scholarly journals Fibroblast Growth Factor Type 1 Ameliorates High-Glucose-Induced Oxidative Stress and Neuroinflammation in Retinal Pigment Epithelial Cells and a Streptozotocin-Induced Diabetic Rat Model

2021 ◽  
Vol 22 (13) ◽  
pp. 7233
Author(s):  
Hsin-Wei Huang ◽  
Chung-May Yang ◽  
Chang-Hao Yang
Keyword(s):  
Oxidative Stress ◽  
Growth Factor ◽  
High Glucose ◽  
Diabetic Rat ◽  
Fibroblast Growth ◽  
High Glucose Condition ◽  
Factor Type ◽  
Diabetic Rat Model ◽  
Glucose Condition

Diabetic retinopathy (DR) is a common complication of diabetes that causes severe visual impairment globally. The pathogenesis of DR is related to oxidative stress and chronic inflammation. The fibroblast growth factor type 1 (FGF-1) mitogen plays crucial roles in cell function, development, and metabolism. FGF-1 is involved in blood sugar regulation and exerts beneficial antioxidative and anti-inflammatory effects on various organ systems. This study investigated the antioxidative and anti-inflammatory neuroprotective effects of FGF-1 on high-glucose-induced retinal damage. The results revealed that FGF-1 treatment significantly reversed the harmful effects of oxidative stress and inflammatory mediators in retinal tissue in a streptozotocin-induced diabetic rat model. These protective effects were also observed in the in vitro model of retinal ARPE-19 cells exposed to a high-glucose condition. We demonstrated that FGF-1 attenuated p38 mitogen-activated protein kinase and nuclear factor-κB pathway activation under the high-glucose condition. Our results indicated that FGF-1 could effectively prevent retinal injury in diabetes. The findings of this study could be used to develop novel treatments for DR that aim to reduce the cascade of oxidative stress and inflammatory signals in neuroretinal tissue.

scholarly journals Time-lapse microscopy of oxidative stress demonstrates metabolic sensitivity of retinal pericytes under high glucose condition

2018 ◽  
Vol 11 (9) ◽  
pp. e201700289 ◽  
Author(s):  
Zahra Ghanian ◽  
Shima Mehrvar ◽  
Nasim Jamali ◽  
Nader Sheibani ◽  
Mahsa Ranji
Keyword(s):  
Oxidative Stress ◽  
High Glucose ◽  
Time Lapse ◽  
Time Lapse Microscopy ◽  
Retinal Pericytes ◽  
High Glucose Condition ◽  
Glucose Condition

Insulin-like growth factor type 1 prevents hyperglycemia-induced uncoupling protein 3 down-regulation and oxidative stress

2004 ◽  
Vol 77 (2) ◽  
pp. 285-291 ◽  
Author(s):  
Helena Gustafsson ◽  
Therése Söderdahl ◽  
Gunn Jönsson ◽  
Jan-Ove Bratteng ◽  
Anna Forsby
Keyword(s):  
Oxidative Stress ◽  
Growth Factor ◽  
Uncoupling Protein ◽  
Insulin Like Growth Factor ◽  
Down Regulation ◽  
Uncoupling Protein 3 ◽  
Factor Type ◽  
And Oxidative Stress

scholarly journals Embryonic stem cell-derived extracellular vesicle-mimetic nanovesicles rescue erectile function by enhancing penile neurovascular regeneration in the streptozotocin-induced diabetic mouse

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mi-Hye Kwon ◽  
Kang-Moon Song ◽  
Anita Limanjaya ◽  
Min-Ji Choi ◽  
Kalyan Ghatak ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Embryonic Stem Cell ◽  
Clinical Application ◽  
High Glucose ◽  
Erectile Function ◽  
Embryonic Stem ◽  
Neural Regeneration ◽  
High Glucose Condition ◽  
Glucose Condition

AbstractExtracellular vesicles (EVs) have attracted particular interest in various fields of biology and medicine. However, one of the major hurdles in the clinical application of EV-based therapy is their low production yield. We recently developed cell-derived EV-mimetic nanovesicles (NVs) by extruding cells serially through filters with diminishing pore sizes (10, 5, and 1 μm). Here, we demonstrate in diabetic mice that embryonic stem cell (ESC)-derived EV-mimetic NVs (ESC-NVs) completely restore erectile function (~96% of control values) through enhanced penile angiogenesis and neural regeneration in vivo, whereas ESC partially restores erectile function (~77% of control values). ESC-NVs promoted tube formation in primary cultured mouse cavernous endothelial cells and pericytes under high-glucose condition in vitro; and accelerated microvascular and neurite sprouting from aortic ring and major pelvic ganglion under high-glucose condition ex vivo, respectively. ESC-NVs enhanced the expression of angiogenic and neurotrophic factors (hepatocyte growth factor, angiopoietin-1, nerve growth factor, and neurotrophin-3), and activated cell survival and proliferative factors (Akt and ERK). Therefore, it will be a better strategy to use ESC-NVs than ESCs in patients with erectile dysfunction refractory to pharmacotherapy, although it remains to be solved for future clinical application of ESC.

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