Transcriptome profiling of genes involved in nutrient uptake regulated by phosphate-solubilizing bacteria in pepper (Capsicum annuum L.)

2020 ◽  
Vol 156 ◽  
pp. 611-626
Author(s):  
Jian Zhang ◽  
Tingting Guo ◽  
Zhen Tao ◽  
Pengcheng Wang ◽  
Hongmei Tian
2021 ◽  
Vol 3 (1) ◽  
pp. 16
Author(s):  
Salvador González-Gordo ◽  
José M. Palma ◽  
Francisco J. Corpas

Pepper (Capsicum annuum L.) fruits are one of the most consumed vegetables worldwide. This produce has a great agro-economical relevance, since it is extensively cultivated. These fruits are characterized by their high content of vitamins C and A [1]. Capsicum annuum has many varieties, whose fruits differ in size, shape, color, and pungency, this last characteristic being due to the presence, in different degrees, of capsaicinoids and alkaloids, which are exclusive to the genus Capsicum [2]. The present study focuses on the transcriptomic profiling of an autochthonous Spanish variety called “Padrón” (mild hot) [3]. Pepper “Padrón” plants were grown in farms under the local conditions (42°44′05″ N 8°37′42″ W), and fruits at both green and red ripe ripening stages were collected. The transcriptome profiling was carried out in both types of fruits by RNA sequencing (RNA-seq) using the NextSeq550 system (Illumina®) [4,5]. RNA-seq analysis revealed that the expression of more than half of the 17,499 identified transcripts was modulated during ripening. Comparing to green fruits, 5626 and 5241 genes were up- and down-regulated, respectively, in red fruits. These differentially expressed genes (DEGs) have been analyzed to determine the functional categories that orchestrate the ripening process at the genetic level of this non-climacteric fruit.


2005 ◽  
Vol 7 (2) ◽  
pp. 41-47
Author(s):  
Aidi Noor

The aims of the experiment were to evaluate the effect of rock phosphate application and combination betweenphosphate-solubilizing bacteria with farm yard manure on nutrient uptake and yield of soybean. Factorial experiment design with two factors was used in randomized complzte block design with three replications. The first factors was level of rockphosphate i.e. : 0; 30; 60; 90 kg P ha· I, and the second factor was combination of phosphate solubili=ing bacteria andfarm yard manure: without phosphate solubi/i=ing bacteria andfarm yard manure; phosphate solubilbing bacteria (Pseudomonas fluorescens); farm yard manure 10 ton ha"I, and phosphate solubilizing bacteria + farm yard manure. The results indicated that rock phosphate and combination of phosphate solubilizing bacteria andfarm yard manure application increased nutrient (N, p, K) uptake and grain yield of soybean. Optimum dosage of rock phosphate in soil without phosphate-solubilizing bacteria andfarm yard manure (control) was 72.15 kg P hdl which gave maximum yield of soybean was 7.73 g pori. While with phosphate-solubilizing bacteria. farm yard manure and phosphate solubilizing bacteria+farm yard manure obtained optimum dosage of rock phosphate were 62.26, 63.94, and 62. 21 kg P hd1 , respectively, which gave maximum yield of soybean were 8.17, 7.95, and 8.43 g pori, respectively.


Author(s):  
G. Chandramohan Reddy ◽  
S.S. Hebbar ◽  
A.K. Nair ◽  
H.B. Raghupathy ◽  
A.P. Mallikarjuna Gowda ◽  
...  

Author(s):  
V.Z. Nguyen ◽  
T.O. Dao ◽  
T.H. Nguyen ◽  
E.A. Kalashnikova

Перец овощной (Capsicum annuum L.) является одной из основных возделываемых культур Вьетнама в связи с широким его использованием в пищевой промышленности в качестве приправы. Это делает данное растение важной сельскохозяйственной культурой, которая приносит значительный доход вьетнамским фермерам. Однако на плантациях перца часто наблюдается появление и развитие болезни ризоктониоз, которую вызывает патогенный гриб Rhizoctonia solani, а также наблюдается бактериальное увядание, вызываемое бактерией Ralstonia solanacearum. Поражение ризоктониозом, одной из самых вредных болезней перца, может наблюдаться в любой фазе развития растений. Известно, что ризобактерии способны не только стимулировать рост растений, но и подавлять развитие болезней. Цель данной работы выделение из ризосферы растений перцев бактерий, ингибирующих развитие Rhizoctonia solani и оценка in vitro их фосфатрастворяющей активности и секреции сидерофора. Из различных почвенных образцов, отобранных с полей коммун Ан Хань, Ан Нинь, Кунь Ми, района Кунь Фу, провинции Тхай Бинь, выделено 48 штаммов бактерий, из которых были отобраны 5 штаммов (АТ 16 VK 4.7 VK 4.8 VK 4.12 VK 4.13), обладающих высокой ингибирующей активностью по отношению к Rhizoctonia solani, которая составила 11,11 62,22. Показано, что эти штаммы обладают способностью синтезировать такой фитогормон, как индолилуксусную кислоту (ИУК) (с 9,54 мг/мл до 31,06 мг/мл), растворять труднорастворимые фосфатные соединения и секрецировать сидерофор. Из 5 выделенных штаммов только у штамма АТ 16 данные способности выражены более сильно. Полученные результаты свидетельствуют о том, что изученные штаммы обладают хорошим потенциалом для использования их в качестве биологических агентов, контролирующих развитие R. solani на растениях перца.Peppers or chilli (Capsicum annuum L.) is widely grown in Vietnam because they bring considerable income to farmers. On pepper fields, diseases such as rhizoctonia, Rhizoctoniasolani caused by fungus, bacterial wilt by Ralstoniasolanacearum are often observed. Rhizoctoniasolani causes a wide range of significant diseases such as collar rot, root rot, damping offin horticultural and field crops.The diseases caused by rhizoctonia are one of the most harmful diseases on peppers, can be observed in any phase of plant development. Rhizobacteria that stimulate plant growth can be used to improve plant growth and suppress plant diseases. The purpose of this work is to isolate bacteria from the pepper rhizosphere that inhibit Rhizoctoniasolani and evaluate in vitro their phosphate solubilizing activity and production of siderophore. Of the different soil samples taken from the pepper fields of An Thanh, An Ninh, Quynh My, QuynhPhudistrict, ThaiBinh province, 48 bacterial strains were isolated. Of these, 5 strains (AT16, VK 4.7, VK 4.8, VK 4.12, VK 4.13) expressed as higher inhibitory Rhizoctoniasolani activity were selected. Their inhibitory activity is from 11.11 to 62.22. These strains have the ability to synthesize phytohormone IAA (from 9.54 g / ml to 31.06g / ml), solubilize the phosphate compounds and productsiderophore. Strain AT16 more effective expresses these abilities in comparison with 4 other strains. These results suggest that the selected strains have excellent potential for use as biologically controlled agents of R. solani on pepper plants (Capsicum annuum L.).


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