scholarly journals Impact of Ethyl Methane Sulphonate Mutagenesis in Artemisia vulgaris L. under NaCl Stress

BioTech ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 18
Author(s):  
Sudheeran Pradeep Kumar ◽  
B.D. Ranjitha Kumari

The present investigation aimed to obtain salt-tolerant Artemisia vulgaris L. to develop a constant form through in vitro mutagenesis with ethyl methane sulphonate (EMS) as the chemical mutagen. NaCl tolerance was evaluated by the ability of the callus to maintain its growth under different concentrations, ranges from (0 mM to 500 mM). However, NaCl salinity concentration at (500 mM) did not show any development of callus, slight shrinking, and brown discoloration taking place over a week. Thus, all the biochemical and antioxidant assays were limited to (0–400 mM) NaCl. On the other hand, selected calluses were treated with 0.5% EMS for 30, 60, and 90 min and further subcultured on basal media fortified with different concentrations of 0–400 mM NaCl separately. Thus, the callus was treated for 60 min and was found to induce the mutation on the callus. The maximum salt-tolerant callus from 400 mM NaCl was regenerated in MS medium fortified with suitable hormones. Biochemical parameters such as chlorophyll, carotenoids, starch, amino acids, and phenol contents decreased under NaCl stress, whereas sugar and proline increased. Peroxidase (POD) and superoxide dismutase (SOD) activities peaked at 200 mM NaCl, whereas catalase (CAT) was maximum at 100 mM NaCl. Enhanced tolerance of 0.5% the EMS-treated callus, attributed to the increased biochemical and antioxidant activity over the control and NaCl stress. As a result, the mutants were more tolerant of salinity than the control plants.

2012 ◽  
Vol 11 (2) ◽  
pp. 104
Author(s):  
Yulmira Yanti ◽  
Trimurti Habazar ◽  
Mardinus ' ◽  
Mansyurdin '

The shoot of “rajasereh” banana was treated by 0,2% and 0,5% of ethyl methane sulfonate (EMS), for 2 and 4 hoursthourgh in vitro. The results showed that treatment of EMS mutagen would be changed morphologycal characterseither in planlet. In planlet stage was obtained four morphololgycal variations. One of the variant have characterizedthe colour yellowish; the leaves and stem are yellowish: the leaves were small and spiral. The value of coefficientof variant for morfphologycal characters of planlet “raja sereh” banana increased compare to the control. Theextreme value of coefficient of variant in planlet stage was found the times shoots is 84,31%, while control that is8,24%. EMS mutagen could caused several planlets shorter, total of leaves could of shoot are mostly


2012 ◽  
Vol 14 (1) ◽  
pp. 32
Author(s):  
Yulmira Yanti

The activity of peroxidase were observed in leave of banana clone Kepok resulting by treatments EMS. The phenotype of peroxidase wasanalyzed from the banana clones resulting the induced by EMS and without induction after inoculated with pathogen Banana Blood deasesBacterium (BBD). The objectives of research are know variation of activity and band pattern of peroxidase. Induced mutation treatmentsconsist of control, 0.2% EMS for 1 and 3 hours, 0.5% for 1 and 3 hours, each treatments was provided five banana clones. The result showedthat variant value and coefisien variant of peroxidase activity in leaf tissue of clone treated by EMS increased compare to the control. Avariant control is 0.28 with coefisien variant is 29.92%, while variant value of treatment is 8.45 with coefisien variant is 75.75%.Appearance of peroxidase bands on clone resulting by EMS treatments were emerged four band pattern. The first and control band patternhas relative migration distance is 20 and 30, the second 15, 40 and 60, the third is 15, 35 and 50. There are four bands with different relativemigration distance that indicated polymorphic.


2017 ◽  
Vol 9 (2) ◽  
pp. 20-27 ◽  
Author(s):  
Durre Shahwar ◽  
Mohammad Yunus Khali Ansari ◽  
Sana Chaudhary ◽  
Rumana Aslam

2016 ◽  
Vol 7 (1) ◽  
pp. 64
Author(s):  
Mahesh Kumar ◽  
Mukesh Kumar ◽  
Satya Prakash ◽  
DK Gautam ◽  
Sanjeev Rao

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