RNA-seq analysis of viral gene expression in the skin of Marek’s disease virus infected chickens

2019 ◽  
Vol 213 ◽  
pp. 109882
Author(s):  
Lakshmi Sunkaraa ◽  
Syed Mudasir Ahmad ◽  
Mohammad Heidari
Keyword(s):  
Gene Expression ◽  
Disease Virus ◽  
Viral Gene Expression ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Viral Gene ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Rna Seq

scholarly journals Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts

2021 ◽  
Vol 22 (22) ◽  
pp. 12375
Author(s):  
Huifeng He ◽  
Dandan Qiao ◽  
Lu Zhang ◽  
Yongxiu Yao ◽  
Hongxia Shao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Virus ◽  
Lithium Chloride ◽  
Antiviral Effect ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Viral Gene ◽  
Dependent Manner ◽  
Marek’S Disease Virus ◽  
Marek’S Disease

To investigate the antiviral effect of lithium chloride (LiCl) on the replication of Marek’s disease virus (MDV) in chicken embryonic fibroblast (CEF) cells, real-time PCR, Western blotting, plaque counting, and indirect immunofluorescence experiments were performed at different time points of LiCl treated CEF cells with virus infection. The results demonstrated that LiCl could affect multiple steps of virus replication and inhibit viral gene expression and protein synthesis in a dose- and time-dependent manner. Moreover, LiCl could directly affect viral infectivity as well. In addition, LiCl significantly affected the gene expression of IFN-β related genes in virus-infected cells. These results indicate that LiCl significantly inhibits MDV replication and proliferation in CEF cells and it has the potential to be used as an antiviral agent against MDV.

scholarly journals Targeted Editing of the pp38 Gene in Marek’s Disease Virus-Transformed Cell Lines Using CRISPR/Cas9 System

Viruses ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 391 ◽  
Author(s):  
Yaoyao Zhang ◽  
Jun Luo ◽  
Na Tang ◽  
Man Teng ◽  
Vishwanatha R.A.P. Reddy ◽  
...  
Keyword(s):  
Disease Virus ◽  
Cell Lines ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Viral Gene ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Transformed Cell ◽  
Transformed Cell Lines

Marek’s disease virus (MDV), a lymphotropic α-herpesvirus associated with T-cell lymphomas in chickens, is an excellent model for herpesvirus biology and virus-induced oncogenesis. Marek’s disease (MD) is also one of the cancers against which a vaccine was first used. In the lymphomas and lymphoblastoid cell lines (LCLs) derived from them, MDV establishes latent infection with limited gene expression. Although LCLs are valuable for interrogating viral and host gene functions, molecular determinants associated with the maintenance of MDV latency and lytic switch remain largely unknown, mainly due to the lack of tools for in situ manipulation of the genomes in these cell lines. Here we describe the first application of CRISPR/Cas9 editing approach for precise editing of the viral gene phosphoprotein 38 (pp38), a biomarker for latent/lytic switch in MDV-transformed LCLs MDCC-MSB-1 (Marek’s disease cell line MSB-1) and MDCC-HP8. Contradictory to the previous reports suggesting that pp38 is involved in the maintenance of transformation of LCL MSB-1 cells, we show that pp38-deleted cells proliferated at a significant higher rate, suggesting that pp38 is dispensable for the transformed state of these cell lines. Application of CRISPR/Cas9-based gene editing of MDV-transformed cell lines in situ opens up further opportunities towards a better understanding of MDV pathogenesis and virus-host interactions.

scholarly journals Protective Effect of Avian Myelomonocytic Growth Factor in Infection with Marek’s Disease Virus

2002 ◽  
Vol 76 (3) ◽  
pp. 1062-1070 ◽  
Author(s):  
Aouatef Djeraba ◽  
Eugène Musset ◽  
John W. Lowenthal ◽  
David B. Boyle ◽  
Anne-Marie Chaussé ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Growth Factor ◽  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
No Production ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Inos Gene

ABSTRACT Marek’s disease virus (MDV) is a herpesvirus that induces T lymphomas in chickens. The aim of this study was to assess the role of the macrophage activator chicken myelomonocytic growth factor (cMGF) in controlling MDV infection. B13/B13 chickens, which are highly susceptible to MD, were either treated with cMGF delivered via a live fowlpox virus (fp/cMGF) or treated with the parent vector (fp/M3) or were left as untreated controls. Seven days later, when challenged with the very virulent RB-1B strain of MDV, the spleens of chickens treated with fp/cMGF showed increased expression of the inducible nitric oxide synthase (iNOS) gene compared to those of control chickens and fp/M3-treated chickens. Increased iNOS gene expression was also accompanied by greater induction of gamma interferon and macrophage inflammatory protein (K203) gene expression, both possible activators of iNOS. fp/cMGF treatment also increased the number of monocytes and systemic NO production in contrast to fp/M3 treatment. Even though cMGF treatment was unable to prevent death for the chickens, it did prolong their survival time, and viremia and tumor incidence were greatly reduced. In addition, cMGF treatment improved the partial protection induced by vaccination with HVT (herpesvirus isolated from turkeys) against RB-1B, preventing 100% mortality (versus 66% with vaccination alone) and greatly reducing tumor development. Treatment with fp/M3 did not have such effects. These results suggest that cMGF may play multiple roles in protection against MD. First, it may enhance the innate immune response by increasing the number and activity of monocytes and macrophages, resulting in increased NO production. Second, it may enhance the acquired immune response, indicated by its ability to enhance vaccine efficacy.

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