scholarly journals CYTO-DEPRESSIVE POTENTIAL OF GANODERMA LUCIDUM, PLEUROTUS OSTREATUS AND THEIR BINARY COMBINATIONS AGAINST MERISTEMATIC CELLS OF ALLIUM CEPA L.

2019 ◽  
pp. 320-324
Author(s):  
MOYEN UDDIN PK
Keyword(s):  
Allium Cepa ◽  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Root Tip ◽  
Pharmacological Properties ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Meristematic Cells ◽  
Allium Cepa L

Objective: The current study is designed to evaluate the cyto-depressive effects of Ganoderma lucidum (GL) and Pleurotus ostreatus (PO) and their binary amalgams (GL+PO) on meristematic cells of Allium cepa L. Methods: Experiments were assessed by utilizing A. cepa bioassay. Results: Onion knobs were exposed to 500 μg/mL, 1000 μg/mL, and 2000 μg/mL groupings of the concentrates for naturally visible and minute examination. The concentrates essentially repressed the root development of A. cepa L. contrasted with the control in a period of treatment and concentration-dependent manner (p<0.05). In addition, photomicrographs revealed chromosomal aberrations extending from c-mitosis, stickiness, and bridges in the root tip meristematic cells of A. cepa. All mushroom extracts demonstrated remarkable cyto-depressive impacts on meristematic cells of A. cepa L. with some changeability. Conclusion: In view of the results come out, GL and PO could be considered as potential sources of anticancer mixes. Notwithstanding, additionally thinks about are essential for disengagement and portrayal of the bioactive mixes and more examination on their pharmacological properties is required. It is first report in Bangladesh where we studied cyto-depressive role of edible mushrooms, Ganoderma lucidum (GL) and Pleurotus ostreatus (PO).

scholarly journals Effects of Lead on the Morphology and Structure of the Nucleolus in the Root Tip Meristematic Cells of Allium cepa L.

2014 ◽  
Vol 15 (8) ◽  
pp. 13406-13423 ◽  
Author(s):  
Ze Jiang ◽  
Huaning Zhang ◽  
Rong Qin ◽  
Jinhua Zou ◽  
Junran Wang ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Root Tip ◽  
Meristematic Cells ◽  
Allium Cepa L ◽  
Morphology And Structure

scholarly journals Effects of L-lysine on cytogenetical and physiological parameters in Allium cepa L. under salt stress

2019 ◽  
Vol 48 (3) ◽  
pp. 625-632
Author(s):  
Dilek Çavuşoğlu
Keyword(s):  
Seed Germination ◽  
Allium Cepa ◽  
Seedling Growth ◽  
Chromosomal Abnormalities ◽  
Inhibitory Effect ◽  
Nacl Stress ◽  
Physiological Parameters ◽  
Root Tip ◽  
Allium Cepa L

The role of L-lysine (Lys) on some cytogenetic and physiological parameters in Allium cepa L. seeds exposed to salt was evaluated. NaCl stress on the other hand showed a significantly inhibitory effect on the seedling growth and seed germination of Allium cepa. Besides, it significantly reduced the mitotic index in the root tip meristems of seeds and increased micronuclei which are the most effective and simplest indicator of cytological damage and chromosomal abnormalities. However, the effects of salinity on chromosomal aberrations, seedling growth, seed germination and mitotic activity have decreased significantly with Lys application.

The P-element-induced silencing effect of KP transposons is dose dependent in Drosophila melanogaster

Genome ◽  
2011 ◽  
Vol 54 (9) ◽  
pp. 752-762 ◽  
Author(s):  
Alireza Sameny ◽  
John Locke
Keyword(s):  
Drosophila Melanogaster ◽  
Critical Role ◽  
Mutagenic Effect ◽  
P Element ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
P Elements ◽  
Single Well ◽  
Dose Dependent

Transposable elements are found in the genomes of all eukaryotes and play a critical role in altering gene expression and genome organization. In Drosophila melanogaster, transposable P elements are responsible for the phenomenon of hybrid dysgenesis. KP elements, a deletion-derivative of the complete P element, can suppress this mutagenic effect. KP elements can also silence the expression of certain other P-element-mediated transgenes in a process called P-element-dependent silencing (PDS), which is thought to involve the recruitment of heterochromatin proteins. To explore the mechanism of this silencing, we have mobilized KP elements to create a series of strains that contain single, well-defined KP insertions that show PDS. To understand the quantitative role of KP elements in PDS, these single inserts were combined in a series of crosses to obtain genotypes with zero, one, or two KP elements, from which we could examine the effect of KP gene dose. The extent of PDS in these genotypes was shown to be dose dependent in a logarithmic rather than linear fashion. A logarithmic dose dependency is consistent with the KP products interacting with heterochromatic proteins in a concentration-dependent manner such that two molecules are needed to induce gene silencing.

Protein kinase C potentiates cAMP-stimulated mouse duodenal mucosal bicarbonate secretion in vitro

2004 ◽  
Vol 286 (5) ◽  
pp. G814-G821 ◽  
Author(s):  
Bi-Guang Tuo ◽  
Jimmy Y. C. Chow ◽  
Kim E. Barrett ◽  
Jon I. Isenberg
Keyword(s):  
Short Circuit ◽  
Short Circuit Current ◽  
Duodenal Mucosa ◽  
Bicarbonate Secretion ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Ussing Chambers ◽  
Duodenal Bicarbonate Secretion

PKC has been shown to regulate epithelial Cl- secretion in a variety of models. However, the role of PKC in duodenal mucosal bicarbonate secretion is less clear. We aimed to investigate the role of PKC in regulation of duodenal mucosal bicarbonate secretion. Bicarbonate secretion by murine duodenal mucosa was examined in vitro in Ussing chambers using a pH-stat technique. PKC isoform expression and activity were assessed by Western blotting and in vitro kinase assays, respectively. PMA (an activator of PKC) alone had no effect on duodenal bicarbonate secretion or short-circuit current ( Isc). When PMA and dibutyryl-cAMP (db-cAMP) were added simultaneously, PMA failed to alter db-cAMP-stimulated duodenal bicarbonate secretion or Isc ( P > 0.05). However, a 1-h preincubation with PMA potentiated db-cAMP-stimulated duodenal bicarbonate secretion and Isc in a concentration-dependent manner (from 10-8 to 10-5M) ( P < 0.05). PMA preincubation had no effects on carbachol- or heat-stable toxin-stimulated bicarbonate secretion. Western blot analysis revealed that PKCα, -γ, -ϵ, -θ, -μ, and -ι/λ were expressed in murine duodenal mucosa. Ro 31–8220 (an inhibitor active against PKCϵ, -α, -β, and -γ), but not Gö 6983 (an inhibitor active against PKCα, -γ, -β, and -δ), reversed the potentiating effect of PMA on db-cAMP-stimulated bicarbonate secretion. PMA also time- and concentration-dependently increased the activity of PKCϵ, an effect that was prevented by Ro 31–8220 but not Gö 6983. These results demonstrate that activation of PKC potentiates cAMP-stimulated duodenal bicarbonate secretion, whereas it does not modify basal secretion. The effect of PKC on cAMP-stimulated bicarbonate secretion is mediated by the PKCϵ isoform.

scholarly journals Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots

Biotemas ◽  
2014 ◽  
Vol 28 (1) ◽  
pp. 45 ◽  
Author(s):  
Layana Menezes da Silva ◽  
Ana Paula Peron ◽  
Francisco Ronielson da Silva Carvalho ◽  
Livia Martins ◽  
Iana Felício Bantim Calou ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Antiproliferative Effect ◽  
Hydroalcoholic Extract ◽  
Meristematic Cells ◽  
Allium Cepa L

scholarly journals Sec1p directly stimulates SNARE-mediated membrane fusion in vitro

2004 ◽  
Vol 167 (1) ◽  
pp. 75-85 ◽  
Author(s):  
Brenton L. Scott ◽  
Jeffrey S. Van Komen ◽  
Hassan Irshad ◽  
Song Liu ◽  
Kirilee A. Wilson ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Membrane Fusion ◽  
Membrane Trafficking ◽  
Snare Complex ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Bud Neck ◽  
Precise Role

Sec1 proteins are critical players in membrane trafficking, yet their precise role remains unknown. We have examined the role of Sec1p in the regulation of post-Golgi secretion in Saccharomyces cerevisiae. Indirect immunofluorescence shows that endogenous Sec1p is found primarily at the bud neck in newly budded cells and in patches broadly distributed within the plasma membrane in unbudded cells. Recombinant Sec1p binds strongly to the t-SNARE complex (Sso1p/Sec9c) as well as to the fully assembled ternary SNARE complex (Sso1p/Sec9c;Snc2p), but also binds weakly to free Sso1p. We used recombinant Sec1p to test Sec1p function using a well-characterized SNARE-mediated membrane fusion assay. The addition of Sec1p to a traditional in vitro fusion assay moderately stimulates fusion; however, when Sec1p is allowed to bind to SNAREs before reconstitution, significantly more Sec1p binding is detected and fusion is stimulated in a concentration-dependent manner. These data strongly argue that Sec1p directly stimulates SNARE-mediated membrane fusion.

scholarly journals Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots

2014 ◽  
Vol 71 (3) ◽  
pp. 211-221 ◽  
Author(s):  
Agnieszka Majewska ◽  
Mirosława Furmanowa ◽  
Kazimierz Głowniak ◽  
Joanna Guzewska ◽  
Alicja Zobel ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Chromatin Condensation ◽  
Preprophase Band ◽  
Taxus Baccata ◽  
Cell Nuclei ◽  
Root Tips ◽  
Meristematic Cells ◽  
Allium Cepa L ◽  
Interphase Cells ◽  
Average Size

We investigated the influence of extract from <em>Taxus baccata</em> var. Elegantissima (TbE) shoots in 1:8 dilution, containing paclitaxel in concentration of 81,6 µg/g fresh mass on ultrastructure and tubulin cytoskeleton of meristematic cells of <em>Allium cepa</em> L. root tips. Incubation time 3, 6, 12 and 24 h was followed with postincubation in water for 12 and 24 h. During shorter incubation (till 12 h) the surface of the cell nuclei decreased and chromatin became condensed (in comparison to control) but after 24 h the average surface increased and chromatin condensation decreased. In the course of incubation the average size of plastids and vacuoles increased. Moreover, after treatment mitochondria and plastids showed degradation of ultrastructure, which was reversed after 12 h of postincubation. Immunocytochemical assays demonstrated that in the course of incubation in the ThE extract, the tubulin cytoskeleton became partially disorganised. In most interphase cells, cortical microtubules (MTs) lost their oval transverse orientation. The preprophase band (PPB) position in the cell was often asymmetrical. The MTs array of the karyokinetic spindle and phragmoplast was also disturbed. These alterations were completely reversed during postincubation.

Sign in / Sign up

Export Citation Format

Share Document