scholarly journals A Comparative Analysis of Oocyte Development in Mammals

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 1002 ◽  
Author(s):  
Rozenn Dalbies-Tran ◽  
Véronique Cadoret ◽  
Alice Desmarchais ◽  
Sébastien Elis ◽  
Virginie Maillard ◽  
...  
Keyword(s):  
Experimental Data ◽  
Lipid Metabolism ◽  
Comparative Analysis ◽  
Sexual Reproduction ◽  
Bone Morphogenetic Protein ◽  
Oocyte Development ◽  
Distinctive Features ◽  
Morphogenetic Protein ◽  
Evolutionarily Conserved ◽  
Bone Morphogenetic Protein 15

Sexual reproduction requires the fertilization of a female gamete after it has undergone optimal development. Various aspects of oocyte development and many molecular actors in this process are shared among mammals, but phylogeny and experimental data reveal species specificities. In this chapter, we will present these common and distinctive features with a focus on three points: the shaping of the oocyte transcriptome from evolutionarily conserved and rapidly evolving genes, the control of folliculogenesis and ovulation rate by oocyte-secreted Growth and Differentiation Factor 9 and Bone Morphogenetic Protein 15, and the importance of lipid metabolism.

scholarly journals Expression Profiles of the Progesterone Receptor, Cyclooxygenase-2, Growth Differentiation Factor 9, and Bone Morphogenetic Protein 15 Transcripts in the Canine Oviducts during the Oestrous Cycle

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 454
Author(s):  
Jaime Palomino ◽  
Javiera Flores ◽  
Georges Ramirez ◽  
Victor H. Parraguez ◽  
Monica De los Reyes
Keyword(s):  
Gene Expression ◽  
Bone Morphogenetic Protein ◽  
Cyclooxygenase 2 ◽  
Oestrous Cycle ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Morphogenetic Protein ◽  
Growth Differentiation Factor 9 ◽  
Bone Morphogenetic Protein 15 ◽  
Cox 2

The gene expression in the canine oviduct, where oocyte maturation, fertilization, and early embryonic development occur, is still elusive. This study determined the oviductal expression of (PR), cyclooxygenase-2 (COX-2), growth differentiation factor 9 (GDF-9), and bone morphogenetic protein 15 (BMP-15) during the canine oestrous cycle. Samples were collected from bitches at anoestrus (9), proestrus (7), oestrus (8), and dioestrus (11), after routine ovariohysterectomy and the ovarian surface structures and plasma progesterone concentration evaluated the physiological status of each donor. The oviductal cells were isolated and pooled. Total RNA was isolated, and gene expression was assessed by qPCR followed by analysis using the t-test and ANOVA. The PR mRNA increased (P < 0.05) from the anoestrus to dioestrus with the plasma progesterone concentration (r = 0.8). COX-2 mRNA expression was low in the anoestrus and proestrus, and negligible in the oestrus, while it was around 10-fold higher (P < 0.05) in the dioestrus. The GDF-9 mRNA was expressed during all phases of the oestrous cycle and was most abundant (P < 0.05) during oestrus phase. The BMP-15 mRNA decreased (P < 0.05) in the anoestrus and proestrus phases. Thus, the transcripts were differentially expressed in a stage-dependent manner, suggesting the importance of oestrous cycle regulation for successful reproduction in dogs.

scholarly journals PCR-RFLP of bone morphogenetic protein 15 (BMP15/FecX) gene as a candidate for prolificacy in sheep

2018 ◽  
Vol 6 (sup1) ◽  
pp. S68-S72 ◽  
Author(s):  
Hiam Nagdy ◽  
Karima Gh.M. Mahmoud ◽  
Mohamed M.M. Kandiel ◽  
Nermeen A. Helmy ◽  
Shawky S. Ibrahim ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Morphogenetic Protein ◽  
Pcr Rflp ◽  
Bone Morphogenetic Protein 15
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