Stable DNA Aptamer–Metal–Organic Framework as Horseradish Peroxidase Mimic for Ultra-Sensitive Detection of Carcinoembryonic Antigen in Serum

Carcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. For CEA detection, a novel type of metal–organic framework (MOF) was prepared by grafting CEA aptamer-incorporated DNA tetrahedral (TDN) nanostructures into PCN-222 (Fe)-based MOF (referred as CEAapt-TDN-MOF colloid nanorods). The synthesized CEAapt-TDN-MOF is a very stable detection system due to the vertex phosphorylated TDN structure at the interface, possessing a one-year shelf-life. Moreover, it exhibits a significant horseradish peroxidase mimicking activity due to the iron porphyrin ring, which leads to a colorimetric reaction upon binding toward antibody-captured CEA. Using this method, we successfully achieved the highly specific and ultra-sensitive detection of CEA with a limit of detection as low as 3.3 pg/mL. In addition, this method can detect and analyze the target proteins in clinical serum samples, effectively identify the difference between normal individuals and patients with colon cancer, and provide a new method for the clinical diagnosis of tumors, demonstrating a great application potential.

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Highly Sensitive Detection of Trace Tetracycline in Water Using a Metal-Organic Framework-Enabled Sensor

Adsorption Science & Technology ◽

10.1155/2021/1462107 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Jiezeng Chen ◽

Hongying Shu ◽

Pingping Niu ◽

Pinghua Chen ◽

Hualin Jiang

Keyword(s):

Electrochemical Sensor ◽

Electrode Materials ◽

Limit Of Detection ◽

Metal Organic Framework ◽

Electrochemical Sensing ◽

Sensitive Detection ◽

Active Electrode ◽

Sensing Platform ◽

Metal Organic ◽

Organic Framework

Due to the abuse application of antibiotics in the recent decades, a high level of antibiotics has been let out and remains in our environment. Electrochemical sensing is a useful method to sensitively detect antibiotics, and the key factor for a successful electrochemical sensor is the active electrode materials. In this study, a sensitive electrochemical sensing platform based on a metal-organic framework (MOF) of MIL-53 (Fe) was facilely fabricated. It shows highly selective and sensitive detection performance for trace tetracycline. Differential pulse voltammetry (DPV) was applied to analyze the detection of tetracycline. The linear range of tetracycline detection was 0.0643 μmol/L-1.53 μmol/L, and the limit of detection (LOD) is 0.0260 μmol/L. Furthermore, the MOF-enabled sensor can be effectively used in actual water bodies. The results indicate that the electrochemical sensor is a high potential sensing platform for tetracycline.

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A bi-functionalized metal-organic framework based on N-methylation and Eu3+ post-synthetic modification for highly sensitive detection of 4-Aminophenol (4-AP), a biomarker for aniline in urine

Talanta ◽

10.1016/j.talanta.2021.122209 ◽

2021 ◽

Vol 227 ◽

pp. 122209

Author(s):

Yingmin Jin ◽

Bing Yan

Keyword(s):

Metal Organic Framework ◽

Sensitive Detection ◽

Highly Sensitive ◽

Metal Organic ◽

Highly Sensitive Detection ◽

Organic Framework

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Ionic Liquid-Functionalized Magnetic Metal–Organic Framework Nanocomposites for Efficient Extraction and Sensitive Detection of Fluoroquinolone Antibiotics in Environmental Water

ACS Applied Materials & Interfaces ◽

10.1021/acsami.0c17310 ◽

2021 ◽

Vol 13 (4) ◽

pp. 5357-5367

Author(s):

Dingkun Lu ◽

Menghan Qin ◽

Chang Liu ◽

Jingjing Deng ◽

Guoyue Shi ◽

...

Keyword(s):

Ionic Liquid ◽

Metal Organic Framework ◽

Environmental Water ◽

Sensitive Detection ◽

Fluoroquinolone Antibiotics ◽

Magnetic Metal ◽

Metal Organic ◽

Efficient Extraction ◽

Organic Framework

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Construction of Silver Quantum Dot Immobilized Zn-MOF-8 Composite for Electrochemical Sensing of 2,4-Dinitrotoluene

Applied Sciences ◽

10.3390/app9224952 ◽

2019 ◽

Vol 9 (22) ◽

pp. 4952 ◽

Cited By ~ 1

Author(s):

Sushma Rani ◽

Bharti Sharma ◽

Shivani Kapoor ◽

Rajesh Malhotra ◽

Rajender S. Varma ◽

...

Keyword(s):

Quantum Dot ◽

Limit Of Detection ◽

Metal Organic Framework ◽

Linear Sweep Voltammetry ◽

Electrochemical Sensing ◽

Amperometric Determination ◽

Transmission Electron ◽

Electro Catalysis ◽

Metal Organic ◽

Organic Framework

In the present study, we report a highly effective electrochemical sensor for detecting 2,4-dinitrotoluene (2,4-DNT). The amperometric determination of 2,4-DNT was carried out using a gold electrode modified with zinc–metal organic framework-8 and silver quantum dot (Zn-MOF-8@AgQDs) composite. The synthesized nanomaterials were characterized by using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRD). The synthesized nanocomposite proved to be efficient in electro-catalysis thereby reducing the 2,4-DNT. The unique combination present in Zn-MOF-8@AgQDs composite offered an excellent conductivity and large surface area enabling the fabrication of a highly sensitive (−0.238 µA µM−1 cm−2), selective, rapid and stable 2,4-DNT sensor. The dynamic linear range and limit of detection (LOD) was about 0.0002 µM to 0.9 µM and 0.041 µM, respectively. A 2,4-DNT reduction was also observed during the linear sweep voltammetry (LSV) experiments with reduction peaks at −0.49 V and −0.68 V. This is an unprecedented report with metal organic framework (MOF) composite for sensing 2,4-DNT. In addition, the presence of other species such as thiourea, urea, ammonia, glucose, and ascorbic acid displayed no interference in the modified electrode suggesting its practicability in various environmental applications.

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