scholarly journals Human Wharton’s Jelly Mesenchymal Stem Cell-Mediated Sciatic Nerve Recovery Is Associated with the Upregulation of Regulatory T Cells

2020 ◽  
Vol 21 (17) ◽  
pp. 6310
Author(s):  
Aline Yen Ling Wang ◽  
Charles Yuen Yung Loh ◽  
Hsin-Hsin Shen ◽  
Sing-Ying Hsieh ◽  
Ing-Kae Wang ◽  
...  
Keyword(s):  
T Cells ◽  
Sciatic Nerve ◽  
Regulatory T Cells ◽  
Inflammatory Cytokines ◽  
Peripheral Nerve Regeneration ◽  
Wharton’S Jelly ◽  
Treg Depletion ◽  
Wharton's Jelly ◽  
Nerve Recovery ◽  
Anti Inflammatory

The acceleration of peripheral nerve regeneration is crucial for functional nerve recovery. Our previous study demonstrated that human Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSC) promote sciatic nerve recovery and regeneration via the direct upregulation and release of neurotrophic factors. However, the immunomodulatory role of hWJ-MSC in sciatic nerve recovery remains unclear. The effects of hWJ-MSC on innate immunity, represented by macrophages, natural killer cells, and dendritic cells, as well as on adaptive immunity, represented by CD4+ T, CD8+ T, B, and regulatory T cells (Tregs), were examined using flow cytometry. Interestingly, a significantly increased level of Tregs was detected in blood, lymph nodes (LNs), and nerve-infiltrating cells on POD7, 15, 21, and 35. Anti-inflammatory cytokines, such as IL-4 and IL-10, were significantly upregulated in the LNs and nerves of hWJ-MSC-treated mice. Treg depletion neutralized the improved effects of hWJ-MSC on sciatic nerve recovery. In contrast, Treg administration promoted the functional recovery of five-toe spread and gait stance. hWJ-MSC also expressed high levels of the anti-inflammatory cytokines TGF-β and IL-35. This study indicated that hWJ-MSC induce Treg development to modulate the balance between pro- and anti-inflammation at the injured sciatic nerve by secreting higher levels of anti-inflammatory cytokines.

scholarly journals From 'perfect mix' to 'potion magique' — regulatory T cells and anti-inflammatory cytokines as adjuvant targets

2008 ◽  
Vol 6 (1) ◽  
pp. 88-88 ◽  
Author(s):  
Jagadeesh Bayry ◽  
Darren R. Flower ◽  
David F. Tough ◽  
Srini V. Kaveri
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Inflammatory Cytokines ◽  
Anti Inflammatory

The influence of sex hormones on cytokines in multiple sclerosis and experimental autoimmune encephalomyelitis: a review

2005 ◽  
Vol 11 (3) ◽  
pp. 349-359 ◽  
Author(s):  
Hans HLP van den Broek ◽  
Jan GMC Damoiseaux ◽  
Marc H De Baets ◽  
Raymond MM Hupperts
Keyword(s):  
Multiple Sclerosis ◽  
Immune Response ◽  
T Cells ◽  
Regulatory T Cells ◽  
Sex Hormones ◽  
Inflammatory Cytokines ◽  
Cytokine Production ◽  
Anti Inflammatory ◽  
Experimental Autoimmune

The female predominance of multiple sclerosis (MS) has suggested that hormonal differences between the sexes must confer some protective effect on males or enhance the susceptibility of females to this disease. There has been evidence that gonadal hormones can modulate the immune response regulated by antigen presenting cells and T cells. These cells control the immune response by the production of interacting pro- and anti-inflammatory cytokines. The first include the acute phase pro-inflammatory cytokines of the innate immune response as well as the T-helper 1 (Th1) cytokines, while the later contain the Th2 cytokines as well as the suppressor cytokines. There is some evidence that MS and experimental autoimmune encephalitis (EAE) are Th1 cell-mediated diseases. For this reason many studies have been done to influence the pro-inflammatory cytokine production of these Th1 cells in favour of an anti-inflammatory immune response as mediated by Th2 cells. However the role of the regulatory T cells in this context is not clearly understood. Here we review the studies concerning the role of sex hormones on the cytokine production in relation to the disease course of MS and EAE and in particular in the light of the recent revival of the regulatory T cells and their suppressive cytokines.

scholarly journals Topical Application of Human Wharton’s Jelly Mesenchymal Stem Cells Accelerates Mouse Sciatic Nerve Recovery and is Associated with Upregulated Neurotrophic Factor Expression

2019 ◽  
Vol 28 (12) ◽  
pp. 1560-1572 ◽  
Author(s):  
Aline Yen Ling Wang ◽  
Charles Yuen Yung Loh ◽  
Hsin-Hsin Shen ◽  
Sing-Ying Hsieh ◽  
Ing-Kae Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Topical Application ◽  
Neurotrophic Factor ◽  
Negative Control ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Nerve Recovery

Peripheral nerve regeneration following injury is often slow and impaired, which results in weakened and denervated muscle with subsequent atrophy. Human Wharton’s jelly mesenchymal stem cells (hWJ-MSC) have potential regenerative properties which, however, remain unknown in mouse nerve recovery. This study investigated the effect of the topical application of hWJ-MSC onto repairing transected sciatic nerves in a mouse model. Human adipocyte-derived stem cells (hADSC) were used as a positive control. The sciatic nerve of BALB/c mice was transected at a fixed point and repaired under the microscope using 10-0 sutures. hWJ-MSC and hADSC were applied to the site of repair and mice were followed up for 1 year. The hWJ-MSC group had significantly better functional recovery of five-toe spread and gait angles compared with the negative control and hADSC groups. hWJ-MSC improved sciatic nerve regeneration in a dose-dependent fashion. The hWJ-MSC group had a better quality of regenerated nerve with an increased number of myelinated axons throughout. hWJ-MSC appear to be safe in mice after 1 year of follow-up. hWJ-MSC also expressed higher levels of neurotrophic factor-3, brain-derived neurotrophic factor, and glial-derived neurotrophic factor than hADSC. hWJ-MSC may promote better nerve recovery than hADSC because of this upregulation of neurotrophic factors.

Sign in / Sign up

Export Citation Format

Share Document