Impact of Cosmetic Lotions on Nanoparticle Penetration through ex Vivo C57BL/6 Hairless Mouse and Human Skin: A Comparison Study

Background: Electrochemotherapy is a novel treatment for cutaneous and subcutaneous tumors utilizing the combination of electroporation and chemotherapeutic agents. Since tumors have an increasing incidence nowadays as a result of environmental and genetic factors, electrochemotherapy could be a promising treatment for cancer patients. Objective: The aim of this article is to summarize the novel knowledge about the use of electroporation for antitumor treatments and to present a new application of electrochemotherapy with a well-known plant derived antitumor drug betulinic acid. For the review we have searched the databases of scientific and medical research to collect the available publications about the use of electrochemotherapy in the treatment of various types of cancer. Method: By the utilization of the available knowledge, we investigated the effect of electroporation on the penetration of a topically applied betulinic acid formulation into the skin by ex vivo Raman spectroscopy on hairless mouse skin. Results: Raman measurements have demonstrated that the penetration depth of betulinic acid can be remarkably ameliorated by the use of electroporation, so this protocol can be a possibility for the treatment of deeper localized cancer nodules. Furthermore, it proved the influence of various treatment times, since they caused different spatial distributions of the drug in the skin. Conclusion: The review demonstrates that electrochemotherapy is a promising tool to treat different kinds of tumors with high efficiency and with only a few moderate adverse effects. Moreover, it presents a non-invasive method to enhance the penetration of antitumor agents, which can offer novel prospects for antitumor therapies.

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The potential of a site-specific delivery of thiamine hydrochloride as a novel insect repellent exerting long-term protection on human skin: In-vitro, ex-vivo study and clinical assessment

Journal of Pharmaceutical Sciences ◽

10.1016/j.xphs.2021.07.017 ◽

2021 ◽

Author(s):

Mai El Halawany ◽

Randa Latif ◽

Alia Badawi

Keyword(s):

Human Skin ◽

Clinical Assessment ◽

Ex Vivo ◽

Thiamine Hydrochloride ◽

Insect Repellent ◽

Site Specific ◽

A Site ◽

Ex Vivo Study

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Impact of optical clearing on ex vivo human skin optical properties characterized by spatially resolved multimodal spectroscopy

Journal of Biophotonics ◽

10.1002/jbio.202100202 ◽

2021 ◽

Author(s):

Sergey M. Zaytsev ◽

Marine Amouroux ◽

Grégoire Khairallah ◽

Alexey N. Bashkatov ◽

Valery V. Tuchin ◽

...

Keyword(s):

Optical Properties ◽

Human Skin ◽

Ex Vivo ◽

Optical Clearing ◽

Spatially Resolved

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Iontophoretic delivery of lidocaine hydrochloride through ex-vivo human skin

Journal of Dermatological Treatment ◽

10.1080/09546634.2019.1589640 ◽

2019 ◽

Vol 31 (2) ◽

pp. 191-199 ◽

Cited By ~ 1

Author(s):

Roopa G. Manjunatha ◽

Rachna Prasad ◽

Sunil Sharma ◽

R.P. Narayan ◽

Veena Koul

Keyword(s):

Human Skin ◽

Ex Vivo ◽

Lidocaine Hydrochloride ◽

Iontophoretic Delivery

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Comparison of cutaneous bioavailability of cosmetic preparations containing caffeine or -tocopherol applied on human skin models or human skin ex vivo at finite doses

The Essential Stratum Corneum ◽

10.1201/b14296-22 ◽

2002 ◽

pp. 125-134

Author(s):

F Dreher ◽

F Fouchard ◽

C Patouillet ◽

M Andrian ◽

J-T Simonnet ◽

...

Keyword(s):

Human Skin ◽

Ex Vivo ◽

Human Skin Models

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Enhanced Transdermal Delivery of Pranoprofen Using a Thermo-Reversible Hydrogel Loaded with Lipid Nanocarriers for the Treatment of Local Inflammation

Pharmaceuticals ◽

10.3390/ph15010022 ◽

2021 ◽

Vol 15 (1) ◽

pp. 22

Author(s):

María Rincón ◽

Marcelle Silva-Abreu ◽

Lupe Carolina Espinoza ◽

Lilian Sosa ◽

Ana Cristina Calpena ◽

...

Keyword(s):

Human Skin ◽

Topical Application ◽

Topical Treatment ◽

Transdermal Delivery ◽

Ex Vivo ◽

Skin Permeation ◽

In Vitro Release ◽

Heterogeneous Structure ◽

Clinical Environment ◽

Local Skin

A biocompatible topical thermo-reversible hydrogel containing Pranoprofen (PF)-loaded nanostructured lipid carriers (NLCs) was studied as an innovative strategy for the topical treatment of skin inflammatory diseases. The PF-NLCs-F127 hydrogel was characterized physiochemically and short-time stability tests were carried out over 60 days. In vitro release and ex vivo human skin permeation studies were carried out in Franz diffusion cells. In addition, a cytotoxicity assay was studied using the HaCat cell line and in vivo tolerance study was performed in humans by evaluating the biomechanical properties. The anti-inflammatory effect of the PF-NLCs-F127 was evaluated in adult male Sprague Daw-ley® rats using a model of inflammation induced by the topical application of xylol for 1 h. The developed PF-NLCs-F127 exhibited a heterogeneous structure with spherical PF-NLCs in the hydrogel. Furthermore, a thermo-reversible behaviour was determined with a gelling temperature of 32.5 °C, being close to human cutaneous temperature and thus favouring the retention of PF. Furthermore, in the ex vivo study, the amount of PF retained and detected in human skin was high and no systemic effects were observed. The hydrogel was found to be non-cytotoxic, showing cell viability of around 95%. The PF-NLCs-F127 is shown to be well tolerated and no signs of irritancy or alterations of the skin’s biophysical properties were detected. The topical application of PF-NLCs-F127 hydrogel was shown to be efficient in an inflammatory animal model, preventing the loss of stratum corneum and reducing the presence of leukocyte infiltration. The results from this study confirm that the developed hydrogel is a suitable drug delivery carrier for the transdermal delivery of PF, improving its dermal retention, opening the possibility of using it as a promising candidate and safer alternative to topical treatment for local skin inflammation and indicating that it could be useful in the clinical environment.

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ID3024 Polymeric nanoparticles display bactericidal effect and selective fermentation for the treatment of acne vulgaris

Biomedical Research and Therapy ◽

10.15419/bmrat.v4is.244 ◽

2017 ◽

Vol 4 (S) ◽

pp. 34

Author(s):

Ming-Fa Hsieh

Keyword(s):

Human Skin ◽

Ex Vivo ◽

Polymeric Nanoparticles ◽

Acne Vulgaris ◽

Short Chain Fatty Acids ◽

Bactericidal Effect ◽

Skin Microbiome ◽

Poly Ethylene Glycol ◽

Effective Doses ◽

Poly Ethylene

The use of antibiotics in the treatment of acne in specific group (pregnant women) of patients can lead to serious complications. We have previously demonstrated that the nanoparticles made of block copolymers of poly (ethylene glycol) and poly(e-caprolactone) can inhibit the growth of Propionibacterium acnes (P. acnes), a bacterium highly associated with the progress of acne vulgaris in the human skin [Polymers 2016; 8, 321]. To reduce the amount of antibiotics used in the treatment of skin acne, we have further demonstrated that a bacterium in the human skin microbiome can utilize PEG-based polymers to produce various short-chain fatty acids (SCFAs) which suppressed the growth of P. acnes. PEG-based polymers were chosen as selective fermentation initiators which specifically induced the fermentation of the skin commensal bacterium but not P. acnes. Interestingly, PEG-based polymers can efficiently suppress the growth of P. acnes. An acne ex vivo explant was established by using acne biopsies collected from patients with acne vulgaris at the early and middle stages. The levels of pro-inflammatory interleukin (IL)-8 cytokine in early- and middle-staged acnes were significantly higher than those in healthy skins. Incubation of acne ex vivo explants with sucrose remarkably reduced the level of IL-8 and the number of P. acnes. Results from mouse studies revealed that PEG-based polymer functions as antibiotic adjuvants which can considerably reduce the effective doses of clindamycin, a clinically-used acne antibiotic

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