Microwave Assisted Extraction and Pressurized Liquid Extraction of Sulfated Polysaccharides from Fucus virsoides and Cystoseira barbata

Sulfated polysaccharide fucoidan isolated from brown algae shows a wide range of biological activities that are significantly dependent on its chemical composition, which is closely related to the applied technique and extraction parameters. Therefore, the objective of this study was to evaluate the influence of microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) parameters (solvent, temperature, time, and number of cycles) on the Fucus virsoides and Cystoseira barbata polysaccharide yield (%PS) and chemical composition (total sugar, fucose, and sulfate group). The optimal MAE parameters that resulted in the highest polysaccharide extraction from F. virsoides and C. barbata were 0.1 M H2SO4 for 10 min at 80 °C, while the optimal PLE parameters were 0.1 M H2SO4, for two cycles of 15 min at 140 °C. Furthermore, the %PS, chemical structure, molecular properties, and antioxidant activity of the F. virsoides and C. barbata polysaccharide extracts obtained with MAE, PLE, and conventional extraction (CE) performed under previously determinate optimal conditions were compared. PLE resulted in a significantly higher %PS from F. virsoides, while for C. barbata, a similar yield was achieved with CE and PLE, as well as CE and MAE, for both algae. Furthermore, the polysaccharides obtained using PLE had the highest polydispersity index, fucose, and sulfate group content, and the lowest uronic acid content; however their antioxidant activity was lower.

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Comparison of pressurized liquid extraction and microwave assisted extraction for the determination of organochlorine pesticides in vegetables

Talanta ◽

10.1016/j.talanta.2006.07.012 ◽

2007 ◽

Vol 71 (3) ◽

pp. 1345-1351 ◽

Cited By ~ 65

Author(s):

M. Barriada-Pereira ◽

M.J. González-Castro ◽

S. Muniategui-Lorenzo ◽

P. López-Mahía ◽

D. Prada-Rodríguez ◽

...

Keyword(s):

Organochlorine Pesticides ◽

Liquid Extraction ◽

Pressurized Liquid Extraction ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction

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Pressurized Liquid Extraction and Microwave-Assisted Extraction in the Determination of Organochlorine Pesticides in Fish Muscle Samples

Journal of AOAC International ◽

10.1093/jaoac/91.1.174 ◽

2008 ◽

Vol 91 (1) ◽

pp. 174-180 ◽

Cited By ~ 1

Author(s):

Mercedes Barriada-Pereira ◽

Iván Iglesias-García ◽

María J Gonzlez-Castro ◽

Soledad Muniategui-Lorenzo ◽

Purificación López-Maha ◽

...

Keyword(s):

Organochlorine Pesticides ◽

Fish Muscle ◽

Extraction Methods ◽

Liquid Extraction ◽

Pressurized Liquid Extraction ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Relative Standard

Abstract This paper describes a comparative study of 2 extraction methods, pressurized liquid extraction (PLE) and microwave-assisted extraction (MAE), for the determination of organochlorine pesticides (OCPs) in fish muscle samples. In both cases, samples were extracted with hexaneacetone (50 + 50), and the extracts were purified by solid-phase extraction using a carbon cartridge as the adsorbent. Pesticides were eluted with hexaneethyl acetate (80 + 20) and determined by gas chromatography with electron-capture detection. Both methods demonstrated good linearity over the range studied (0.0050.100 g/mL). Detection limits ranged from 0.029 to 0.295 mg/kg for PLE and from 0.003 to 0.054 mg/kg for MAE. For most of the pesticides, analytical recoveries with both methods were between 80 and 120, and the relative standard deviations were <10. The proposed methods were shown to be powerful techniques for the extraction of OCPs from fish muscle samples. Although good recovery rates were obtained with both extraction methods, MAE provided advantages with regard to sample handling, cost, analysis time, and solvent consumption. Acceptable validation parameters were obtained although MAE was shown to be more sensitive than PLE.

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