scholarly journals Chemically Functionalized Water-Soluble Single-Walled Carbon Nanotubes Obstruct Vesicular/Plasmalemmal Recycling in Astrocytes Down-Stream of Calcium Ions

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1597
Author(s):  
Manoj K. Gottipati ◽  
Elena Bekyarova ◽  
Robert C. Haddon ◽  
Vladimir Parpura
Keyword(s):  
Carbon Nanotubes ◽  
Plasma Membrane ◽  
Polyethylene Glycol ◽  
Adenosine Triphosphate ◽  
Calcium Ions ◽  
Glutamate Release ◽  
Single Walled Carbon Nanotubes ◽  
Water Soluble ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

We used single-walled carbon nanotubes chemically functionalized with polyethylene glycol (SWCNT-PEG) to assess the effects of this nanomaterial on astrocytic endocytosis and exocytosis. We observed that the SWCNT-PEG do not affect the adenosine triphosphate (ATP)-evoked Ca2+ elevations in astrocytes but significantly reduce the Ca2+-dependent glutamate release. There was a significant decrease in the endocytic load of the recycling dye during constitutive and ATP-evoked recycling. Furthermore, SWCNT-PEG hampered ATP-evoked exocytotic release of the loaded recycling dye. Thus, by functionally obstructing evoked vesicular recycling, SWCNT-PEG reduced glutamate release from astrocytes via regulated exocytosis. These effects implicate SWCNT-PEG as a modulator of Ca2+-dependent exocytosis in astrocytes downstream of Ca2+, likely at the level of vesicle fusion with/pinching off the plasma membrane.

scholarly journals One-step preparation of water-soluble single-walled carbon nanotubes

2009 ◽  
Vol 255 (15) ◽  
pp. 7095-7099 ◽  
Author(s):  
Li Zhang ◽  
Qing-Qing Ni ◽  
Yaqin Fu ◽  
Toshiaki Natsuki
Keyword(s):  
Carbon Nanotubes ◽  
Single Walled Carbon Nanotubes ◽  
Water Soluble ◽  
Single Walled Carbon ◽  
One Step ◽  
Walled Carbon Nanotubes

Synthesis of Water-Soluble PEGylated Single-Walled Carbon Nanotubes

2006 ◽  
Vol 18 (25) ◽  
pp. 5864-5868 ◽  
Author(s):  
Jayanta Chattopadhyay ◽  
Felipe de Jesus Cortez ◽  
Soma Chakraborty ◽  
Nigel K. H. Slater ◽  
W. E. Billups
Keyword(s):  
Carbon Nanotubes ◽  
Single Walled Carbon Nanotubes ◽  
Water Soluble ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

Attachment of Functionalized Single-Walled Carbon Nanotubes (SWNTs) to Silicon Surfaces

2008 ◽  
Vol 8 (3) ◽  
pp. 1545-1550 ◽  
Author(s):  
Liling Zeng ◽  
Nancy Pattyn ◽  
Andrew R. Barron
Keyword(s):  
Carbon Nanotubes ◽  
Single Walled Carbon Nanotubes ◽  
Water Soluble ◽  
Silicon Surfaces ◽  
One Pot ◽  
Single Walled Carbon ◽  
Direct Fluorination ◽  
Assembled Monolayers ◽  
The One ◽  
Walled Carbon Nanotubes

Single-walled carbon nanotubes (SWNTs) were functionalized by direct fluorination and subsequent reaction with 6-aminohexanoic acid for water-soluble carboxylic acid functionalized SWNTs (AHA-SWNTs). Both of the compounds were used as precursors to attach SWNTs to APTES coated silicon surfaces. AHA-SWNTs in aqueous solution were reacted with APTES self-assembled monolayers (SAMs) with coupling reagents N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). The surface coverage is a function of concentration of AHA-SWNTs, solvent and coupling method. While for the fluorinated SWNTs (F-SWNTs), direct addition of F-SWNTs to preformed APTES SAMs at 90 °C shows essentially no reaction, in contrast to the one-pot reaction of F-SWNTs with APTES molecules in the presence of SWNTs on a silicon substrate. This reaction route provides a convenient method to attach SWNTs to silicon surfaces.

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