on the quality of ejaculates obtained from the turkey breed British United Turkeys (BUT) Big 6 (n = 60). We determined possible relationships between the bacterial load and advanced sperm quality parameters that are important for effective artificial insemination and high fertility, as well as the concentration of selected antimicrobial proteins and pro-inflammatory markers of turkey semen. Sperm motility was assessed with computer-assisted sperm analysis (CASA), while the membrane and acrosome integrity were examined with smearing and staining methods. Reactive oxygen species (ROS) generation was quantified via luminometry, sperm DNA fragmentation was evaluated using the TUNEL assay, and the JC-1 assay was applied to evaluate the mitochondrial membrane potential. Cell lysates were prepared to investigate the extent of lipid and protein oxidation. Furthermore, levels of interleukins 1 and 6 (IL-1, IL-6), C-reactive protein, cathelicidin, and β-defensin were quantified in the seminal plasma using the ELISA method. The most dominant species identified by the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry was Escherichia coli, Proteus mirabilis, Staphylococcus lentus, and Citrobacter braakii. The bacterial load had a negative effect on the sperm motility (p < 0.001), as well as membrane (p < 0.05) and acrosome integrity (p < 0.01). A strong positive relationship between the bacterial load and DNA fragmentation (p < 0.001) was detected as well. Positive associations were recorded between the increasing presence of bacteria, ROS overgeneration (p < 0.001), and a subsequent oxidative damage to the proteins (p < 0.001) and lipids (p < 0.01). It was revealed that the antimicrobial peptides β-defensin (p < 0.001) and cathelicidin (p < 0.001) had a positive relationship with the motility. In contrast, pro-inflammatory markers, such as IL-1 (p < 0.001) and IL-6 (p < 0.001), had a negative impact on the motion behavior of turkey spermatozoa. Our results suggest that the semen quality may be notably affected by the bacterial quantity as well as quality. It seems that bacteriospermia is associated with inflammatory processes, oxidative stress, sperm structural deterioration, and a subsequent risk for a failed artificial insemination in turkey breeding.

Use of laptop computers connected to internet through Wi-Fi decreases human sperm motility and increases sperm DNA fragmentation

2012 ◽  
Vol 97 (1) ◽  
pp. 39-45.e2 ◽  
Author(s):  
Conrado Avendaño ◽  
Ariela Mata ◽  
César A. Sanchez Sarmiento ◽  
Gustavo F. Doncel
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Human Sperm ◽  
Sperm Dna Fragmentation ◽  
Laptop Computers ◽  
Sperm Dna

scholarly journals Functional Assessment of Diluent Choice for Semen Cryopreservation from Stallions with High and Low Freezability

2019 ◽  
Vol 47 (1) ◽  
Author(s):  
Heder Nunes Ferreira ◽  
José Carlos Ferreira-Silva ◽  
Jorge Motta Rocha ◽  
Pamela Ramos-Deus ◽  
Joane Isis Travassos Ribeiro ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Genetic Factors ◽  
Membrane Integrity ◽  
Sperm Dna Fragmentation ◽  
Fertility Rates ◽  
Livestock Species ◽  
Multiple Origins ◽  
Sperm Dna ◽  
Sperm Membrane

Background: fertility rates using horse frozen-thawed semen remain lower than in other livestock species. This fact further suggests that horse semen hold intrinsic sensitivity to cryoinjury that must be investigated. Moreover, there is a substantial influence of genetic factors and diluent choice upon horse cryopreservation outcome. Collectively, these genetic and technical properties of horse semen could be explored to identify factors or conditions that may increase semen viability after freeze-thawing. The aim of this work was to evaluate the effect of diluents Botu-Crio®,Lactose-EDTA®, and INRA-82® on cryopreserved semen from stallions with high (HFA) and low freezability (LFA).Materials, Methods & Results: frozen-thawed semen was evaluated for motility, membrane integrity, and sperm DNA fragmentation using the thermoresistance test (TRT). Comparisons for each parameter were done in a pair-wise fashion between HFA and LFA semen at one-hour intervals during the TRT (0 h - 4 h). Sperm motility in HFA, regardless of the diluent, was larger (P < 0.05) than LFA, both on 0h and 1h. In the 2h evaluation, sperm motility using Botu-Crio® and Lactose-EDTA® was greater (P < 0.05) for HFA. Analysis of sperm membrane integrity was similar between HFA and LFA semen (P > 0.05) at 0 h and 3 h. Sperm DNA fragmentation was lower (P < 0.05) in HFA semen at 0 h and 1 h. Discussion: frozen-thawed semen from stallions of high freezing ability showed greater motility at all analysis, irrespectively of diluent choice, suggesting a strong influence of genetic factors on cryopreservation outcome. Membrane integrity was similar immediately after thawing but did differ later on other TRT time-points, irrespectively of diluent choice. As observed for motility, it was expected that sperm cells of stallions of HFA would show higher membrane integrity than their LFA counterparts. Sperm DNA fragmentation was quite low for both groups, as described in horses. Surprisingly, sperm DNA fragmentation incidence was constant throughout the analysis for both HFA and LFA. It was initially envisioned that increased DNA fragmentation would be found in semen from LFA stallions, since it is caused by multiple origins such as genetic factors. In conclusion, the semen diluent affects horse sperm motility after thawing, particularly from stallions with lower semen freezability.

105 Increasing fertility of interspecific hybrid males using biotechnological approaches

2021 ◽  
Vol 33 (2) ◽  
pp. 159
Author(s):  
A. Vetokh ◽  
A. Tadzhieva ◽  
B. Iolchiev ◽  
N. Volkova ◽  
V. Bagirov
Keyword(s):  
Dna Fragmentation ◽  
Interspecific Hybrid ◽  
Semen Quality ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Differential Staining ◽  
Computer Assisted ◽  
Sperm Dna Fragmentation ◽  
Russian Science

The results of AI depend on many factors, with the quality of semen being one of the most important. Not all male hybrids can meet the requirements for semen quality, because they often have reduced fertility following cryopreservation. Thus, it is necessary to improve semen processing before use in AI. The aim of the study was to evaluate the effectiveness of using the “swim-up” flotation method to improve sperm quality of hybrid males of the Ovis genus. Semen from interspecific hybrid rams (1/4 Argali×3/4 Romanov, n=15; 1/8 Argali×7/8 Romanov, n=15) was freshly obtained, frozen–thawed, and processed by the swim-up method. Evaluation of sperm motility was determined using computer-assisted semen analysis. Statistical analysis was performed using SPSS vs.15.0 (ANOVA and t-test; SPSS Inc.). Semen was collected during the breeding season (October–December) via artificial vagina. Assessment of acrosome integrity was determined using differential staining with a Diachem diff-quick kit (NPF ABRIS+). The degree of sperm DNA fragmentation was determined using the acridine-orange test. The sperm freezing/thawing cycle was accompanied by sperm damage and an increase in the proportion of immobile sperm from 10 to 58%, with non-progressive movement increasing from 9 to 19.3%. The number of spermatozoa with abnormal morphology doubled, and the DNA fragmentation index increased from 16 to 26%. Use of the swim-up procedure allowed us to sort progressively motile spermatozoa. The content of progressively motile spermatozoa in the samples obtained from the supernatant was 86%, which was 2.3 times higher than in frozen–thawed sperm (P≤0,01). The obtained results show the effective use of the swim-up procedure to determine the quality of semen in hybrid rams. These studies were carried out with financial support from the Russian Science Foundation, grant No. 18-16-00079 and the Ministry of Science and Higher Education of the Russian Federation.

scholarly journals Decreased bull fertility: age-related changes in sperm motility and DNA fragmentation

2020 ◽  
Vol 151 ◽  
pp. 01010
Author(s):  
Berlin P. Pardede ◽  
Iman Supriatna ◽  
Yudi Yudi ◽  
Muhammad Agil
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Fertility Rate ◽  
Blue Staining ◽  
Age Group ◽  
Progressive Motility ◽  
Frozen Semen ◽  
Group 3 ◽  
Group 2

This study aimed to analyze the effect of the age of bulls on sperm motility and DNA fragmentation and its impact on fertility. Ninety-six frozen semen straw from eight bulls were divided into four groups based on age (group-1: 5-6 years; group-2: 7-8 years; group-3: 9-10 years; group-4: 11-12 years). Total and progressive motility were detected by using computer-assisted semen analysis (CASA), while DNA fragmentation was detected by Toluidine blue staining. Over 500 artificial insemination services in the field were used for fertility rate analysis. The results of the analysis of total motility, progressive, and DNA fragmentation in all age groups still meet the minimum standard for artificial insemination programs. Analysis of progressive motility and DNA fragmentation showed significant differences in each age group (P<0.01), whereas analysis of total motility showed no significant differences in group-2 (7-8 years) and group-3 (9-10 years) (P>0.01). Increased age in bulls correlated significantly with increased sperm DNA fragmentation (P<0.01), decreased total and progressive motility (P<0,01), and potentially reduced the fertility rate (P<0.01). In conclusion, although the quality of frozen semen still meets the standards for artificial insemination programs, the age factor in bulls needs to be considered for achieving maximum fertility.

scholarly journals Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Biology ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 300 ◽  
Author(s):  
Sara Sadeghi ◽  
Raquel Del Gallego ◽  
Balma García-Colomer ◽  
Ernesto A. Gómez ◽  
Jesús L. Yániz ◽  
...  
Keyword(s):  
Dilution Rate ◽  
Dna Fragmentation ◽  
Sperm Motility ◽  
Storage Temperature ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Effect Of Temperature ◽  
Sperm Dna Fragmentation ◽  
Liquid Storage ◽  
Duration Of Storage

The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of semen at different temperatures (5 °C or 17 °C), durations (0, 24 and 48 h) and sperm concentrations (250 × 106 sperm/mL (1:2 dilution rate), 166.7 × 106 sperm/mL (1:3 dilution rate) or 50 × 106 sperm/mL (1:10 dilution rate)) on sperm motility and kinetics were studied. In the second experiment, the effect of temperature, sperm washing and concentration on sperm motility and DNA fragmentation was studied. Finally, the effect of sperm concentration and duration of storage at 5 °C on sperm motility, response to oxidative stress and MMP was examined. We found that refrigerated liquid storage of goat sperm impaired sperm quality, such as motility, MMP and response to oxidation, as storage time increased; however, sperm DNA fragmentation index was not significantly affected. Liquid storage at 5 °C preserved higher total motility than at 17 °C. Moreover, we observed that the reduction of sperm concentration below 500 × 106 sperm/mL did not seem to improve the quality of spermatozoa conserved in milk-based extender in the conditions tested.

247 COMPARISON OF THE DNA FRAGMENTATION INDEX BETWEEN CRYOPRESERVED EJACULATED SPERM AND EPIDIDYMAL SPERM IN STALLIONS

2013 ◽  
Vol 25 (1) ◽  
pp. 271
Author(s):  
G. A. Monteiro ◽  
C. P. Freitas-DellAqua ◽  
P. N. Guasti ◽  
Y. F. R. Sancler-Silva ◽  
C. Ramires-Neto ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Epifluorescence Microscopy ◽  
Sperm Chromatin ◽  
Computer Assisted ◽  
Epididymal Sperm ◽  
Fragmentation Index ◽  
Motion Parameters ◽  
Motility Parameters ◽  
Dna Fragmentation Index

The development of a reliable technique for freezing epididymal semen would provide a unique opportunity to preserve valuable genetic material from unexpectedly lost stallions. The semen analysis method with the best ability to predict fertility is an examination of the sperm chromatin structure. This test evaluates the susceptibility of spermatozoa DNA to denaturation. The ability of spermatozoal DNA to maintain an intact double-stranded configuration is determined by exposure to an acid environment. The aim of this study was to compare the DNA fragmentation index of sperm obtained from ejaculate (G1) and sperm from the cauda epididymis (G2). For G1, two ejaculates from each of seven stallions were collected and then subjected to cryopreservation using BotuCrioTM extender. One week after the last semen collection, the stallions underwent bilateral orchiectomy. Sperm from the cauda epididymis was harvested immediately after castration (G2) by retrograde flushing of the caudal portion of the epididymis using a skim milk-based extender (BotuSemenTM). The recovered sperm was then cryopreserved using BotuCrioTM extender. The sperm motility parameters were analysed by computer-assisted sperm analysis (HTM IVOS 12, Hamilton Thorne Inc., Beverly, MA, USA), and the DNA fragmentation index was estimated using acridine orange test epifluorescence microscopy. The samples were evaluated immediately (0 h) and 8 h after thawing. The total motility, progressive motility, and percentage of rapid cells of the G1 v. G2 samples at 0 h were, respectively, 62.3 ± 12.9a v. 72.6 ± 8.4a, 31.6 ± 9.2a v. 35.3 ± 10.32a, and 49.3 ± 14.33a v. 59.7 ± 13.59a. At 8 h, the results were 26.0 ± 21.6b v. 54.7 ± 12.2a, 6.1 ± 6.4b v. 17.4 ± 8.54a, and 13.7 ± 14.85b v. 37.6 ± 14.15a. Evaluation of the DNA fragmentation in the G1 and G2 samples yielded 6.7 ± 1.41a v. 5.7 ± 1.60a at 0 h and 8.3 ± 1.78b v. 7.2 ± 1.19b at 8 h for percentage of DNA fragmentation after thawing. At 0 h, no differences in the sperm parameters were observed between groups, but statistical differences were observed in the sperm motility parameters between the treatment groups after 8 h. For the DNA fragmentation index, no difference was found at 0 and 8 h between the groups. However, after thawing, a higher percentage of DNA fragmentation was observed in the ejaculated sperm (8 h) as compared with the epididymal sperm (0 h). On the basis of these results, we can conclude that frozen–thawed cauda epididymal sperm had similar or higher motion parameters than ejaculated sperm after thawing. In addition, incubating the sperm at 20°C for 8 h after thawing resulted in higher motion parameters and less DNA fragmentation of the epididymal sperm. This finding suggests that epididymal sperm are more resistant to the cold shock caused by cryopreservation. FAPESP for financial support and Botupharma for donation of BotuSemenTM and BotuCrioTM extender.

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