Effects of Lead on the Morphology and Structure of the Nucleolus in the Root Tip Meristematic Cells of Allium cepa L.

Objective: The current study is designed to evaluate the cyto-depressive effects of Ganoderma lucidum (GL) and Pleurotus ostreatus (PO) and their binary amalgams (GL+PO) on meristematic cells of Allium cepa L. Methods: Experiments were assessed by utilizing A. cepa bioassay. Results: Onion knobs were exposed to 500 μg/mL, 1000 μg/mL, and 2000 μg/mL groupings of the concentrates for naturally visible and minute examination. The concentrates essentially repressed the root development of A. cepa L. contrasted with the control in a period of treatment and concentration-dependent manner (p<0.05). In addition, photomicrographs revealed chromosomal aberrations extending from c-mitosis, stickiness, and bridges in the root tip meristematic cells of A. cepa. All mushroom extracts demonstrated remarkable cyto-depressive impacts on meristematic cells of A. cepa L. with some changeability. Conclusion: In view of the results come out, GL and PO could be considered as potential sources of anticancer mixes. Notwithstanding, additionally thinks about are essential for disengagement and portrayal of the bioactive mixes and more examination on their pharmacological properties is required. It is first report in Bangladesh where we studied cyto-depressive role of edible mushrooms, Ganoderma lucidum (GL) and Pleurotus ostreatus (PO).

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Oxidative stress and cytogenetic effects in root tip cells of Allium cepa L. induced by alcoholic extracts of Leptinotarsa decemlineata (Say)

Caryologia ◽

10.1080/00087114.2018.1486117 ◽

2018 ◽

Vol 71 (4) ◽

pp. 405-413

Author(s):

Nicoleta Anca Şuţan ◽

Georgiana Uţă ◽

Daniela Bărbuceanu

Keyword(s):

Oxidative Stress ◽

Allium Cepa ◽

Leptinotarsa Decemlineata ◽

Root Tip ◽

Cytogenetic Effects ◽

Allium Cepa L ◽

Leptinotarsa Decemlineata Say ◽

Tip Cells ◽

Root Tip Cells

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Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots

Biotemas ◽

10.5007/2175-7925.2015v28n1p45 ◽

2014 ◽

Vol 28 (1) ◽

pp. 45 ◽

Cited By ~ 2

Author(s):

Layana Menezes da Silva ◽

Ana Paula Peron ◽

Francisco Ronielson da Silva Carvalho ◽

Livia Martins ◽

Iana Felício Bantim Calou ◽

...

Keyword(s):

Allium Cepa ◽

Antiproliferative Effect ◽

Hydroalcoholic Extract ◽

Meristematic Cells ◽

Allium Cepa L

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Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2002.025 ◽

2014 ◽

Vol 71 (3) ◽

pp. 211-221 ◽

Cited By ~ 1

Author(s):

Agnieszka Majewska ◽

Mirosława Furmanowa ◽

Kazimierz Głowniak ◽

Joanna Guzewska ◽

Alicja Zobel ◽

...

Keyword(s):

Allium Cepa ◽

Chromatin Condensation ◽

Preprophase Band ◽

Taxus Baccata ◽

Cell Nuclei ◽

Root Tips ◽

Meristematic Cells ◽

Allium Cepa L ◽

Interphase Cells ◽

Average Size

We investigated the influence of extract from <em>Taxus baccata</em> var. Elegantissima (TbE) shoots in 1:8 dilution, containing paclitaxel in concentration of 81,6 µg/g fresh mass on ultrastructure and tubulin cytoskeleton of meristematic cells of <em>Allium cepa</em> L. root tips. Incubation time 3, 6, 12 and 24 h was followed with postincubation in water for 12 and 24 h. During shorter incubation (till 12 h) the surface of the cell nuclei decreased and chromatin became condensed (in comparison to control) but after 24 h the average surface increased and chromatin condensation decreased. In the course of incubation the average size of plastids and vacuoles increased. Moreover, after treatment mitochondria and plastids showed degradation of ultrastructure, which was reversed after 12 h of postincubation. Immunocytochemical assays demonstrated that in the course of incubation in the ThE extract, the tubulin cytoskeleton became partially disorganised. In most interphase cells, cortical microtubules (MTs) lost their oval transverse orientation. The preprophase band (PPB) position in the cell was often asymmetrical. The MTs array of the karyokinetic spindle and phragmoplast was also disturbed. These alterations were completely reversed during postincubation.

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