scholarly journals Marker-Assisted Selection of Trees with MALE STERILITY 1 in Cryptomeria japonica D. Don

Forests ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 734 ◽  
Author(s):  
Yoshinari Moriguchi ◽  
Saneyoshi Ueno ◽  
Yoichi Hasegawa ◽  
Takumi Tadama ◽  
Masahiro Watanabe ◽  
...  
Keyword(s):  
Male Sterility ◽  
Linkage Map ◽  
Marker Assisted Selection ◽  
Tree Breeding ◽  
Segregation Ratio ◽  
Linkage Maps ◽  
Male Sterile ◽  
Miyagi Prefecture ◽  
Time Required ◽  
Selection Of

The practical use of marker-assisted selection (MAS) is limited in conifers because of the difficulty with developing markers due to a rapid decrease in linkage disequilibrium, the limited genomic information available, and the diverse genetic backgrounds among the breeding material collections. First, in this study, two families were produced by artificial crossing between two male-sterile trees, ‘Shindai11’ and ‘Shindai12’, and a plus tree, ‘Suzu-2’ (Ms1/ms1) (S11-S and S12-S families, respectively). The segregation ratio between the male-sterile and male-fertile trees did not deviate significantly from the expected 1:1 ratio in either family. These results clearly suggested that the male-sterile gene of ‘Shindai11’ and ‘Shindai12’ is MALE STERILITY 1 (MS1). Since it is difficult to understand the relative positions of each marker, due to the lack of a linkage map which all the closely linked markers previously reported are mapped on, we constructed a partial linkage map of the region encompassing MS1 using the S11-S and S12-S families. For the S11-S and S12-S families, 19 and 18 markers were mapped onto the partial linkage maps of the MS1 region, respectively. There was collinearity (conserved gene order) between the two partial linkage maps. Two markers (CJt020762_ms1-1 and reCj19250_2335) were mapped to the same position as the MS1 locus on both maps. Of these markers, we used CJt020762 for the MAS in this study. According to the MAS results for 650 trees from six prefectures of Japan (603 trees from breeding materials and 47 trees from the Ishinomaki natural population), five trees in Niigata Prefecture and one tree in Yamagata Prefecture had heterozygous ms1-1, and three trees in Miyagi Prefecture had heterozygous ms1-2. The results obtained in this study suggested that ms1-1 and ms1-2 have different geographical distributions. Since MAS can be used effectively to reduce the labor and time required for selection of trees with a male-sterile gene, the research should help ensure that the quantity of breeding materials will increase to assist future tree-breeding efforts.

scholarly journals Marker-assisted selection of trees with MALE STERALITY 1 in Cryptomeria japonica D. Don

2020 ◽  
Author(s):  
Yoshinari Moriguchi ◽  
Saneyoshi Ueno ◽  
Yoichi Hasegawa ◽  
Takumi Tadama ◽  
Masahiro Watanabe ◽  
...  
Keyword(s):  
Marker Assisted Selection ◽  
Segregation Ratio ◽  
Linkage Maps ◽  
Genomic Information ◽  
Male Sterile ◽  
Miyagi Prefecture ◽  
Plus Tree ◽  
Conserved Gene ◽  
Time Required ◽  
Selection Of

AbstractPractical use of marker-assisted selection (MAS) is limited in conifers because of the difficulty with developing markers due to a rapid decrease in linkage disequilibrium, the limited genomic information available, and the diverse genetic backgrounds among breeding material collections. First, in this study, two families were produced by artificial crossing between two male-sterile trees, Shindai11 and Shindai12, and a plus tree, Suzu-2 (Ms1/ms1) (S11-S and S12-S families, respectively). The segregation ratio between male-sterile and male-fertile trees did not deviate significantly from the expected 1:1 ratio in either family. These results clearly suggested that the male-sterile gene of Shindai11 and Shindai12 is MALE STERALITY 1(MS1). Because some markers reported previously have not been linkage mapped, we constructed a partial linkage map of the region encompassing MS1 using the S11-S and S12-S families. For the S11-S and S12-S families, 19 and 18 markers were mapped onto the partial linkage maps of MS1 region, respectively. There was collinearity (conserved gene order) between the two partial linkage maps. Two markers (CJt020762_ms1-1 and reCj19250_2335) were mapped to the same position as the MS1 locus on both maps. Of these markers, we used CJt020762 for MAS in this study. According to the MAS results for 650 trees from six prefectures of Japan (603 trees from breeding materials and 47 trees from the Ishinomaki natural population), five trees in Niigata Prefecture and one tree in Yamagata Prefecture had heterozygous ms1-1, and three trees in Miyagi Prefecture had heterozygous ms1-2. The results obtained in this study suggested that there may be geographical hotspots for the ms1-1 and ms1-2 alleles. Because MAS can be used effectively to reduce the labor and time required for selection of trees with a male-sterile gene, the number of breeding materials should increase in the future.

scholarly journals Marker-assisted selection of maintainer lines within an onion tropical population

2010 ◽  
Vol 67 (2) ◽  
pp. 223-227 ◽  
Author(s):  
Carlos Antonio Fernandes Santos ◽  
Daniela Lopes Leite ◽  
Valter Rodrigues Oliveira ◽  
Marciene Amorim Rodrigues
Keyword(s):  
Random Field ◽  
Comparative Studies ◽  
Marker Assisted Selection ◽  
Male Sterile ◽  
Maintainer Line ◽  
Marker System ◽  
Hybrid Production ◽  
Low Latitudes ◽  
Light Green ◽  
Selection Of

The traditional onion (Allium cepa) hybrid production requires the development of maintainer and male sterile lines and also a pollinator line with good specific combination ability. We report the identification of maintainer and male-sterile onion lines within the Brazilian 'Baia Periforme' derived population, 'Alfa São Francisco', associating random field pairing of male-fertile plants with selected male-sterile plants and PCR-based marker system monitoring S, T and N-cytoplasms. Male-sterile plants produced flowers with light green anthers which were easily detected in the field. A frequency of 2.0% of male-sterile plants was estimated in the 'Alfa São Francisco' sampled population. Male-sterile plants produced the 5'cob-marker 180-bp and the orfA501-marker 473-bp fragments, suggesting the T-cytoplasm type, while the maintainer line produced only the 5'cob-marker 180-bp. These identified lines will be important to develop tropical onion hybrids well-adapted to Brazilian low latitudes and to future comparative studies with other onion cytoplasmic genic male sterility systems.

Molecular mapping of three male-sterile, female-fertile mutants and generation of a comprehensive map of all known male sterility genes in soybean

Genome ◽  
2014 ◽  
Vol 57 (3) ◽  
pp. 155-160 ◽  
Author(s):  
Yang Yang ◽  
Benjamin D. Speth ◽  
Napatsakorn Boonyoo ◽  
Eric Baumert ◽  
Taylor R. Atkinson ◽  
...  
Keyword(s):  
Linkage Group ◽  
Male Sterility ◽  
Genetic Linkage ◽  
Chromosome 2 ◽  
Linkage Maps ◽  
Male Sterile ◽  
Molecular Linkage Group ◽  
Sterile Female ◽  
Genetic Linkage Maps ◽  
Sterility Genes

In soybean, an environmentally stable male sterility system is vital for making hybrid seed production commercially viable. Eleven male-sterile, female-fertile mutants (ms1, ms2, ms3, ms4, ms5, ms6, ms7, ms8, ms9, msMOS, and msp) have been identified in soybean. Of these, eight (ms2, ms3, ms5, ms7, ms8, ms9, msMOS, and msp) have been mapped to soybean chromosomes. The objectives of this study were to (i) locate the ms1, ms4, and ms6 genes to soybean chromosomes; (ii) generate genetic linkage maps of the regions containing these genes; and (iii) develop a comprehensive map of all known male-sterile, female-fertile genes in soybean. The bulked segregant analysis technique was used to locate genes to soybean chromosomes. Microsatellite markers from the corresponding chromosomes were used on F2 populations to generate genetic linkage maps. The ms1 and ms6 genes were located on chromosome 13 (molecular linkage group F) and ms4 was present on chromosome 2 (molecular linkage group D1b). Molecular analyses revealed markers Satt516, BARCSOYSSR_02_1539, and AW186493 were located closest to ms1, ms4, and ms6, respectively. The ms1 and ms6 genes, although present on the same chromosome, were independently assorting with a genetic distance of 73.7 cM. Using information from this study and compiled information from previously published male sterility genes in soybean, a comprehensive genetic linkage map was generated. Eleven male sterility genes were present on seven soybean chromosomes. Four genes were present in two regions on chromosome 2 (molecular linkage group D1b) and two genes were present on chromosome 13 (molecular linkage group F).

scholarly journals Proposed research on social perception of marker-assisted selection and its role in the forests of British Columbia

2014 ◽  
Vol 90 (05) ◽  
pp. 666-669 ◽  
Author(s):  
Chelsea Nilausen ◽  
Nancy Gélinas ◽  
Gary Bull
Keyword(s):  
Marker Assisted Selection ◽  
Mountain Pine Beetle ◽  
Significant Contribution ◽  
Tree Breeding ◽  
Forest Industry ◽  
Major Player ◽  
Pine Beetle ◽  
Genomic Tool ◽  
The Us ◽  
Selection Of

The forest industry is a major player in the provincial economy, provides a significant contribution to government revenue, and accounts for 3% of British Columbia's GDP. However, with the reduction of housing starts in the US in 2006, the economic crisis of 2008, a steady decline in newsprint demand, and the Mountain Pine Beetle epidemic, the provincial and federal governments have searched for ways to help transform the forest industry through innovation, improved environmental performance, and new markets. One such investment has been in marker-assisted selection (MAS), which is a genomic-based biotechnological tool that allows desired traits to be flagged on the genome. Since MAS is a new genomic tool to the forest industry, it is necessary to survey silviculture stakeholders in BC on their perception of this resource to tree breeders, its perceived use, and the context for which it should be implemented. If it is a tool whose implementation is perceived positively, it would significantly reduce the cycle of the tree breeding process, as it allows for the early selection of genotypic traits. Moreover, it would allow tree breeders to more efficiently and accurately select for improved wood qualities, growth rates, and resistance to pests, diseases, and climate change.

scholarly journals CYTOPLASMIC MALE STERILITY IN BARLEY. XI. THE msm2 CYTOPLASM

Genetics ◽  
1982 ◽  
Vol 102 (2) ◽  
pp. 285-295
Author(s):  
H Ahokas
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Male Fertility ◽  
Fertility Restoration ◽  
Nuclear Gene ◽  
Restorer Gene ◽  
Male Sterile ◽  
Partial Restoration ◽  
Restoration Ability ◽  
Selection Of

ABSTRACT A new cytoplasmic male sterility in barley (Hordeum vulgare s.l.) is described and designated as msm2. The cytoplasm was derived from a selection of the wild progenitor of barley (H. vulgare ssp. spontaneum). This selection, 79BS14-3, originates from the Southern Coastal Plain of Israel. The selection 79BS14-3 has a normal spike fertility in Finland. When 79BS14-3 was crossed by cv. Adorra, the F1 displayed partial male fertility and progeny of recurrent backcrosses with cv. Adorra were completely male sterile. Evidently 79BS14-3 is a carrier of a recessive or semidominant restorer gene of fertility. The dominant restorer gene Rfm1a for another cytoplasmic male sterility, msm1, is also effective in msm2 cytoplasm. The different partial fertility restoration properties of msm2 and msm1 cause these cytoplasms to be regarded as being distinct. Seventy spontaneum accessions from Israel have been studied for their capacity to produce F1 restoration of male fertility both in msm1 and in msm2 cytoplasms with a cv. Adorra-like seed parent (nuclear gene) background. The msm2 cytoplasm shows partial restoration more commonly than msm1 in these F1 combinations. The mean restoration percentage per accession for msm2 is 28, and for msm1 4. Most of the F1 seed set differences of the two cytoplasms are statistically significant. When estimated with partially restored F1 combinations, msm2 cytoplasm appeared to be about 50 times more sensitive to the male fertility-promoting genes present in the spontaneum accessions. The spontaneum sample from Central and Western Negev, which has been found to be devoid of restoration ability in msm1 cytoplasm, had only low partial restoration ability in msm2 (mean 0.3%). The female fertility of msm2 appears normal. The new msm2 cytoplasm could be useful in producing hybrid barley.

Classification of environmentally induced genetic male sterile lines of rice based on their fertility responses to photoperiod and temperature

1996 ◽  
Vol 127 (2) ◽  
pp. 161-167 ◽  
Author(s):  
S. H. Cheng ◽  
H. M. Si ◽  
L. S. Zhuo ◽  
Z. X. Sun
Keyword(s):  
Male Sterility ◽  
Interaction Effect ◽  
Hybrid Rice ◽  
Male Sterile ◽  
Line System ◽  
Genetic Male Sterility ◽  
Different Types ◽  
Photoperiod Sensitive ◽  
Selection Of

SUMMARYThe use of environmentally induced genetic male sterile (EGMS) rice could alter the development of hybrid rice from a three-line system to a two-line system. It is critical for the utilization of EGMS rice to determine which are the main environmental factors influencing fertility changes. Fertility responses to photoperiod (P) and temperature (T) were studied in 101 EGMS rice lines under nine controlled regimes combining three photoperiods (15·0, 14·0 and 12·5 h)x three temperatures (30·1, 24·1 and 23·1 °C). According to the variance analysis of seed-setting data, 96% of the total EGMS lines studied could be divided into three types as follows: (1) photoperiod-sensitive genetic male sterility (PGMS) characterized statistically by significant (P < 0·05) P and P × T interaction effects but by a non-significant T effect on fertility, (2) thermosensitive genetic male sterility (TGMS) by a significant T effect, a non-significant P effect and by either a significant or a non-significant P × T interaction effect on fertility, and (3) photo-thermosensitive genetic male sterility (P-TGMS) by only a significant P × T interaction effect on fertility. Among the japonica EGMS lines studied, PGMS, TGMS and P-TGMS accounted for 32·3, 9·7 and 51·6%, respectively. However, among the indica EGMS lines, no PGMS lines were detected and most of them were TGMS or P-TGMS (61·4 and 35·7%, respectively). The results indicate that the selection of indica PGMS lines of rice might be very difficult. The availability of different types of EGMS rice in two-line system hybrid rice is evaluated and the selection of an ideal model of response to photoperiod and temperature for indica EGMS is discussed.

scholarly journals Somatic Embryogenesis and Plant Regeneration from Sugi (Japanese Cedar, Cryptomeria japonica D. Don, Cupressaceae) Seed Families by Marker Assisted Selection for the Male Sterility Allele ms1

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1029 ◽  
Author(s):  
Tsuyoshi E. Maruyama ◽  
Saneyoshi Ueno ◽  
Satoko Hirayama ◽  
Takumi Kaneeda ◽  
Yoshinari Moriguchi
Keyword(s):  
Somatic Embryogenesis ◽  
Male Sterility ◽  
Marker Assisted Selection ◽  
Cryptomeria Japonica ◽  
Public Health Problem ◽  
Efficient Production ◽  
Initial Growth ◽  
Male Sterile ◽  
Embryogenic Cell ◽  
Somatic Plants

One of the possible countermeasures for pollinosis caused by sugi (Cryptomeria japonica), a serious public health problem in Japan, is the use of male sterile plants (MSPs; pollen-free plants). However, the production efficiencies of MSPs raised by conventional methods are extremely poor, time consuming, and resulting in a high seedling cost. Here, we report the development of a novel technique for efficient production of MSPs, which combines marker-assisted selection (MAS) and somatic embryogenesis (SE). SE from four full sib seed families of sugi, carrying the male sterility gene MS1, was initiated using megagametophyte explants that originated from four seed collections taken at one-week intervals during the month of July 2017. Embryogenic cell lines (ECLs) were achieved in all families, with initiation rates varying from 0.6% to 59%. Somatic embryos were produced from genetic marker-selected male sterile ECLs on medium containing maltose, abscisic acid (ABA), polyethylene glycol (PEG), and activated charcoal (AC). Subsequently, high frequencies of germination and plant conversion (≥76%) were obtained on plant growth regulator-free medium. Regenerated plantlets were acclimatized successfully, and the initial growth of male sterile somatic plants was monitored in the field.

scholarly journals Application of LW7 marker for identification of progenies with male sterility gene in sweet sorghum population

2013 ◽  
Vol 13 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Darika Bunphan ◽  
Prasit Jaisil ◽  
Jirawat Sanitchon
Keyword(s):  
Male Sterility ◽  
Sweet Sorghum ◽  
Segregation Ratio ◽  
Male Sterile ◽  
Mendelian Segregation ◽  
Breeding Programs ◽  
Sterility Gene ◽  
The One ◽  
B Lines ◽  
Male Sterility Gene

The objectives of this study were to verify the use of LW7 marker in identifying maintainer lines (B-lines) and restorer lines (R-lines) in grain sorghum and sweet sorghum, and to identify B-lines in the F2, BC1F2 and BC2F2 generations. Twenty five accessions of sorghum were evaluated, and LW7 marker correctly identified accessions which presented male sterility gene (rf4) in Suphan Buri1 and 03B cultivars; moreover, these genotypes did not show 779 bp band. The cross between Suphan Buri1 and a male-sterile line (A-line) 03A resulted in a sterilized male, confirming the usefulness of the marker in breeding programs. B-lines in the F2, BC1F2 and BC2F2 generations were identified by LW7 marker. The segregation ratio of 3:1 for male fertility and male sterility in the progenies of the three generations supported the one-gene model of Mendelian segregation. The use of marker assisted selection was successful for line development of sweet sorghum with male sterility.

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