scholarly journals Lymphoma versus Carcinoma and Other Collaborations

Cells ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 174
Author(s):  
Karen Pulford
Keyword(s):  
Tumor Cells ◽  
Clinical Symptoms ◽  
Accurate Diagnosis ◽  
Clinical Staging ◽  
Research Career ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Tissue Sections ◽  
Poorly Differentiated ◽  
Formalin Fixed

David Mason started his research career at a time when lymphoma diagnosis was based primarily on cellular morphology, clinical symptoms and special cytochemical stains using formalin fixed tissue sections. There were occasions, however, where the morphology was unhelpful, such as in the case of anaplastic or poorly differentiated tumours, where a distinction between lymphoma and a non-haematopoietic tumour was often problematical. Accurate diagnosis became even more important with the developments in the clinical staging of lymphoma and the availability of more effective treatments. One way forward to improve diagnosis was to use immunohistochemistry to study the antigens expressed by the tumor cells.

Effects of antigen retrieval by microwave heating in formalin-fixed tissue sections on a broad panel of antibodies

1994 ◽  
Vol 102 (3) ◽  
pp. 165-172 ◽  
Author(s):  
R. von Wasielewski ◽  
M. Werner ◽  
M. Nolte ◽  
L. Wilkens ◽  
A. Georgii
Keyword(s):  
Microwave Heating ◽  
Antigen Retrieval ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Tissue Sections ◽  
Formalin Fixed

A Basis for Distinguishing Cultured Dendritic Cells and Macrophages in Cytospins and Fixed Sections

2005 ◽  
Vol 8 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Jukka Vakkila ◽  
Michael T. Lotze ◽  
Connie Riga ◽  
Ronald Jaffe
Keyword(s):  
Dendritic Cells ◽  
Cell Types ◽  
Fixed Tissue ◽  
Reliable Identification ◽  
Formalin Fixed Tissue ◽  
Tissue Sections ◽  
Hla Dr ◽  
Formalin Fixed

There is a burgeoning literature on the contrasting role of intratumoral dendritic cells (DCs) and tumor-associated macrophages, making reliable identification of both cell types in clinical and experimental tissue sections important. However, because these cell types are closely related and share several differentiation antigens, their absolute distinction in tissue sections is difficult. We differentiated DCs and macrophages from monocytes in vitro, prepared cytospins and paraffin-embedded sections of the various cell populations, and tested a variety of antibodies that purportedly recognize monocytes and DCs for their capacity to react and distinguish cells after conventional formalin fixation. Cultured DCs but not macrophages were detected by fascin, DC-LAMP, and CD83 with a predictable increase in staining that paralleled their maturation. Staining by CD1a was found on immature DCs but was weak and absent on mature DCs and macrophages, respectively. CD14 and CD163 were characteristic for macrophages and absent on DCs. CD68, HLA-DR, and S100 did not discriminate between DCs and macrophages. We conclude that antigens such as HLA-DR and S100 are not in themselves sufficient for identification of DCs in formalin-fixed tissue sections, but that additional macrophage-specific (CD14, CD163) and DC-specific (CD1a, CD83, fascin, DC-LAMP) antigens should be used to distinguish cell types from each other and to provide information on their state of maturation.

scholarly journals Tyramine Amplification Technique in Routine Immunohistochemistry

1997 ◽  
Vol 45 (11) ◽  
pp. 1455-1459 ◽  
Author(s):  
Reinhard von Wasielewski ◽  
Michael Mengel ◽  
Suzanne Gignac ◽  
Ludwig Wilkens ◽  
Martin Werner ◽  
...  
Keyword(s):  
Cost Reduction ◽  
Developmental Process ◽  
Point Of View ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Tissue Sections ◽  
Wide Range ◽  
Amplification Technique ◽  
First Time ◽  
Formalin Fixed

Signal amplification in immunohistochemistry via binding of biotinylated tyramine to proteins near the site of peroxidase-labeled antibodies is a promising new technique, but studies investigating a wide range of markers are lacking. The tyramine amplification technique (TAT) was investigated on 85 antibodies using a simple and fast protocol, and TAT results were compared to those obtained with conventional immunohistochemistry. Using TAT, most of the markers could be 5- to 50-fold further diluted and still showed identical staining results compared with standard stainings (maximal 500-fold). However, the variable reactivity of the different markers with TAT underlines the need for individual testing of every antibody to determine the optimal dilution. Some antibodies against cell adhesion molecules could be demonstrated for the first time in archival, formalin-fixed tissue sections. TAT, if carefully evaluated, offers a revolutionary improvement for modern immunostaining, either to increase sensitivity or primary antibody dilutions (cost reduction). From a methodological point of view, immunohistochemistry has not reached its limits by far and TAT is an important progressive step in this developmental process. (J Histochem Cytochem 45:1455–1459, 1997)

Improved detection of mycobacteria species in formalin-fixed tissue sections

2011 ◽  
Vol 59 (5) ◽  
pp. 993-1005 ◽  
Author(s):  
John S Pedersen ◽  
Iain Clarke ◽  
John Mills
Keyword(s):  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Tissue Sections ◽  
Formalin Fixed

Standardization of Immunohistochemistry Based on Antigen Retrieval Technique for Routine Formalin-fixed Tissue Sections

1998 ◽  
Vol 6 (2) ◽  
pp. 89-96 ◽  
Author(s):  
Shan-Rong Shi ◽  
Richard J. Cote ◽  
Benjaporn Chaiwun ◽  
Lillian L. Young ◽  
Yan Shi ◽  
...  
Keyword(s):  
Antigen Retrieval ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Tissue Sections ◽  
Retrieval Technique ◽  
Formalin Fixed
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