Overexpression of p62/IMP2 can Promote Cell Migration in Hepatocellular Carcinoma via Activation of the Wnt/β-Catenin Pathway

p62/IMP2 is an oncofetal protein that was first reported as a tumor-associated antigen in hepatocellular carcinoma (HCC). In our previous studies, we demonstrated a high frequency of p62/IMP2 autoantibodies appearing in various types of cancer. Therefore, we hypothesize that p62/IMP2 plays an important role in the progression of HCC, although the mechanism remains to be explored. In this study, we evaluated the expression of p62/IMP2 protein both in human tissues and liver cancer cell lines by immunohistochemistry and western blotting analysis and found that p62/IMP2 protein is overexpressed in human HCC tissue in comparison to normal human liver tissue. To explore the role that p62/IMP2 plays in HCC, p62/IMP2 was knocked out in two p62/IMP2-positive liver cancer cell lines (SNU449 and HepG2). Due to the low expression level of p62/IMP2 in SNU449, we overexpressed p62/IMP2 in this cell line. We subsequently demonstrated that high expression of p62/IMP2 in both cell lines can promote cell migration and invasion abilities in vitro by activating the Wnt/β-catenin pathway. We also used the Wnt/β-catenin pathway inhibitor, XAV 939, and a phosphoproteome assay to confirm our findings. Conclusion: Our results suggest that p62/IMP2 is an essential regulator of Wnt signaling pathways and plays an important role in HCC progression and metastasis.

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Anti-Proliferative Effects of an Extra-Virgin Olive Oil Extract Enriched in Ligstroside Aglycone and Oleocanthal on Human Liver Cancer Cell Lines

Cancers ◽

10.3390/cancers11111640 ◽

2019 ◽

Vol 11 (11) ◽

pp. 1640 ◽

Cited By ~ 5

Author(s):

De Stefanis ◽

Scimè ◽

Accomazzo ◽

Catti ◽

Occhipinti ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Cancer ◽

Olive Oil ◽

Cell Lines ◽

Cancer Cell ◽

Virgin Olive Oil ◽

Cancer Cell Lines ◽

Liver Cancer Cell ◽

Antioxidant Power ◽

Polyphenolic Extract

Oleocanthal and ligstroside aglycone are olive oil-derived polyphenols. The former interferes with tumor growth with minor or no cytotoxicity on non-tumorigenic primary cell lines. The information about the bioactivity of ligstroside aglycone are scanty, with the exception of a known antioxidant power. Hepatocellular carcinoma is a malignant tumor with high mortality rates. Systemic chemotherapy is only marginally effective and is frequently complicated by toxicity. Previous observations have shown that hepatocellular carcinoma cell lines become more sensitive to taxol when it is combined with Tumor Necrosis Factor α (TNFα). The present work aimed to assess the effects of a polyphenolic extract containing both oleocanthal and ligstroside aglycone on proliferation and/or death in three liver cancer cell lines (HepG2, Huh7 and Hep3B). The possibility to enhance such effect by the addition of TNFα was also investigated. Both cell proliferation and death were enhanced by the exposure to the polyphenolic extract. Such effect was associated with induction of autophagy and could be potentiated by TNFα. The presence of ligstroside aglycone in the extract lowered the oleocanthal concentration required for cytotoxicity. These results show for the first time that the effects of a polyphenol extract can be potentiated by TNFα and that modulation of autophagy likely account for these effects.

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Quercetin Suppresses Proliferation of Liver Cancer Cell Lines In Vitro

Anticancer Research ◽

10.21873/anticanres.14469 ◽

2020 ◽

Vol 40 (8) ◽

pp. 4695-4700 ◽

Cited By ~ 1

Author(s):

TORU HISAKA ◽

HISAMUNE SAKAI ◽

TOSHIHIRO SATO ◽

YUICHI GOTO ◽

YORIKO NOMURA ◽

...

Keyword(s):

Liver Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Liver Cancer Cell

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IN VITRO GROWTH INHIBITORY EFFECT OF ZnO NANOPARTICLES ON HUMAN LIVER CANCER CELL LINES (Huh7)

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i6.33486 ◽

2019 ◽

pp. 191-193

Author(s):

ANANTHALAKSHMI R ◽

XAVIER RAJARATHINAM SR ◽

Mohamed Sadiq A ◽

MOHAMED SADIQ A

Keyword(s):

Liver Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Zno Nanoparticles ◽

Huh7 Cell ◽

Cancer Cell Lines ◽

Inhibitory Effect ◽

Liver Cancer Cell ◽

Zno Nps

Objective: The objective of the study was to access the anticancer activity of the biosynthesized ZnO nanoparticles against Huh7 liver cancer cell lines. Methods: The study was carried in vitro using Huh7 cell lines. The ZnO nanoparticles (ZnO NPs) were synthesized using Luffa acutangula peel extract and subjected to characterization by X-ray powder diffraction and transmission electron microscopy. The Huh7 cell lines were treated with ZnO NPs and done 3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide assay. For live and dead assay, the cell lines treated with ZnO NPs were subjected to acridine orange/ethidium (AO/ET) bromide assay. Results: The ZnO NPs synthesized show spherical structure with 10–20 nm size. The 50% of Huh7 proliferation were inhibited at the concentration (IC50) of 40 μg/ml. The AO/ET assay shows compact nucleus and fine cytoplasmic morphology in control cells and apoptotic stage in treated cells Conclusion: This study suggests that ZnO NPs can be prepared in environment-friendly method using aqueous extract of L. acutangula and can be used in cancer treatment effectively.

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