scholarly journals Correlation between Autofluorescence Intensity and Histopathological Features in Non-Melanoma Skin Cancer: An Ex Vivo Study

Cancers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 3974
Author(s):  
Ilaria Giovannacci ◽  
Marco Meleti ◽  
Federico Garbarino ◽  
Anna Maria Cesinaro ◽  
Ema Mataca ◽  
...  

Non-melanoma skin cancer (NMSC) is the most common malignant tumor affecting fair-skinned people. Increasing incidence rates of NMSC have been reported worldwide, which is an important challenge in terms of public health management. Surgical excision with pre-operatively identified margins is one of the most common and effective treatment strategies. Incomplete tumor removal is associated with a very high risk of recurrence and re-excision. Biological tissues can absorb and re-emit specific light wave-lengths, detectable through spectrophotometric devices. Such a phenomenon is known as autofluorescence (AF). AF spectroscopy has been widely explored for non-invasive, early detection of NMSC as well as for evaluation of surgical margins before excision. Fluorescence-aided diagnosis is based on differences in spectral characteristics between healthy and neoplastic skin. Understanding the biological basis of such differences and correlating AF intensity to histological features could improve the diagnostic accuracy of skin fluorescence spectroscopy. The primary objective of the present pre-clinical ex vivo study is to investigate the correlation between the intensity of cutaneous AF and the histopathological features of NMSC. Ninety-eight lesions suggestive for NMSCs were radically excised from 75 patients (46 M; 29 F; mean age: 79 years). After removal, 115 specific reference points on lesions (“cases”; 59 on BBC, 53 on SCC and 3 on other lesions) and on peri-lesional healthy skin (controls; 115 healthy skin) were identified and marked through suture stitches. Such reference points were irradiated at 400–430 nm wavelength, and resulting emission AF spectra were acquired through spectrophotometry. For each case, AFIR (autofluorescence intensity ratio) was measured as the ratio between the number of photons emitted at a wavelength ranging between 450 and 700 nm (peak: 500 nm) in the healthy skin and that was captured in the pathological tissue. At the histological level, hyperkeratosis, neoangiogenesis, cellular atypia, epithelial thickening, fibrosis and elastosis were quantified by light microscopy and were assessed through a previously validated grading system. Statistical correlation between histologic variables and AFIR was calculated through linear regression. Spectrometric evaluation was performed on 230 (115 cases + 115 controls) reference points. The mean AFIR for BCC group was 4.5, while the mean AFIR for SCC group was 4.4 and the fluorescence peaks at 500 nm were approximately 4 times lower (hypo-fluorescent) in BCCs and in SCCs than in healthy skin. Histological variables significantly associated with alteration of AFIR were fibrosis and elastosis (p < 0.05), neoangiogenesis, hyperkeratosis and epithelial thickening. Cellular atypia was not significantly associated with alteration of AFIR. The intensity of fluorescence emission in neoplastic tissues was approximately 4 times lower than that in healthy tissues. Histopathological features such as hyperkeratosis, neoangiogenesis, fibrosis and elastosis are statistically associated with the decrease in AFIR. We hypothesize that such tissue alterations are among the possible biophysical and biochemical bases of difference in emission AF between neoplastic and healthy tissue. The results of the present evaluation highlighted the possible usefulness of autofluorescence as diagnostic, non-invasive and real-time tool for NMSCs.

2007 ◽  
Author(s):  
Marica B. Ericson ◽  
John Paoli ◽  
Carl Ljungblad ◽  
Adaocha Odu ◽  
Maria Smedh ◽  
...  

2017 ◽  
Vol 931 ◽  
pp. 012036 ◽  
Author(s):  
E Drakaki ◽  
IA Sianoudis ◽  
EN Zois ◽  
M Makropoulou ◽  
AA Serafetinides ◽  
...  

2012 ◽  
Vol 25 (05) ◽  
pp. 385-389 ◽  
Author(s):  
R. Tamburro ◽  
T. Nicetto ◽  
M. P. Kowaleski ◽  
M. Petazzoni

SummaryObjectives: To evaluate the acetabular ventroversion obtained with a modified triple pelvic osteotomy (2.5 PO) compared with that resulting from standard double pelvic osteotomy (DPO).Study design: Ex vivo study.Animals: Seven pelves obtained from skeletally mature dogs with a total body weight ranging from 26–41 kg were used.Methods: Unilateral DPO technique and dorsal ischial mono-cortical osteotomy were performed on every right hemipelvis. Angular ventral rotation was measured by determining the relative orientation of two Kirschner wires placed in the ilial wing and in the dorsal acetabular rim.Results: The mean angle of ventroversion was 9.5 ± 5.2 degrees for the DPO group (range 2.1–18.1) and 10.9 ± 4.8 degrees for the 2.5 PO group (range 4.1–19.5). The mean difference between the 2.5 PO and DPO was 1.5 ± 0.6 degrees (range 0.5–2.1).Conclusions: The 2.5 PO technique increased acetabular ventroversion versus DPO.


2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Bernardo Almeida Aguiar ◽  
Rafael Santos Reinaldo ◽  
Luciana Maria Arcanjo Frota ◽  
Mônica Sampaio do Vale ◽  
Bruno Carvalho de Vasconcelos

Although Root ZX is considered the gold standard electronic foramen locator (EFL), two variations of this device were launched, however without different operating mechanisms. This investigation aims to evaluate the precision of Root ZX (RZX), Root ZX II (RII), and Root ZX Mini (RM) EFLs. After access cavity preparation, 32 mandibular single rooted human premolars had their real length measured with the aid of a #15 K-type manual file under magnification (25x). Electronic measurements were performed by the devices in an alternate order until the apical foramen was reached (0.0). Each measurement was performed with adjusted file to the real length of the teeth and verified with a digital caliper. The accuracy of the EFLs was 68.8% (RZX), 65.8% (RII), and 68.8% (RM), considering ±0.5 mm as a margin of tolerance. The mean errors of the devices were0.37±0.25 mm (RZX),0.41±0.34 mm (RII), and0.32±0.28 mm (RM). ANOVA and Tukey test were applied to analyze the obtained data, which showed that there were no statistically significant differences among the locators (P>.05). It can be concluded that the three tested devices demonstrated precise measurements of the real length of the canal without performance differences among them.


2017 ◽  
Vol 74 (4) ◽  
pp. 309
Author(s):  
Cristiane Ferreira Alfenas ◽  
Inês de Fátima De Azevedo Jacinto Inojosa ◽  
Júlio César De Azevedo Carvalhal ◽  
Mariana Teixeira Maneschy Faria ◽  
Fernanda Freitas Lins ◽  
...  

Objective: the aim of this study was to compare the amount of apically extruded debris during the removal of root canal filling material using nickel-titanium rotary retreatment instruments and Hedström files. Material and Methods: sixty mandibular incisors with a single canal were instrumented and obturated by cold lateral compaction. For retreatment, specimens were randomly divided into four groups (n = 15): Hedström files with or without solvent; D-RaCe system and Mtwo retreatment system. Debris extruded apically during the removal of canal filling material was collected into preweighed Eppendorf tubes. The tubes were then stored in an incubator at 70°C for 5 days. The weight of the dry extruded debris was established by subtracting the preretreatment and postretreatment weight of the Eppendorf tubes for each group. The mean weights of extruded material were analyzed by one-way ANOVA. Results: the results showed that all retreatment techniques caused apical extrusion of debris, but no statistically significant difference was observed between groups (p>0.05). Conclusion: under the conditions of this study, all retreatment techniques caused apical debris extrusion.


Sign in / Sign up

Export Citation Format

Share Document