A Systematic Review on Nanoencapsulation Natural Antimicrobials in Foods: In Vitro versus In Situ Evaluation, Mechanisms of Action and Implications on Physical-Chemical Quality

Natural antimicrobials (NA) have stood out in the last decade due to the growing demand for reducing chemical preservatives in food. Once solubility, stability, and changes in sensory attributes could limit their applications in foods, several studies were published suggesting micro-/nanoencapsulation to overcome such challenges. Thus, for our systematic review the Science Direct, Web of Science, Scopus, and Pub Med databases were chosen to recover papers published from 2010 to 2020. After reviewing all titles/abstracts and keywords for the full-text papers, key data were extracted and synthesized. The systematic review proposed to compare the antimicrobial efficacy between nanoencapsulated NA (nNA) and its free form in vitro and in situ studies, since although in vitro studies are often used in studies, they present characteristics and properties that are different from those found in foods; providing a comprehensive understanding of primary mechanisms of action of the nNA in foods; and analyzing the effects on quality parameters of foods. Essential oils and nanoemulsions (10.9–100 nm) have received significant attention and showed higher antimicrobial efficacy without sensory impairments compared to free NA. Regarding nNA mechanisms: (i) nanoencapsulation provides a slow-prolonged release to promote antimicrobial action over time, and (ii) prevents interactions with food constituents that in turn impair antimicrobial action. Besides in vitro antifungal and antibacterial, nNA also demonstrated antioxidant activity—potential to shelf life extension in food. However, of the studies involving nanoencapsulated natural antimicrobials used in this review, little attention was placed on proximate composition, sensory, and rheological evaluation. We encourage further in situ studies once data differ from in vitro assay, suggesting food matrix greatly influences NA mechanisms.

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Inhibition of dentine demineralization by zinc oxide: In vitro and in situ studies

Dental Materials ◽

10.1016/j.dental.2005.02.006 ◽

2005 ◽

Vol 21 (12) ◽

pp. 1170-1177 ◽

Cited By ~ 28

Author(s):

Tsutomu Takatsuka ◽

Keiko Tanaka ◽

Yoichi Iijima

Keyword(s):

Zinc Oxide ◽

In Situ Studies

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Effects of preconditioning and extrusion of linseed on the ruminal biohydrogenation of fatty acids. 2. In vitro and in situ studies

Animal Research ◽

10.1051/animres:2006023 ◽

2006 ◽

Vol 55 (4) ◽

pp. 261-271 ◽

Cited By ~ 4

Author(s):

Fowad Akraim ◽

Marie-Claude Nicot ◽

Pierre Weill ◽

Francis Enjalbert

Keyword(s):

Fatty Acids ◽

In Situ Studies ◽

Ruminal Biohydrogenation

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Materials Sealing Preventing Biofilm Formation in Implant/Abutment Joints: Which Is the Most Effective? A Systematic Review and Meta-Analysis

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-19-00121 ◽

2020 ◽

Vol 46 (2) ◽

pp. 163-171

Author(s):

Cecília Alves de Sousa ◽

Maria Beatriz Bello Taborda ◽

Gustavo Antônio Correa Momesso ◽

Eduardo Passos Rocha ◽

Paulo Henrique dos Santos ◽

...

Keyword(s):

Systematic Review ◽

Biofilm Formation ◽

Meta Analysis ◽

Significance Level ◽

Gutta Percha ◽

Significant Difference ◽

Physical Barriers ◽

External Connection

The purpose of this systematic review was to evaluate the literature available for materials exhibiting the best efficacy in preventing biofilm formation in the interior of implants. We searched PubMed/MEDLINE, Scopus, and Cochrane databases. This review is registered with the PROSPERO database and followed the suitability of the PRISMA protocol. The initial search resulted in 326 articles from the databases. After they were read, 8 articles remained, and the inclusion and exclusion criteria were applied. Six of these 8 articles were classified as in vitro and 2 were classified as in situ. The regions of the implants evaluated ranged from the interface of the pieces to the occlusal upper access of the abutment. The implant connections evaluated the Morse taper, external connection, and internal connection. Meta-analysis of the quantitative data was performed at a significance level of .05. Cotton exhibited poor control of infiltration, even in combination with other materials. Isolated gutta-percha (GP) and polytetrafluoroethylene (PTFE) tape with composite resin (CR) or GP performed better as physical barriers. The best results for chemical barriers were observed by the application of 1% chlorhexidine gluconate (CG) gel, thymol varnish, and the deposition of Ag films onto the surface. The applied meta-analysis did not show a significant difference in comparison between the different types of implant connections (P > .05). The application of CG and thymol varnish antimicrobials was effective in preventing biofilm formation and easy clinical execution; these could be used in combination with CR, GP, and PTFE.

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Effects of short- and long-duration hypothyroidism on hypothalamic–pituitary–adrenal axis function in rats: In vitro and in situ studies

Endocrine ◽

10.1007/s12020-012-9714-z ◽

2012 ◽

Vol 42 (3) ◽

pp. 684-693 ◽

Cited By ~ 9

Author(s):

Elizabeth O. Johnson ◽

Aldo E. Calogero ◽

Mary Konstandi ◽

Themis C. Kamilaris ◽

Sandro La Vignera ◽

...

Keyword(s):

Hypothalamic Pituitary Adrenal Axis ◽

Hypothalamic Pituitary Adrenal ◽

In Situ Studies ◽

Adrenal Axis ◽

Long Duration ◽

Pituitary Adrenal Axis

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O046: Comparison of the in vitro antimicrobial efficacy of amoxicillin/metronidazole or azithromycin - a systematic review

Journal Of Clinical Periodontology ◽

10.1111/jcpe.45_12913 ◽

2018 ◽

Vol 45 ◽

pp. 21-22

Keyword(s):

Systematic Review ◽

Antimicrobial Efficacy

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Studies on Mefenamic Acid Microparticles: Formulation, In Vitro Release, and In Situ Studies in Rats

AAPS PharmSciTech ◽

10.1208/s12249-008-9183-0 ◽

2009 ◽

Vol 10 (1) ◽

pp. 104-112 ◽

Cited By ~ 3

Author(s):

Ferhan Sevgi ◽

Aysu Yurdasiper ◽

Buket Kaynarsoy ◽

Ezgi Turunç ◽

Tamer Güneri ◽

...

Keyword(s):

Mefenamic Acid ◽

In Vitro Release ◽

In Situ Studies ◽

Vitro Release

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Celecoxib Loaded In-Situ Provesicular Powder and Its In-Vitro Cytotoxic Effect for Cancer Therapy: Fabrication, Characterization, Optimization and Pharmacokinetic Evaluation

Pharmaceutics ◽

10.3390/pharmaceutics12121157 ◽

2020 ◽

Vol 12 (12) ◽

pp. 1157

Author(s):

Ali M. Nasr ◽

Sameh S. Elhady ◽

Shady A. Swidan ◽

Noha M. Badawi

Keyword(s):

Fatty Alcohol ◽

Desirability Function ◽

Area Under The Curve ◽

Free Form ◽

Pharmacokinetic Parameters ◽

Anticancer Efficacy ◽

Cox 2

Introduction: Several recent studies have shown that the role of cyclooxygenase 2 (COX-2) in carcinogenesis has become more evident. It affects angiogenesis, apoptosis, and invasion, and plays a key role in the production of carcinogens. It has also been reported that COX-2 inhibitors such as celecoxib (CLX) might play an effective role in preventing cancer formation and progression. Formulation of CLX into nanovesicles is a promising technique to improve its bioavailability and anticancer efficacy. Aim: The aim of this study is to optimize and evaluate the anticancer efficacy of CLX-loaded in-situ provesicular powder composed of surfactants and fatty alcohol-based novel nanovesicles in-vitro and determine its pharmacokinetic parameters in-vivo. Methods: The novel provesicular powders were prepared by the slurry method and optimized by 32 full factorial design using the desirability function. Results: Small mean particle size was achieved by the formed vesicles with value of 351.7 ± 1.76 nm and high entrapment efficacy of CLX in the formed vesicles of 97.53 ± 0.84%. Solid state characterization of the optimized formulation showed that the powder was free flowing, showed no incompatibilities between drug and excipients and showed smooth texture. The cytotoxic study of the optimized formula on HCT-116, HepG-2, A-549, PC-3 and MCF-7 cell lines showed significant increase in activity of CLX compared to its free form. The pharmacokinetic study on albino rabbits after oral administration showed significant increase in the area under the curve (AUC)0–24 h and significantly higher oral relative bioavailability of the optimized formulation compared to Celebrex® 100 mg market product (p < 0.05). Conclusion: All findings of this study suggest the potential improvement of efficacy and bioavailability of CLX when formulated in the form of in-situ provesicular powder composed of surfactants and fatty alcohol-based novel nanovesicles for its repositioned use as an anticancer agent.

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