scholarly journals Serpinin in the Skin

Biomedicines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 183
Author(s):  
Cristina Fraquelli ◽  
Jasmine Hauzinger ◽  
Christian Humpel ◽  
Maria Nolano ◽  
Vincenzo Provitera ◽  
...  
Keyword(s):  
Substance P ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Molecular Form ◽  
Proteolytic Processing ◽  
Sensory Nerves ◽  
Sensory Innervation ◽  
Autonomic Nerves ◽  
Rat Skin ◽  
Highly Active

The serpinins are relatively novel peptides generated by proteolytic processing of chromogranin A and they are comprised of free serpinin, serpinin-RRG and pGlu-serpinin. In this study, the presence and source of these peptides were studied in the skin. By Western blot analysis, a 40 kDa and a 50 kDa protein containing the sequence of serpinin were detected in the trigeminal ganglion and dorsal root ganglia in rats but none in the skin. RP-HPLC followed by EIA revealed that the three serpinins are present in similar, moderate amounts in rat dorsal root ganglia, whereas in the rat skin, free serpinin represents the predominant molecular form. There were abundant serpinin-positive cells in rat dorsal root ganglia and colocalization with substance P was evident. However, much more widespread distribution of the serpinins was found in dorsal root ganglia when compared with substance P. In the skin, serpinin immunoreactivity was found in sensory nerves and showed colocalization with substance P; as well, some was present in autonomic nerves. Thus, although not exclusively, there is evidence that serpinin is a constituent of the sensory innervation of the skin. The serpinins are biologically highly active and might therefore be of functional significance in the skin.

Membrane-associated microtubular areays induced in proximal myelinated processes of dorsal root ganglia by large doses of vitamin B6

1986 ◽  
Vol 44 ◽  
pp. 368-369
Author(s):  
V.J. Montpetit ◽  
S. Dancea ◽  
L. Tryphonas ◽  
D.F. Clapin
Keyword(s):  
Animal Model ◽  
Endoplasmic Reticulum ◽  
Dorsal Root Ganglia ◽  
Rough Endoplasmic Reticulum ◽  
Dorsal Root ◽  
Vitamin B6 ◽  
Morphological Changes ◽  
Model System ◽  
Sensory Nerves ◽  
Peripheral Sensory

Very large doses of pyridoxine (vitamin B6) are neurotoxic in humans, selectively affecting the peripheral sensory nerves. We have undertaken a study of the morphological and biochemical aspects of pyridoxine neurotoxicity in an animal model system. Early morphological changes in dorsal root ganglia (DRG) associated with pyridoxine megadoses include proliferation of neurofilaments, ribosomes, rough endoplasmic reticulum, and Golgi complexes. We present in this report evidence of the formation of unique aggregates of microtubules and membranes in the proximal processes of DRG which are induced by high levels of pyridoxine.

scholarly journals Renal sensory and sympathetic nerves reinnervate the kidney in a similar time-dependent fashion after renal denervation in rats

2013 ◽  
Vol 304 (8) ◽  
pp. R675-R682 ◽  
Author(s):  
Jan Mulder ◽  
Tomas Hökfelt ◽  
Mark M. Knuepfer ◽  
Ulla C. Kopp
Keyword(s):  
Substance P ◽  
Optical Density ◽  
Renal Denervation ◽  
Time Course ◽  
Sympathetic Nerves ◽  
Sensory Nerves ◽  
Sensory Innervation ◽  
Multiple Time ◽  
Sensory Reinnervation ◽  
Renal Sympathetic

Efferent renal sympathetic nerves reinnervate the kidney after renal denervation in animals and humans. Therefore, the long-term reduction in arterial pressure following renal denervation in drug-resistant hypertensive patients has been attributed to lack of afferent renal sensory reinnervation. However, afferent sensory reinnervation of any organ, including the kidney, is an understudied question. Therefore, we analyzed the time course of sympathetic and sensory reinnervation at multiple time points (1, 4, and 5 days and 1, 2, 3, 4, 6, 9, and 12 wk) after renal denervation in normal Sprague-Dawley rats. Sympathetic and sensory innervation in the innervated and contralateral denervated kidney was determined as optical density (ImageJ) of the sympathetic and sensory nerves identified by immunohistochemistry using antibodies against markers for sympathetic nerves [neuropeptide Y (NPY) and tyrosine hydroxylase (TH)] and sensory nerves [substance P and calcitonin gene-related peptide (CGRP)]. In denervated kidneys, the optical density of NPY-immunoreactive (ir) fibers in the renal cortex and substance P-ir fibers in the pelvic wall was 6, 39, and 100% and 8, 47, and 100%, respectively, of that in the contralateral innervated kidney at 4 days, 4 wk, and 12 wk after denervation. Linear regression analysis of the optical density of the ratio of the denervated/innervated kidney versus time yielded similar intercept and slope values for NPY-ir, TH-ir, substance P-ir, and CGRP-ir fibers (all R2 > 0.76). In conclusion, in normotensive rats, reinnervation of the renal sensory nerves occurs over the same time course as reinnervation of the renal sympathetic nerves, both being complete at 9 to 12 wk following renal denervation.

Distribution of substance P-like immunoreactivity in the spinal cord and dorsal root ganglia of the human foetus and infant

Neuroscience ◽  
1983 ◽  
Vol 10 (1) ◽  
pp. 41-55 ◽  
Author(s):  
Y. Charnay ◽  
C. Paulin ◽  
J.-A. Chayvialle ◽  
P.M. Dubois
Keyword(s):  
Spinal Cord ◽  
Substance P ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Human Foetus

scholarly journals Characteristics of Dorsal Root Ganglia Neurons Sensitive to Substance P

2014 ◽  
Vol 10 ◽  
pp. 1744-8069-10-73 ◽  
Author(s):  
Eder Ricardo Moraes ◽  
Christopher Kushmerick ◽  
Ligia Araujo Naves
Keyword(s):  
Substance P ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Dorsal Root Ganglia Neurons

Bradykinin-responsive cells of dorsal root ganglia in culture: Cell size, firing, cytosolic calcium, and substance P

1994 ◽  
Vol 14 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Masato Kano ◽  
Tadashi Kawakami ◽  
Naoshi Hikawa ◽  
Hideaki Hori ◽  
Toshifumi Takenaka ◽  
...  
Keyword(s):  
Substance P ◽  
Cell Size ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Cytosolic Calcium ◽  
Culture Cell

scholarly journals Sensation of Abdominal Pain Induced by Peritoneal Carcinomatosis Is Accompanied by Changes in the Expression of Substance P and μ-Opioid Receptors in the Spinal Cord of Mice

2012 ◽  
Vol 117 (4) ◽  
pp. 847-856 ◽  
Author(s):  
Masami Suzuki ◽  
Minoru Narita ◽  
Minami Hasegawa ◽  
Sadayoshi Furuta ◽  
Tomoyuki Kawamata ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Abdominal Pain ◽  
Substance P ◽  
Peritoneal Carcinomatosis ◽  
Opioid Receptor ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Receptor Expression ◽  
Tumor Bearing ◽  
Μ Opioid Receptor

Background Patients with peritoneal carcinomatosis often report abdominal pain, which is relatively refractory to morphine. It has been considered that a new animal model is required to investigate the mechanism of abdominal pain for the development of optimal treatments for this type of pain. Methods To prepare a peritoneal carcinomatosis model, highly peritoneal-seeding gastric cancer cells, 60As6, were implanted into the abdominal cavity. The nociceptive modality for pain-related behavior was assessed in terms of withdrawal behavior in response to mechanical stimuli and hunching behavior. Tissue samples from mouse dorsal root ganglia and spinal cord were subject to immunohistochemistry and real-time reverse transcription polymerase chain reaction. Results Mice with peritoneal dissemination showed significant hypersensitivity of the abdomen to mechanical stimulation and spontaneous visceral pain-related behavior. There was a significant increase in c-Fos-positive cells in the spinal cord in tumor-bearing mice. Those mice exhibited a remarkable increase in substance P-positive neurons in the dorsal root ganglia (control vs. tumor, 15.4 ± 1.1 vs. 24.2 ± 3.6, P < 0.05, n = 3). A significant decreases in μ-opioid receptor expression mainly in substance P-positive neurons was observed in tumor-bearing mice (69.3 ± 4.9 vs. 38.7 ± 0.9, P < 0.05, n = 3), and a relatively higher dose of morphine was required to significantly reverse the abdominal hypersensitivity. Conclusion Both the up-regulation of substance P and down-regulation of μ-opioid receptor seen in the dorsal root ganglia may be, at least in part, responsible for the abdominal pain-like state associated with peritoneal carcinomatosis.

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