scholarly journals Early Warning of Biological Threats via Surface-Enhanced Raman Spectroscopy: A Case Study of Bacillus Spores

Challenges ◽  
2016 ◽  
Vol 7 (2) ◽  
pp. 24
Author(s):  
Antonia Lai ◽  
Salvatore Almaviva ◽  
Valeria Spizzichino ◽  
Domenico Luciani
Keyword(s):  
Raman Spectroscopy ◽  
Early Warning ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Bacillus Spores

Bacillus Spore Classification via Surface-Enhanced Raman Spectroscopy and Principal Component Analysis

2008 ◽  
Vol 62 (3) ◽  
pp. 267-272 ◽  
Author(s):  
J. Guicheteau ◽  
L. Argue ◽  
D. Emge ◽  
A. Hyre ◽  
M. Jacobson ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Raman Spectroscopy ◽  
Bacterial Species ◽  
Principal Component ◽  
Component Analysis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Signal ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Bacillus Spores

Surface-enhanced Raman spectroscopy (SERS) can provide rapid fingerprinting of biomaterial in a nondestructive manner. The adsorption of colloidal silver to biological material suppresses native biofluorescence while providing electromagnetic surface enhancement of the normal Raman signal. This work validates the applicability of qualitative SER spectroscopy for analysis of bacterial species by utilizing principal component analysis (PCA) to show discrimination of biological threat simulants, based upon multivariate statistical confidence limits bounding known data clusters. Gram-positive Bacillus spores ( Bacillus atrophaeus, Bacillus anthracis, and Bacillus thuringiensis) are investigated along with the Gram-negative bacterium Pantoea agglomerans.

Optimized Silver Film over Nanosphere Surfaces for the Biowarfare Agent Detection Based on Surface-Enhanced Raman Spectroscopy

2005 ◽  
Vol 876 ◽  
Author(s):  
Xiaoyu Zhang ◽  
Richard P. Van Duyne
Keyword(s):  
Raman Spectroscopy ◽  
Silver Film ◽  
Diffuse Reflectance Spectroscopy ◽  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Infectious Dose ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Biowarfare Agent ◽  
Bacillus Spores

AbstractThis work presents the rapid detection of Bacillus subtilis spores, harmless simulants for Bacillus anthracis, using surface-enhanced Raman spectroscopy (SERS) on silver film over nanosphere (AgFON) substrates. Calcium dipicolinate (CaDPA), a biomarker for bacillus spores, can be extracted effectively from spores with nitric acid and successfully detected by SERS. The highly tunable nature of AgFON optical properties was exploited to establish general optimization conditions. AgFON surfaces optimized for 750-nm laser excitation have been characterized by UV-vis diffuse reflectance spectroscopy. The SERS signal from extracted CaDPA was evaluated over the spore concentration range 10-15-10-12M to determine the adsorption capacity of the AgFON surface and the limit of detection (LOD). These sensing capabilities have been successfully transitioned to an inexpensive, portable Raman spectrometer. Using the extraction method and this field-portable instrument, the anthrax infectious dose of 104spores were detected with only a 5-second collection period on a one-month-old prefabricated AgFON substrate.

Detection of Bacillus spores within 15 minutes by surface-enhanced Raman spectroscopy

2012 ◽  
Author(s):  
Chetan Shende ◽  
Frank Inscore ◽  
Hermes Huang ◽  
Stuart Farquharson ◽  
Atanu Sengupta
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Bacillus Spores

scholarly journals Detection of Bacillus Spores by Surface-Enhanced Raman Spectroscopy

Bioterrorism ◽  
10.5772/31859 ◽  
2012 ◽  
Author(s):  
Stuart Farquharson ◽  
Chetan Shende ◽  
Alan Gift ◽  
Frank Inscore
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Bacillus Spores

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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