Cold Response of the Mediterranean Fruit Fly (Ceratitis capitata) on a Lab Diet

Cold treatment at 0.0 °C with different exposure durations (0–12 days) was applied to the Mediterranean fruit fly Ceratitis capitata (Wiedemann) fed on a lab diet. The examined developmental stages were early eggs (<6 h), late eggs (>42 h), first instar, second instar and third instar larvae. Pupation, adult emergence and sex ratios of survived flies were investigated to study the C. capitata responses to this low temperature treatment. Our results showed that exposure time at low temperature has a clear effect on pupation and adult emergence. Based on pupation ratios, the first and third instar are the most cold tolerant stages, with LT99 = 7.3 for both of them. Cold tolerance at both stages are very close and no significant differences were detected. There were no significant differences on C. capitata sex ratios among different stages after treatment. This study improves our understanding of C. capitata responses to cold treatment, which may assist in the improvement of the current treatment strategies to control this destructive horticulture pest species.

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Cold Response of the Mediterranean fruit fly (Ceratitis capitata) in the Lab Diet

10.1101/499228 ◽

2018 ◽

Author(s):

Farhan jasim Al-behadili

Keyword(s):

Ceratitis Capitata ◽

Sex Ratios ◽

Cold Treatment ◽

Treatment Strategies ◽

Fruit Fly ◽

Mediterranean Fruit Fly ◽

Pest Species ◽

Cold Response ◽

Cold Tolerant ◽

The Mediterranean

Cold treatment at 0.0 °C with different exposure durations (0-12 days) was applied to the Mediterranean fruit fly Ceratitis capitata (Wiedemann) fed on lab diet. The examined developmental stages were early eggs (<6 hr) (E.E), late eggs (>42 hr)(L.E),first instar (1st ), second instar(2nd ) and third instar larvae (3rd ). Pupation, adult emergence and sex ratios of survived flies were investigated to study the C. capitata responses to this low temperature treatment. Our results showed that based on pupation ratios, the 3rd instar is the most cold tolerant stage with LT99=7.36 days. The second most cold tolerant stage is the 1st instar with LT99=7.33 days. Cold tolerance at both two stages are very close, so they should be paid attention during the cold treatment. There were no significant differences on C. capitata sex ratios among different stages after treatment. This study improves our understanding of C. capitata responses to cold treatment, which may assist in the improvement of the current treatment strategies to control this destructive horticulture pest species

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Mediterranean Fruit Fly Ceratitis capitata (Diptera: Tephritidae) Eggs and Larvae Responses to a Low-Oxygen/High-Nitrogen Atmosphere

Insects ◽

10.3390/insects11110802 ◽

2020 ◽

Vol 11 (11) ◽

pp. 802

Author(s):

Farhan J.M. Al-Behadili ◽

Manjree Agarwal ◽

Wei Xu ◽

Yonglin Ren

Keyword(s):

Ceratitis Capitata ◽

Insect Pests ◽

Sex Ratios ◽

Adult Emergence ◽

Fruit Fly ◽

Nitrogen Atmosphere ◽

Mediterranean Fruit Fly ◽

Low Oxygen ◽

High Nitrogen ◽

Complete Control

The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is one of the most damaging horticultural insect pests. This study used a low-oxygen/high-nitrogen bioassay to control C. capitata. Two low-oxygen treatments were applied (0.5% O2 + 99.5 N2 and 5% O2 + 95% N2) to C. capitata eggs and 1st, 2nd and 3rd instar larvae from 0 to nine days on a carrot diet at 25 °C; 70—75% RH. The pupariation, adult emergence, and sex ratios of survived flies were examined. The results demonstrate that increased mortality of all tested life stages correlated with increased exposure times at both levels of low-oxygen treatments. Complete control of eggs was achieved after eight days and nine days for larvae using 0.5% O2 at 25 °C; 70–75% RH. The 3rd instar was the most tolerant stage, while the egg was the most susceptible stage to the low-oxygen environment. There were no significant differences in sex ratios between emerged adults after low-oxygen and control treatments. The present work demonstrates and confirms the mortalities of C. capitata caused by low-oxygen treatment, which may help develop new postharvest strategies to control this destructive fruit fly pest.

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Insecticidal activity of a Moroccan strain ofStreptomyces phaeochromogenesLD-37 on larvae, pupae and adults of the Mediterranean fruit fly,Ceratitis capitata(Diptera: Tephritidae)

Bulletin of Entomological Research ◽

10.1017/s000748531600078x ◽

2016 ◽

Vol 107 (2) ◽

pp. 217-224 ◽

Cited By ~ 4

Author(s):

S.E. Samri ◽

M. Baz ◽

I. Ghalbane ◽

S. El Messoussi ◽

A. Zitouni ◽

...

Keyword(s):

Insecticidal Activity ◽

Ceratitis Capitata ◽

Larval Mortality ◽

Adult Emergence ◽

Fruit Fly ◽

Mediterranean Fruit Fly ◽

The Mediterranean ◽

High Doses ◽

Layer Chromatography ◽

Significant Mortality

AbstractThe Mediterranean fruit fly (medfly),Ceratitis capitata, is considered the most important fruit pest worldwide. Its management is mainly based on the use of chemical insecticides. Although these conventional pesticides are effective at high doses, they cause considerable human health and environment problems. Thus, the aim of this study was to assess insecticidal activity of Moroccan actinobacteria againstC. capitata. A total of 12 preselected actinobacteria isolated from various Moroccan habitats were screened for their insecticidal activity against larvae, pupae and adults ofC. capitata. Four actinobacteria isolates were significantly active against the first-instar larvae, and nine were active against the medfly adult, while no significant mortality was obtained against the third-instar larval and pupal stages. Among the selected isolates, the biological screening revealed that strainStreptomycesLD-37, which showed 99.4% similarity withStreptomyces phaeochromogenes, exhibited the maximal corrected larval mortality of 98%. Moreover, the isolates AS1 and LD-37 showed the maximum significant corrected mortality against adults of 32.5 and 28.2%, respectively. The crude extract obtained from a fermented culture of strainS. phaeochromogenesLD-37 was separated into six fractions by thin layer chromatography. Fractions F3 and F4 caused a significant corrected larval mortality of 66.7 and 53.3%, respectively; whereas the maximum reduction in adult emergence was obtained with fraction F4. This finding could be useful for utilizingS. phaeochromogenesLD-37 as an alternative to chemical insecticides in pest management ofC. capitata.

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Gene discovery in an invasive tephritid model pest species, the Mediterranean fruit fly, Ceratitis capitata

BMC Genomics ◽

10.1186/1471-2164-9-243 ◽

2008 ◽

Vol 9 (1) ◽

pp. 243 ◽

Cited By ~ 44

Author(s):

Ludvik M Gomulski ◽

George Dimopoulos ◽

Zhiyong Xi ◽

Marcelo B Soares ◽

Maria F Bonaldo ◽

...

Keyword(s):

Ceratitis Capitata ◽

Gene Discovery ◽

Fruit Fly ◽

Mediterranean Fruit Fly ◽

Pest Species ◽

The Mediterranean

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Disinfestation of Western Australian Granny Smith apples by cold treatment against the egg and larval stages of the Mediterranean fruit fly (Ceratitis capitata (Wied.))

Australian Journal of Experimental Agriculture ◽

10.1071/ea9760280 ◽

1976 ◽

Vol 16 (79) ◽

pp. 280 ◽

Cited By ~ 12

Author(s):

AN Sproul

Keyword(s):

Ceratitis Capitata ◽

Cold Treatment ◽

Fruit Fly ◽

Mediterranean Fruit Fly ◽

Commercial Practice ◽

Larval Stages ◽

Experimental Programme ◽

The Mediterranean ◽

Eggs And Larvae ◽

Western Australian

In a series of tests during 1974 export grade Granny Smith apples artificially infested with eggs, young and old larvae of Mediterranean fruit fly (Ceratitis capitata (Wied.)) were cold stored at 0.5�C � 0.5�C for 14 days and at 1.5�C � 0.5�C for 16 days in simulated commercial conditions. No survivors were recorded from tests in which an estimated total of 340,200 eggs and larvae were treated. No injury to the fruit was caused by the treatments. Apples are held in cool stores after harvest at these temperatures as normal commercial practice. The experimental programme demonstrated that such commercial treatments can be applied to meet export requirements to obtain entry to quarantine areas.

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Genetic variation between strains of the Mediterranean fruit fly, Ceratitis capitata, detected by DNA fingerprinting

Genome ◽

10.1139/g92-077 ◽

1992 ◽

Vol 35 (3) ◽

pp. 528-533 ◽

Cited By ~ 9

Author(s):

David S. Haymer ◽

Donald O. Mcinnis ◽

Loretta Arcangeli

Keyword(s):

Genetic Variation ◽

Dna Fingerprinting ◽

Restriction Fragment ◽

Ceratitis Capitata ◽

Geographic Origin ◽

Fruit Fly ◽

Mediterranean Fruit Fly ◽

Great Promise ◽

Pest Species ◽

The Mediterranean

DNA fingerprinting has been used to detect genetic variation in the Mediterranean fruit fly, Ceratitis capitata. Three different probes have been identified that can be used to detect DNA restriction fragment length polymorphisms between strains of this species. The strains used in this study differ only in terms of their geographic origin or genetic background. One of the probes used is the bacteriophage vector M13, and the other two are repetitive sequences derived from the medfly genome based on a weak homology to M13. Within a strain, each probe produces a consistent restriction fragment profile that is not affected by the method or timing of DNA extraction. Between strains, when M13 is used as a probe, an average of 10% of the observable bands are polymorphic. Use of the medfly genomic sequences as a probe increases the proportion of polymorphic bands between strains up to 30%. The fact that genetic differences between even such closely related strains can be reliably detected by this method holds great promise for studies of insect pests including the ability to monitor the movements of pest species, determining the extent of genetic variation in pest populations, and in making identifications from otherwise unidentifiable material.Key words: genetic variation, Ceratitis capitata, DNA fingerprinting.

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