scholarly journals Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts

2021 ◽  
Vol 22 (22) ◽  
pp. 12375
Author(s):  
Huifeng He ◽  
Dandan Qiao ◽  
Lu Zhang ◽  
Yongxiu Yao ◽  
Hongxia Shao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Virus ◽  
Lithium Chloride ◽  
Antiviral Effect ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Viral Gene ◽  
Dependent Manner ◽  
Marek’S Disease Virus ◽  
Marek’S Disease

To investigate the antiviral effect of lithium chloride (LiCl) on the replication of Marek’s disease virus (MDV) in chicken embryonic fibroblast (CEF) cells, real-time PCR, Western blotting, plaque counting, and indirect immunofluorescence experiments were performed at different time points of LiCl treated CEF cells with virus infection. The results demonstrated that LiCl could affect multiple steps of virus replication and inhibit viral gene expression and protein synthesis in a dose- and time-dependent manner. Moreover, LiCl could directly affect viral infectivity as well. In addition, LiCl significantly affected the gene expression of IFN-β related genes in virus-infected cells. These results indicate that LiCl significantly inhibits MDV replication and proliferation in CEF cells and it has the potential to be used as an antiviral agent against MDV.

scholarly journals Antiviral effect of baicalin on Marek’s disease virus in CEF cells

2020 ◽  
Author(s):  
Fan Yang ◽  
Chun Feng ◽  
Yongxiu Yao ◽  
Aijian Qin ◽  
Hongxia Shao ◽  
...  
Keyword(s):  
Disease Virus ◽  
Antiviral Effect ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Virus Infectivity ◽  
Dependent Manner ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Protein Levels ◽  
Anti Oxidant

Abstract Background: Baicalin, the main metabolic component of S.baicalensis Georgi, has various pharmacological properties including anti-inflammatory, anti-oxidant, anti-apoptotic, anti-bactericidal and anti-viral. The purpose of this study was to investigate the anti-Marek’s disease virus (MDV) activities of baicalin in CEF cells.Results: Here, we showed that baicalin could inhibit viral mRNA, protein levels and overall plaque formation in a time-dependent manner. We also found that baicalin could consistently inhibit MDV replication and directly affect the virus infectivity. Moreover, baicalin treatment has no effect on expression level of antiviral cytokine and inflammatory cytokines in virus infected CEFs.Conclusions: These results demonstrate that baicalin could be a potential drug against MDV infection.

scholarly journals Antiviral effect of baicalin on Marek’s disease virus in CEF cells

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Fan Yang ◽  
Chun Feng ◽  
Yongxiu Yao ◽  
Aijian Qin ◽  
Hongxia Shao ◽  
...  
Keyword(s):  
Disease Virus ◽  
Antiviral Effect ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Virus Infectivity ◽  
Dependent Manner ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Protein Levels ◽  
Anti Oxidant

Abstract Background Baicalin, the main metabolic component of Scutellaria baicalensis Georgi, has various pharmacological properties including anti-inflammatory, anti-oxidant, anti-apoptotic, anti-bactericidal and anti-viral. The purpose of this study was to investigate the anti-Marek’s disease virus (MDV) activities of baicalin in CEF cells. Results Here, we showed that baicalin could inhibit viral mRNA, protein levels and overall plaque formation in a time-dependent manner. We also found that baicalin could consistently inhibit MDV replication and directly affect the virus infectivity. Moreover, baicalin treatment has no effect on expression level of antiviral cytokine and inflammatory cytokines in MDV infected CEFs. Conclusions These results demonstrate that baicalin could be a potential drug against MDV infection.

scholarly journals Antiviral effect of baicalin on Marek’s disease virus in CEF cells

2020 ◽  
Author(s):  
Fan Yang ◽  
Chun Feng ◽  
Yongxiu Yao ◽  
Aijian Qin ◽  
Hongxia Shao ◽  
...  
Keyword(s):  
Disease Virus ◽  
Antiviral Effect ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Virus Infectivity ◽  
Dependent Manner ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Scutellaria Baicalensis Georgi ◽  
Protein Levels

Abstract Background: Baicalin, the main metabolic component of Scutellaria baicalensis Georgi, has various pharmacological properties including anti-inflammatory, anti-oxidant, anti-apoptotic, anti-bactericidal and anti-viral. The purpose of this study was to investigate the anti-Marek’s disease virus (MDV) activities of baicalin in CEF cells. Results: Here, we showed that baicalin could inhibit viral mRNA, protein levels and overall plaque formation in a time-dependent manner. We also found that baicalin could consistently inhibit MDV replication and directly affect the virus infectivity. Moreover, baicalin treatment has no effect on expression level of antiviral cytokine and inflammatory cytokines in MDV infected CEFs. Conclusions: These results demonstrate that baicalin could be a potential drug against MDV infection.

scholarly journals Targeted Editing of the pp38 Gene in Marek’s Disease Virus-Transformed Cell Lines Using CRISPR/Cas9 System

Viruses ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 391 ◽  
Author(s):  
Yaoyao Zhang ◽  
Jun Luo ◽  
Na Tang ◽  
Man Teng ◽  
Vishwanatha R.A.P. Reddy ◽  
...  
Keyword(s):  
Disease Virus ◽  
Cell Lines ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Viral Gene ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Transformed Cell ◽  
Transformed Cell Lines

Marek’s disease virus (MDV), a lymphotropic α-herpesvirus associated with T-cell lymphomas in chickens, is an excellent model for herpesvirus biology and virus-induced oncogenesis. Marek’s disease (MD) is also one of the cancers against which a vaccine was first used. In the lymphomas and lymphoblastoid cell lines (LCLs) derived from them, MDV establishes latent infection with limited gene expression. Although LCLs are valuable for interrogating viral and host gene functions, molecular determinants associated with the maintenance of MDV latency and lytic switch remain largely unknown, mainly due to the lack of tools for in situ manipulation of the genomes in these cell lines. Here we describe the first application of CRISPR/Cas9 editing approach for precise editing of the viral gene phosphoprotein 38 (pp38), a biomarker for latent/lytic switch in MDV-transformed LCLs MDCC-MSB-1 (Marek’s disease cell line MSB-1) and MDCC-HP8. Contradictory to the previous reports suggesting that pp38 is involved in the maintenance of transformation of LCL MSB-1 cells, we show that pp38-deleted cells proliferated at a significant higher rate, suggesting that pp38 is dispensable for the transformed state of these cell lines. Application of CRISPR/Cas9-based gene editing of MDV-transformed cell lines in situ opens up further opportunities towards a better understanding of MDV pathogenesis and virus-host interactions.

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