scholarly journals Optimisation of an Automated DNA Extraction Method for Bone and Teeth Samples and Applicability to Two Forensic Cases

2021 ◽  
Vol 1 (3) ◽  
pp. 194-201
Author(s):  
Jasmin Zoranjic ◽  
Jasmine W. Tay ◽  
Nicholas S. Mountford ◽  
Marie S. Rye
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Disaster Victim Identification ◽  
Victim Identification ◽  
Dna Quality ◽  
Dna Extraction Method ◽  
Organic Extraction ◽  
Automated Dna Extraction ◽  
Dna Profiles ◽  
Processing Steps

Bones and teeth are highly challenging sources of DNA in forensic science and human remains identification, requiring multiple laborious processing steps. In this study, we compared an organic phenol–chloroform method to the QIAamp® DNA Investigator and PrepFiler Express BTA™ methods in order to identify the most efficient automated DNA extraction method for bones and teeth. Results from individual tooth powder replicates showed that the PrepFiler Express BTA™ method extracted the highest yields of DNA per mg of tooth powder, returning a minimum of 20/21 PowerPlex® 21 loci. Samples extracted using the organic extraction or QIAamp® DNA Investigator methods produced PowerPlex® 21 profiles displaying a ski-slope morphology. The improved DNA quality and yield from the PrepFiler Express BTA™ method was verified using aged samples, where higher DNA yields per mg of powder and more informative profiles were obtained. Furthermore, the PrepFiler Express BTA™ method subsequently provided useful DNA profiles for two forensic cases involving degraded bone samples. Overall, this study showed that the PrepFiler Express BTA™ chemistry is a reliable and robust method for DNA extraction from bone and teeth samples, and will allow larger numbers of samples to be efficiently extracted in the event of a Disaster Victim Identification event.

Sensitivity detection of Abacavir in human through SNP detection of HLA-B*5701 allele

2016 ◽  
Vol 5 (08) ◽  
pp. 4754
Author(s):  
Tanushree Mitra* ◽  
Shivshankar Kumdale ◽  
Sameer Chowdhary ◽  
Amol D. Raut
Keyword(s):  
Blood Sample ◽  
Dna Extraction ◽  
Genomic Dna ◽  
Extraction Method ◽  
Snp Detection ◽  
Dna Extraction Method ◽  
Sensitivity Detection

The main objective of this study was to make sure whether randomly taken 12 samples were sensitive to abacavir. The genomic DNA from 12 blood sample were extracted by phenol chloroform DNA extraction method, extracted genomic DNA were amplified and sequenced, thereafter SNPs were detected. Every sample had shown the presence of normal base at SNP position. This study indicated, those randomly taken 12 patients were sensitive to abacavir, so they can consume abacavir if they get infected with HIV.

Development of a rapid DNA extraction method and one-step nested PCR for the detection of Naegleria fowleri from the environment

2011 ◽  
Vol 45 (16) ◽  
pp. 5211-5217 ◽  
Author(s):  
Arine Fadzlun Ahmad ◽  
James Lonnen ◽  
Peter W. Andrew ◽  
Simon Kilvington
Keyword(s):  
Dna Extraction ◽  
Nested Pcr ◽  
Extraction Method ◽  
Naegleria Fowleri ◽  
Dna Extraction Method ◽  
One Step ◽  
Nægleria Fowleri

scholarly journals An efficient DNA extraction method from bone and tooth samples by complete demineralization followed by the use of silica-based columns

2014 ◽  
Vol 23 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Md Mahamud Hasan ◽  
Tania Hossain ◽  
Ashish Kumar Majumder ◽  
Pilu Momtaz ◽  
Tarana Sharmin ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Extraction Method ◽  
Mechanical Grinding ◽  
High Concentration ◽  
Building Collapse ◽  
Dna Yield ◽  
Dna Extraction Method ◽  
Spin Column ◽  
Cryogenic Method ◽  
Dna Profiles

A highly efficient strategy for recovery of genomic DNA from bone and tooth samples is presented by complete demineralization of the bone pieces or intact tooth with high concentration of EDTA followed by spin column treatment without the need of mechanical grinding or cryogenic method for pulverizing the samples. The DNA yield was between 8 and 12 ng/?l from approximately 1 ?2 g of the starting material. Completed DNA profiles were obtained from of all the bones (52) and tooth (270) samples received from the unidentified victims from a recent building collapse, the Rana Plaza disaster in Dhaka, Bangladesh. DOI: http://dx.doi.org/10.3329/dujbs.v23i2.20089 Dhaka Univ. J. Biol. Sci. 23(2): 101-107, 2014

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