Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide
Keyword(s):
LAMP is an approach for isothermal nucleic acids diagnostics with increasing importance but suffers from the need of tedious systems design and optimization for every new target. Here, we describe an approach for its simplification based on a single nucleoside-5′-O-triphosphate (dNTP) that is covalently modified with a DNA strand. We found that the DNA-modified dNTP is a substrate for DNA polymerases in versatile primer extension reactions despite its size and that the incorporated DNA indeed serves as a target for selective LAMP analysis.
2017 ◽
Vol 121
(12)
◽
pp. 2594-2602
◽
Keyword(s):
Keyword(s):
2004 ◽
Vol 69
(4)
◽
pp. 715-747
◽
Keyword(s):
1997 ◽
Vol 272
(6)
◽
pp. L1174-L1180
◽
Keyword(s):
2018 ◽
Vol 115
(40)
◽
pp. 9992-9997
◽
2005 ◽
Vol 49
(8)
◽
pp. 3153-3162
◽
Keyword(s):
Keyword(s):