scholarly journals Transcriptomic Profiling of Fruits from Pepper (Capsicum annuum L.), Variety Padrón (Mild Hot), at Two Ripening States

2021 ◽  
Vol 3 (1) ◽  
pp. 16
Author(s):  
Salvador González-Gordo ◽  
José M. Palma ◽  
Francisco J. Corpas
Keyword(s):  
Capsicum Annuum ◽  
Transcriptome Profiling ◽  
Rna Seq ◽  
Functional Categories ◽  
Transcriptomic Profiling ◽  
Local Conditions ◽  
Capsicum Annuum L ◽  
Climacteric Fruit ◽  
Genetic Level ◽  
Genus Capsicum

Pepper (Capsicum annuum L.) fruits are one of the most consumed vegetables worldwide. This produce has a great agro-economical relevance, since it is extensively cultivated. These fruits are characterized by their high content of vitamins C and A [1]. Capsicum annuum has many varieties, whose fruits differ in size, shape, color, and pungency, this last characteristic being due to the presence, in different degrees, of capsaicinoids and alkaloids, which are exclusive to the genus Capsicum [2]. The present study focuses on the transcriptomic profiling of an autochthonous Spanish variety called “Padrón” (mild hot) [3]. Pepper “Padrón” plants were grown in farms under the local conditions (42°44′05″ N 8°37′42″ W), and fruits at both green and red ripe ripening stages were collected. The transcriptome profiling was carried out in both types of fruits by RNA sequencing (RNA-seq) using the NextSeq550 system (Illumina®) [4,5]. RNA-seq analysis revealed that the expression of more than half of the 17,499 identified transcripts was modulated during ripening. Comparing to green fruits, 5626 and 5241 genes were up- and down-regulated, respectively, in red fruits. These differentially expressed genes (DEGs) have been analyzed to determine the functional categories that orchestrate the ripening process at the genetic level of this non-climacteric fruit.

scholarly journals Transcriptome profiling of abiotic responses to heat, cold, salt, and osmotic stress of Capsicum annuum L.

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Won-Hee Kang ◽  
Young Mi Sim ◽  
Namjin Koo ◽  
Jae-Young Nam ◽  
Junesung Lee ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Capsicum Annuum ◽  
Transcriptome Profiling ◽  
Capsicum Annuum L ◽  
Salt And Osmotic Stress

scholarly journals Integrative RNA-Seq analysis of Capsicum annuum L.-Phytophthora capsici L. pathosystem reveals molecular cross-talk and activation of host defence response

2021 ◽  
Author(s):  
Tilahun Rabuma ◽  
Om Prakash Gupta ◽  
Vinod Chhokar X ◽  
Manju Yadav X
Keyword(s):  
Capsicum Annuum ◽  
Molecular Mechanisms ◽  
Phytophthora Capsici ◽  
Enrichment Analysis ◽  
Defence Response ◽  
Chili Pepper ◽  
Rna Seq ◽  
Kegg Pathways ◽  
Capsicum Annuum L ◽  
Chili Peppers

Background: Chili pepper (Capsicum annuum L.) being one of an important member of the Solanaceae family, and its productivity is highly affected by the fungal pathogen Phytophthora capsici L. Other to CM-344, the unavailability of resistant landraces to all possible strains of P. capsici imposes a serious threat to its global production. This is because of our current understanding of the molecular mechanisms associated with the defence response in C. annuum-P. capsici pathosystem is limited. Therefore, the current study used RNA-seq technology to dissect the genes associated with defence response against P. capsici infection in two contrasting landraces, i.e. GojamMecha_9086 (Resistant) and Dabat_80045 (susceptible) exposed to P. capsici infection. Results: The transcriptomes from 4 leaf samples (RC, RI, SC and SI) of chili pepper resulted in a total of 1,18, 879 assembled transcripts (with a mean TL of 813.23bp and N50 of 1,277bp) along with 52,384 pooled unigenes with (mean UL of 1029.36 bp and N50 of 1,403bp). The enrichment analysis of the transcripts indicated 23 different KEGG pathways under five main categories. Further, 774 and 484 differentially expressed genes (DEGs) were obtained from RC vs. RI and SC vs. SI leaf samples, respectively. Of these, 57 DEGs were found to be associated with defence responses against P. capsici infection. The defence-related genes, such as LTPL, defensin J1-2-like, peroxidase 5-like, UGT, and GRP proteins-like, were more significantly upregulated in RC vs. RI. Furthermore, RT-qPCR analysis of six randomly selected genes validated the results of Illumina NextSeq500 sequencing results. Furthermore, a total of 58 TF families (bHLH most abundant) and 2,095 protein families (Protein kinase, PF00069, most abundant) were observed across all the samples with maximum hits in RI and SI samples. Conclusions: RNA-Seq analysis of chili peppers during P.capsici infection revealed differential regulation of genes associated with defence and signaling response with shared coordination of molecular function, cellular component and biological processing. The results presented here would enhance our present understanding of the defence response in chili pepper against P. capsici infection, which could be utilized by the molecular breeders to develop resistant chili genotypes. Keywords: Capsicum annuum, Phytophthora capsici, transcriptome sequence, differential gene expression

Pendugaan Parameter Genetik dan Seleksi Karakter Kuantitatif Cabai Rawit (Capsicum annuum L.) Populasi F3

2017 ◽  
Vol 5 (2) ◽  
pp. 213
Author(s):  
Abdul Rahman Fajar Sidiq ◽  
Muhamad Syukur ◽  
Siti Marwiyah
Keyword(s):  
Capsicum Annuum ◽  
Capsicum Annuum L

<em>Konsumsi cabai rawit terus meningkat namun produktivitasnya masih rendah, sehingga diperlukan pemuliaan yang mengarah pada perbaikan daya hasil. Penelitian ini bertujuan untuk menganalisis keragaman genetik, korelasi antar karakter, heritabilitas arti luas, dan nilai kemajuan seleksi populasi F3 cabai rawit. Penelitian ini dilaksanakan di Kebun Percobaan Leuwikopo, IPB, Dramaga, Bogor dan Laboratorium Genetika dan Pemuliaan Tanaman, Departemen Agronomi dan Hortikultura, IPB. Percobaan menggunakan 5 genotipe tetua dan 6 genotipe F3 cabai rawit, dengan genotipe tetua yang diulang sebanyak 3 ulangan dan genotipe F3 tanpa ulangan. Hasil penelitian menunjukkan bahwa karakter bobot buah per tanaman dan jumlah buah per tanaman memiliki keragaman genetik luas pada semua genotipe. Semua karakter pengamatan berkorelasi positif terhadap daya hasil kecuali umur berbunga dan umur panen. Nilai heritabilitas arti luas dengan kriteria tinggi terdapat pada karakter bobot buah per tanaman, bobot per buah, dan panjang buah. Hasil seleksi indeks yang dilakukan menunjukkan kemajuan seleksi yang besar pada karakter bobot buah per tanaman dan jumlah buah per tanaman.</em>

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