scholarly journals An Electrochemical Enzyme Biosensor for Ammonium Detection in Aquaculture Using Screen-Printed Electrode Modified by Gold Nanoparticle/Polymethylene Blue

Biosensors ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 335
Author(s):  
Cong Wang ◽  
Tan Wang ◽  
Zhen Li ◽  
Xianbao Xu ◽  
Xiaoshuan Zhang ◽  
...  

A SPEC/AuNPs/PMB modified electrode was prepared by electrodeposition and electro-polymerization. The electrochemical behavior of reduced nicotinamide adenine dinucleotide (NADH) on the surface of the modified electrode was studied by cyclic voltammetry. A certain amount of substrate and glutamate dehydrogenase (GLDH) were coated on the modified electrode to form a functional enzyme membrane. The ammonia nitrogen in the water sample could be calculated indirectly by measuring the consumption of NADH in the reaction. The results showed that the strength of electro-catalytic current signal was increased by two times; the catalytic oxidation potential was shifted to the left by 0.5 V, and the anti-interference ability of the sensor was enhanced. The optimum substrate concentration and enzyme loading were determined as 1.3 mM NADH, 28 mM α-Ketoglutarate and 2.0 U GLDH, respectively. The homemade ceramic heating plate controlled the working electrode to work at 37 °C. A pH compensation algorithm based on piecewise linear interpolation could reduce the measurement error to less than 3.29 μM. The biosensor exhibited good linearity in the range of 0~300 μM with a detection limit of 0.65 μM NH4+. Compared with standard Nessler’s method, the recoveries were 93.71~105.92%. The biosensor was found to be stable for at least 14 days when refrigerated and sealed at 4 °C.

2021 ◽  
Vol 57 ◽  
pp. 128-141
Author(s):  
M. Ibrahim ◽  
V.G. Pimenov

A two-dimensional in space fractional diffusion equation with functional delay of a general form is considered. For this problem, the Crank-Nicolson method is constructed, based on shifted Grunwald-Letnikov formulas for approximating fractional derivatives with respect to each spatial variable and using piecewise linear interpolation of discrete history with continuation extrapolation to take into account the delay effect. The Douglas scheme is used to reduce the emerging high-dimensional system to tridiagonal systems. The residual of the method is investigated. To obtain the order of the method, we reduce the systems to constructions of the general difference scheme with heredity. A theorem on the second order of convergence of the method in time and space steps is proved. The results of numerical experiments are presented.


2021 ◽  
Vol 2021 (49) ◽  
pp. 37-44
Author(s):  
I. B. Ivasiv ◽  

It has been proposed to utilize the median algorithm for determination of the extrema positions of diffuse light reflectance intensity distribution by a discrete signal of a photodiode linear array. The algorithm formula has been deduced on the base of piecewise-linear interpolation for signal representation by cumulative function. It has been shown that this formula is much simpler for implementation than known centroid algorithm and the noise immune Blais and Rioux detector algorithm. Also, the methodical systematic errors for zero noise as well as the random errors for full common mode additive noises and uncorrelated noises have been estimated and compared for mentioned algorithms. In these terms, the proposed median algorithm is proportionate to Blais and Rioux algorithm and considerably better then centroid algorithm.


Author(s):  
Baoli Wang ◽  
Jiye Liang ◽  
Yuhua Qian ◽  
Chuangyin Dang

Decision makers often express their evaluations on decision problems with multi-granular linguistic terms. This fact leads to the unification of the multi-granular linguistic terms into a single linguistic set in the literature. However, this unification process increases the complexity of computation and the subjectivity in the determination of transformation functions. To overcome this deficiency, this paper aims to develop a normalized numerical scaling method for determining the semantics of multi-granular linguistic terms in the same domain. We first introduce a class of numerical scaling functions to generate several balanced or unbalanced linguistic sets. Since these scaled linguistic sets have different domains, we then develop a normalized numerical scaling method to form them into the unique interval [0,1]. As a result of this development, two classes of normalized scaling functions are derived from the priori scale information and applications of piecewise linear interpolation and piecewise arc interpolation. Finally, an example is given to illustrate how the method works.


2011 ◽  
Vol 271-273 ◽  
pp. 225-228
Author(s):  
Bai Hua Li ◽  
Lin Hua Piao ◽  
Ming Ming Ji

The method of interpolation was used in the process of software compensation of temperature in order to improve the environmental performance of air level posture sensor. Two algorithms including Lagrange interpolation and piecewise linear interpolation were calculated and compared in Matlab software, and then the optimization scheme could be achieved. The result showed that although the curse of Lagrange interpolation included all the given data positions, the Runge phenomenon in polynomial interpolation made the accuracy of interpolation lower. Piecewise linear interpolation reflected the relationship between environment and zero voltage more accurately. Piecewise linear interpolation not only can be used to improve the accuracy of software compensation of air level posture sensor.


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