scholarly journals Line Scan Spatial Speckle Contrast Imaging and Its Application in Blood Flow Imaging

2021 ◽  
Vol 11 (22) ◽  
pp. 10969
Author(s):  
E Du ◽  
Shuhao Shen ◽  
Anqi Qiu ◽  
Nanguang Chen
Keyword(s):  
Blood Flow ◽  
Laser Speckle ◽  
Experimental Results ◽  
Chick Embryos ◽  
Contrast Imaging ◽  
Laser Speckle Imaging ◽  
Speckle Imaging ◽  
Speckle Contrast ◽  
Line Scan

Laser speckle imaging has been an indispensable tool for visualizing blood flow in biomedical applications. We proposed a novel design of the laser speckle imaging system, which combines confocal illumination and detection with various speckle analysis methods. The system can be operated by three imaging modes. One is surface illumination laser speckle contrast imaging (SI-LSCI) and the other two are line scan temporal speckle contrast imaging (LS-TSCI) and line scan spatial speckle contrast imaging (LS-SSCI). The experimental results of flow phantoms have validated the mixture model, which combines the Lorentzian and Gaussian models to describe the simultaneous existence of both Brownian motions and ordered flow. Our experimental results of in vivo chick embryos demonstrate that LS-SSCI maintains high temporal resolution and is less affected by motion artifacts. LS-SSCI can provide better image quality for in vivo imaging blood chick embryos than LS-TSCI. Furthermore, the experiential results present that LS-SSCI can detect and quantify the blood flow change during vascular clipping, and shows great potential in diagnosing vascular diseases, such as angiosclerosis, angiostenosis, or angiemphraxis.

Laser Speckle Contrast Imaging on in vivo Blood Flow: a Review

2018 ◽  
Vol 45 (2) ◽  
pp. 0207006
Author(s):  
李晨曦 Li Chenxi ◽  
陈文亮 Chen Wenliang ◽  
蒋景英 Jiang Jingying ◽  
范颖 Fan Ying ◽  
杨婧孜 Yang Jingzi ◽  
...  
Keyword(s):  
Blood Flow ◽  
Laser Speckle ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast

scholarly journals A new simulation method for laser speckle imaging to investigate hemodynamics

2019 ◽  
Vol 128 ◽  
pp. 02001
Author(s):  
X Sang ◽  
D Li ◽  
B Chen
Keyword(s):  
Speckle Pattern ◽  
Simulation Method ◽  
Laser Speckle ◽  
Contrast Imaging ◽  
Laser Speckle Imaging ◽  
Speckle Imaging ◽  
Dynamic Speckle ◽  
Speckle Size ◽  
The One

Speckle simulation is a powerful protocol to investigate the properties of speckle and evaluate image processing method. However, only static speckle images can be simulated by available methods without considering time-integrated effect of CCD. A time–integrated dynamic speckle simulation method basedon coherent imaging was developed. Through the new simulation method, the effect of speckle size on LSCI was investigated. The smaller the speckle size is, the higher the spatial resolution become.But the one-dimensional speckle size should exceed two pixels to sample the speckle pattern. The characteristics of existing speckle contrast imaging methods were studied based on spatial statistics, and optimal parameters are given to obtain accurate and less noisy image. In general, the new simulation method for laser speckle imaging is a powerful tool to monitor blood flow in vivo and lay a solid foundation for the study of hemodynamics.

scholarly journals Improving sampling depth of laser speckle imaging by topical optical clearing: A theoretical and in vivo study

2019 ◽  
Vol 13 (02) ◽  
pp. 2050004
Author(s):  
D. Li ◽  
Y. Zhang ◽  
B. Chen
Keyword(s):  
Blood Flow ◽  
Penetration Depth ◽  
Laser Speckle ◽  
Skin Tissue ◽  
Laser Speckle Imaging ◽  
Sampling Depth ◽  
Speckle Imaging ◽  
Optical Clearing ◽  
Peg 400

The effect of optical cleaning method combined with laser speckle imaging (LSI) was discussed to improve the detection depth of LSI due to high scattering characteristics of skin, which limit its clinical application. A double-layer skin tissue model embedded with a single blood vessel was established, and the Monte Carlo method was used to simulate photon propagation under the action of light-permeating agent. 808[Formula: see text]nm semiconductor and 632.8[Formula: see text]nm He–Ne lasers were selected to study the effect of optical clearing agents (OCAs) on photon deposition in tissues. Results show that the photon energy deposition density in the epidermis increases with the amount of tissue fluid replaced by OCA. Compared with glucose solution, polyethylene glycol 400 (PEG 400) and glycerol can considerably increase the average penetration depth of photons in the skin tissue, thereby raising the sampling depth of the LSI. After the action of glycerol, PEG 400, and glucose, the average photon penetration depth is increased by 51.78%, 51.06%, and 21.51% for 808nm, 68.93%, 67.94%, and 26.67% for 632.8 nm lasers, respectively. In vivo experiment by dorsal skin chamber proves that glycerol can cause a substantial decrease in blood flow rate, whereas PEG 400 can significantly improve the capability of light penetration without affecting blood velocity, which exhibits considerable potential in the monitoring of blood flow in skin tissues.

scholarly journals Chronic Imaging of Cortical Blood Flow using Multi-Exposure Speckle Imaging

2013 ◽  
Vol 33 (6) ◽  
pp. 798-808 ◽  
Author(s):  
Syed Mohammad Shams Kazmi ◽  
Ashwin B Parthasarthy ◽  
Nelly E Song ◽  
Theresa A Jones ◽  
Andrew K Dunn
Keyword(s):  
Blood Flow ◽  
Vascular Remodeling ◽  
Laser Speckle ◽  
Contrast Imaging ◽  
Speckle Imaging ◽  
Speckle Contrast ◽  
Absolute Flow ◽  
Quantitative Accuracy ◽  
Flow Restoration ◽  
Velocity Changes

Chronic imaging of cerebral blood flow (CBF) is an important tool for investigating vascular remodeling after injury such as stroke. Although techniques such as Laser Speckle Contrast Imaging (LSCI) have emerged as valuable tools for imaging CBF in acute experiments, their utility for chronic measurements or cross-animal comparisons has been limited. Recently, an extension to LSCI called Multi-Exposure Speckle Imaging (MESI) was introduced that increases the quantitative accuracy of CBF images. In this paper, we show that estimates of chronic blood flow are better with MESI than with traditional LSCI. We evaluate the accuracy of the MESI flow estimates using red blood cell (RBC) photographic tracking as an absolute flow calibration in mice over several days. The flow measures computed using the MESI and LSCI techniques were found to be on average 10% and 24% deviant ( n = 9 mice), respectively, compared with RBC velocity changes. We also map CBF dynamics after photo-thrombosis of selected cortical microvasculature. Correlations of flow dynamics with RBC tracking were closer with MESI ( r = 0.88) than with LSCI ( r = 0.65) up to 2 weeks from baseline. With the increased quantitative accuracy, MESI can provide a platform for studying the efficacy of stroke therapies aimed at flow restoration.

scholarly journals Nephron blood flow dynamics measured by laser speckle contrast imaging

2011 ◽  
Vol 300 (2) ◽  
pp. F319-F329 ◽  
Author(s):  
Niels-Henrik Holstein-Rathlou ◽  
Olga V. Sosnovtseva ◽  
Alexey N. Pavlov ◽  
William A. Cupples ◽  
Charlotte Mehlin Sorensen ◽  
...  
Keyword(s):  
Blood Flow ◽  
Signal Transmission ◽  
Experimental Studies ◽  
Flow Dynamics ◽  
Laser Speckle ◽  
Tubuloglomerular Feedback ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Blood Flow Dynamics

Tubuloglomerular feedback (TGF) has an important role in autoregulation of renal blood flow and glomerular filtration rate (GFR). Because of the characteristics of signal transmission in the feedback loop, the TGF undergoes self-sustained oscillations in single-nephron blood flow, GFR, and tubular pressure and flow. Nephrons interact by exchanging electrical signals conducted electrotonically through cells of the vascular wall, leading to synchronization of the TGF-mediated oscillations. Experimental studies of these interactions have been limited to observations on two or at most three nephrons simultaneously. The interacting nephron fields are likely to be more extensive. We have turned to laser speckle contrast imaging to measure the blood flow dynamics of 50–100 nephrons simultaneously on the renal surface of anesthetized rats. We report the application of this method and describe analytic techniques for extracting the desired data and for examining them for evidence of nephron synchronization. Synchronized TGF oscillations were detected in pairs or triplets of nephrons. The amplitude and the frequency of the oscillations changed with time, as did the patterns of synchronization. Synchronization may take place among nephrons not immediately adjacent on the surface of the kidney.

Enhancing vascular visualization in laser speckle contrast imaging of blood flow using multi-focus image fusion

2018 ◽  
Vol 12 (1) ◽  
pp. e201800100 ◽  
Author(s):  
Wenzhi Lv ◽  
Yang Wang ◽  
Xiao Chen ◽  
Xiaoxi Fu ◽  
Jinling Lu ◽  
...  
Keyword(s):  
Blood Flow ◽  
Image Fusion ◽  
Laser Speckle ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Focus Image
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