Oleic Acid Uptake Reveals the Rescued Enterocyte Phenotype of Colon Cancer Caco-2 by HT29-MTX Cells in Co-Culture Mode

AbstractPrimary human myotubes represent an alternative system to intact skeletal muscle for the study of human diseases related to changes in muscle energy metabolism. This work aimed to study if fatty acid and glucose metabolism in human myotubes in vitro were related to muscle of origin, donor gender, age, or body mass index (BMI). Myotubes from a total of 82 donors were established from three different skeletal muscles, i.e., musculus vastus lateralis, musculus obliquus internus abdominis, and musculi interspinales, and cellular energy metabolism was evaluated. Multiple linear regression analyses showed that donor age had a significant effect on glucose and oleic acid oxidation after correcting for gender, BMI, and muscle of origin. Donor BMI was the only significant contributor to cellular oleic acid uptake, whereas cellular glucose uptake did not rely on any of the variables examined. Despite the effect of age on substrate oxidation, cellular mRNA expression of pyruvate dehydrogenase kinase 4 (PDK4) and peroxisome proliferator–activated receptor gamma coactivator 1 alpha (PPARGC1A) did not correlate with donor age. In conclusion, donor age significantly impacts substrate oxidation in cultured human myotubes, whereas donor BMI affects cellular oleic acid uptake.

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Synthesis and preliminary evaluation of an 18 F-labeled oleic acid analog for PET imaging of fatty acid uptake and metabolism

Nuclear Medicine and Biology ◽

10.1016/j.nucmedbio.2015.08.005 ◽

2016 ◽

Vol 43 (1) ◽

pp. 108-115 ◽

Cited By ~ 4

Author(s):

Zhengxin Cai ◽

Neale Scott Mason ◽

Carolyn J. Anderson ◽

Wilson Barry Edwards

Keyword(s):

Oleic Acid ◽

Fatty Acid ◽

Pet Imaging ◽

Fatty Acid Uptake ◽

Preliminary Evaluation ◽

Acid Uptake ◽

Acid Analog ◽

Uptake And Metabolism

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Oleic acid uptake into rat and rabbit jejunal brush border membrane

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(95)00035-2 ◽

1995 ◽

Vol 1236 (1) ◽

pp. 51-64 ◽

Cited By ~ 20

Author(s):

C Schoeller ◽

M Keelan ◽

G Mulvey ◽

W Stremmel ◽

A.B.R Thomson

Keyword(s):

Oleic Acid ◽

Brush Border ◽

Brush Border Membrane ◽

Acid Uptake

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Relationships Between Oleic Acid Uptake and Lipid Metabolism inMycobacterium smegmatis1

American Review of Respiratory Disease ◽

10.1164/arrd.1972.106.3.450 ◽

1972 ◽

Vol 106 (3) ◽

pp. 450-457 ◽

Cited By ~ 11

Author(s):

Molly P. Weir ◽

W. H. R. Langridge ◽

R. W. Walker

Keyword(s):

Oleic Acid ◽

Lipid Metabolism ◽

Acid Uptake

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Effect of calcium and mineral waters on oleic-acid uptake by isolated hamster enterocytes

Reproduction Nutrition Development ◽

10.1051/rnd:19940505 ◽

1994 ◽

Vol 34 (5) ◽

pp. 439-448 ◽

Cited By ~ 2

Author(s):

J. Goré ◽

C. Hoinard ◽

JM Antoine ◽

C. Couet

Keyword(s):

Oleic Acid ◽

Mineral Waters ◽

Acid Uptake

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Interaction of non-esterified fatty acid and insulin in control of triacylglycerol secretion by Hep G2 cells

Biochemical Journal ◽

10.1042/bj2800099 ◽

1991 ◽

Vol 280 (1) ◽

pp. 99-104 ◽

Cited By ~ 48

Author(s):

C D Byrne ◽

N P J Brindle ◽

T W M Wang ◽

C N Hales

Keyword(s):

Oleic Acid ◽

Fatty Acid ◽

Acid Uptake ◽

Hep G2 ◽

Hep G2 Cells ◽

Initial Concentration ◽

Triacylglycerol Metabolism ◽

Triacylglycerol Synthesis ◽

Rat Liver Cells ◽

G2 Cells

The role of insulin in the regulation of plasma triacylglycerol is poorly understood. Conflicting actions of insulin on rat liver cells have been reported, insulin inhibiting triacylglycerol secretion in short incubations (less than 24 h) and stimulating triacylglycerol secretion in longer incubations (48-72 h). The present study was undertaken to examine regulation of triacylglycerol secretion by insulin and investigate the interaction between insulin and non-esterified fatty acid over 72 h in human hepatoblastoma (Hep G2) cells. Insulin inhibited triacylglycerol secretion throughout the 72 h period. The inhibition increased from 66% in the first 24 h to 88% in the final 24 h. Increasing the initial concentration of oleic acid from 200 microM to 1000 microM resulted in a 358% increase in triacylglycerol secretion and a 712% increase in accumulation over 24 h. Oleic acid uptake by the cells was rapid, with only 2.4% of the initial concentration (500 microM) remaining after 24 h. Supplementation of the medium with oleic acid to maintain the concentration between 750 microM and 1000 microM throughout a 5 h period resulted in a 350% increase in triacylglycerol secretion. Supplementation also decreased the insulin-induced inhibition of triacylglycerol secretion (18.2 to 7.8%; P less than 0.001). These results demonstrate that there is not a biphasic action of insulin on triacylglycerol secretion by Hep G2 cells. Experiments of this nature have not previously taken into account the rapid uptake of non-esterified fatty acid by hepatocytes and have consequently underestimated the effect of a sustained concentration on triacylglycerol metabolism. Oleic acid is therefore an even more potent stimulus to triacylglycerol synthesis and secretion than has previously been recognized. In addition, in the presence of a sustained increase in oleic acid concentration, there is a decrease in the action of insulin to inhibit triacylglycerol secretion.

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