scholarly journals Effects of SCCO2, Gamma Irradiation, and Sodium Dodecyl Sulfate Treatments on the Initial Properties of Tendon Allografts

2020 ◽  
Vol 21 (5) ◽  
pp. 1565 ◽  
Author(s):  
Yikan Sun ◽  
Vedran Lovric ◽  
Tian Wang ◽  
Rema A. Oliver ◽  
William R. Walsh
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Gamma Irradiation ◽  
Ftir Spectroscopy ◽  
Mechanical Testing ◽  
Treatment Time ◽  
Sodium Dodecyl ◽  
Chemical Additives ◽  
Starting Point ◽  
Dodecyl Sulfate ◽  
Gamma Irradiated

Sterile and decellularized allograft tendons are viable biomaterials used in reconstructive surgeries for dense connective tissue injuries. Established allograft processing techniques including gamma irradiation and sodium dodecyl sulfate (SDS) can affect tissue integrity. Supercritical carbon dioxide (SCCO2) represents a novel alternative that has the potential to decellularize and sterilize tendons with minimized exposure to denaturants, shortened treatment time, lack of toxic residues, and superior tissue penetration, and thus efficacy. This study attempted to develop a single-step hybrid decellularization and sterilization protocol for tendons that involved SCCO2 treatment with various chemical additives. The processed tendons were evaluated with mechanical testing, histology, scanning electron microscopy (SEM), and Fourier-transform infrared (FTIR) spectroscopy. Uniaxial mechanical testing showed that tendons treated with SCCO2 and additive NovaKillTM Gen2 and 0.1% SDS had significantly higher (p < 0.05) ultimate tensile stress (UTS) and Young’s modulus compared to gamma-irradiated and standard-SDS-treated tendons. This was corroborated by the ultrastructural intactness of SCCO2-treated tendons as examined by SEM and FTIR spectroscopy, which was not preserved in gamma-irradiated and standard SDS-treated tendons. However, complete decellularization was not achieved by the experimented SCCO2-SDS protocols used in this study. The present study therefore serves as a concrete starting point for development of an SCCO2-based combined sterilization and decellularization protocol for allograft tendons, where additive choice is to be optimized.

Efficacy of Sanitizers in Reducing Salmonella on Pecan Nutmeats during Cracking and Shelling

2013 ◽  
Vol 76 (5) ◽  
pp. 770-778 ◽  
Author(s):  
LARRY R. BEUCHAT ◽  
DAVID A. MANN ◽  
WALID Q. ALALI
Keyword(s):  
Lactic Acid ◽  
Sodium Dodecyl Sulfate ◽  
Atmospheric Pressure ◽  
Levulinic Acid ◽  
Treatment Time ◽  
Sodium Dodecyl ◽  
Department Of Agriculture ◽  
Dodecyl Sulfate

Studies were done to evaluate the efficacy of chlorine (200 to 1,000 μg/ml), lactic acid (0.5 to 2%), levulinic acid (0.5 to 2%), sodium dodecyl sulfate (SDS, 0.05%), lactic acid plus SDS, levulinic acid plus SDS, and a mixed peroxyacid sanitizer (Tsunami 200, 40 and 80 μg/ml) in killing Salmonella on or in immersion- and on surface-inoculated pecan nutmeats (U.S. Department of Agriculture medium pieces and mammoth halves). The addition of SDS to treatment solutions containing lactic acid or levulinic acid resulted in generally higher reductions of Salmonella, but differences in these reductions were not always significant. Lactic and levulinic acids (2%) containing SDS (0.05%) were equivalent in killing Salmonella on immersion-inoculated nutmeats. Tsunami 200 (40 μg/ml) was less lethal or equivalent to 1 or 2% lactic and levulinic acids, with or without 0.05% SDS. Reductions did not exceed 1.1 log CFU/g of immersion-inoculated pieces and halves, regardless of sanitizer concentration or treatment time (up to 20 min). Reductions on surface-inoculated pieces and halves were 0.7 to 2.6 log CFU/g and 1.2 to 3.0 log CFU/g, respectively. Treatment with 2% lactic acid plus SDS (0.05%) and Tsunami (80 μg/ml) was most effective in killing Salmonella on surface-inoculated pieces; treatment of halves with chlorine (1,000 μg/ml) or lactic acid (1 or 2%), with or without SDS, was most efficacious. Exposure of immersion-inoculated pecan pieces to chlorine (200 μg/ml), lactic acid (2%) and levulinic acid (2%) with or without SDS, and Tsunami (80 μg/ml) during intermittent vacuum (18 ± 2 mbar) and ambient atmospheric pressure treatments for up to 20 min reduced Salmonella by only 0.1 to 1.0 log CFU/g. These studies emphasize the importance of preventing contamination of pecan nutmeats with Salmonella. Once nuts are contaminated, the lethality of sanitizers tested in this study is minimal.

scholarly journals Sodium dodecyl sulfate as a viral inactivator and future perspectives in the control of small ruminant lentiviruses

2019 ◽  
Vol 86 ◽  
Author(s):  
Ana Lídia Madeira de Sousa ◽  
Raymundo Rizaldo Pinheiro ◽  
Juscilânia Furtado Araújo ◽  
Dalva Alana Aragão de Azevedo ◽  
Renato Mesquita Peixoto ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Small Ruminant ◽  
Sodium Dodecyl ◽  
Economic Losses ◽  
Chemical Additives ◽  
Route Of Transmission ◽  
Dodecyl Sulfate ◽  
Ovine Lentivirus ◽  
Small Ruminant Lentiviruses ◽  
Microbiological Agents

ABSTRACT Infections by small ruminant lentiviruses (SRLVs) affect goats and sheep causing chronic multisystemic diseases that generate great economic losses. The caprine lentivirus (CLV) and the ovine lentivirus (OLV) present tropism for cells of the monocyte/macrophage lineage, which are directly associated with the main route of transmission through the ingestion of milk and colostrum from infected animals. In this manner, controlling this route is of paramount importance. Currently, researches have investigated the use of chemical additives in milk that can preserve colostrum or milk and inactivate microbiological agents. Among the compounds, sodium dodecyl sulfate (SDS) has been shown to be satisfactory in the chemical inactivation of HIV and CLV in milk, and also as a biocide in goat colostrum.

Solubility and adsorption behaviors of chlorophenols in the presence of surfactant

1997 ◽  
Vol 35 (7) ◽  
pp. 123-130 ◽  
Author(s):  
J. C. Liu ◽  
P. S. Chang
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Critical Micelle Concentration ◽  
Sodium Dodecyl ◽  
Adsorption Behaviors ◽  
Brij 35 ◽  
Important Index ◽  
Dodecyl Sulfate ◽  
Triton X

The solubility of chlorophenols as affected by surfactant was investigated. Three kinds of surfactant, sodium dodecyl sulfate, Triton X-100, and Brij 35, were utilized. The solubilization of chlorophenols by surfactant follows the order of 2,4,6-trichlorophenol &gt; 2,4-dichlorophenol &gt; 2,6-dichlorophenol &gt; 2-chlorophenol; and the critical micelle concentration is an important index. The adsorption reactions of 2,4-dichlorophenol and 2,4,6- trichlorophenol onto hydrous montmorillonite in the presence of surfactant were examined. The presence of surfactant decreased the adsorption of chlorophenols significantly. The roles of hydrophobicity of chlorophenols in solubilization and adsorption behaviors are discussed.

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