scholarly journals Mitochondrial Gene Sequence (COI) Reveals the Genetic Structure and Demographic History of Lymantria dispar (Lepidoptera: Erebidae: Lymantriinae) in and around China

Insects ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 146 ◽  
Author(s):  
Yao Xu ◽  
Sufang Zhang ◽  
Hongbin Wang ◽  
Mei Wang ◽  
Guohong Li
Keyword(s):  
Genetic Structure ◽  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Demographic History ◽  
Mitochondrial Gene ◽  
Dna Barcode ◽  
Full Length ◽  
Coi Gene ◽  
Gene Sequences ◽  
Gypsy Moths

The gypsy moth, Lymantria dispar, is among the most destructive quarantine pests of forests. Here, we reconstructed the genetic structure and determined the population differentiation of gypsy moths across its distribution range at different times. This information could be used to both improve the prevention and detection of gypsy moths in the field. Using 31 newly designed species-specific primers targeting fragments of 216–1102 bp, we identified 103 full-length cytochrome oxidase subunit I (COI) gene sequences from eight fresh samples and 95 L. dispar specimens collected between 1955 and 1996, mainly in China. Combining 103 full-length COI gene sequences with 146 COI gene sequences from Genbank or DNA barcode libraries, we analyzed the genetic differentiation, gene flow and haplotypes between gypsy moth populations in order to reflect the genetic structure and population dynamics of gypsy moths. We discovered 25 previously unknown haplotypes from old gypsy moth specimens. We found that the genetic diversity among gypsy moth populations (collected in the same region at different time points) was relatively high. Furthermore, the genetic structure of Chinese geographical populations (Heilongjiang, Liaoning, Beijing) in different years was distinct. Our results suggested that some gypsy moths in China showed the genetic affinity with European gypsy moths (a sub-species of gypsy moths found mainly in Europe).

scholarly journals What can be Concluded from COI Gene Data in Limited History Specimen: A sample analysis of a forest pest,Lymantria dispar

2019 ◽  
Vol 3 ◽  
Author(s):  
Hongbin Wang ◽  
Yao Xu ◽  
Guohong Li
Keyword(s):  
Genetic Differentiation ◽  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Genetic Relationships ◽  
Full Length ◽  
Coi Gene ◽  
Current Status ◽  
Pest Species ◽  
Forest Pest ◽  
Gene Sequences

Nowadays, more and more biodiversity and biogeography studies are conducted with the help of gene sequences. Fresh samples obtained using consistent collection methods can provide DNA for analysis and yield the current status of target species. As there are often no historical samples providing a timescale, it is often difficult to draw conclusions and to provide an evolutionary explanation of the observed biogeographical patterns because of a lack of evidence. The huge natural history specimen collections in museums could possibly provide this information. The gypsy moth, Lymantria dispar (Linnaeus) is a worldwide forest pest species. Our analyses of mitochondrial COI gene sequencing data in specimens from disparate locations revealed previously unknown genetic relationships in gypsy moth populations across space (in and around China) and time (1955–2012). We recovered 103 full-length COI gene sequences from eight fresh samples and from 95 Lymantria dispar collection specimens that had been captured between 1955 and 1996. Combining 103 full-length COI gene sequences with 146 COI gene sequences from Genbank (https://www.ncbi.nlm.nih.gov) or DNA barcode libraries, we analyzed the genetic differentiation, gene flow and haplotypes (special sequences from individuals) within the gypsy moth populations in order to reflect the genetic structure and population dynamics of this pest. Twenty-five previously unknown haplotypes were discovered. Regional populations from the same location, but collected at different times, showed high genetic diversity. In some geographical populations (Heilongjiang, Liaoning and Beijing populations), the genetic differentiation was greatest in 1979, but much lower in 1992 and 2012. This study is an example that shows how specimen collections can be useful to complete gaps in biodiversity studies carried out through genetic sequencing.

Genetic structure of Sakhalin spruce (Picea glehnii) in northern Japan and adjacent regions revealed by nuclear microsatellites and mitochondrial gene sequences

2014 ◽  
Vol 128 (1) ◽  
pp. 91-102 ◽  
Author(s):  
Mineaki Aizawa ◽  
Hiroshi Yoshimaru ◽  
Makoto Takahashi ◽  
Takayuki Kawahara ◽  
Hisashi Sugita ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Mitochondrial Gene ◽  
Nuclear Microsatellites ◽  
Picea Glehnii ◽  
Gene Sequences ◽  
Northern Japan

CAUSES AND ECOLOGICAL IMPLICATIONS OF EGG RETENTION IN THE GYPSY MOTH, LYMANTRIA DISPAR (L.) (LEPIDOPTERA: LYMANTRIIDAE).

1987 ◽  
Vol 119 (9) ◽  
pp. 765-771 ◽  
Author(s):  
Pedro Barbosa ◽  
Peter Martinat
Keyword(s):  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Egg Production ◽  
Pupal Development ◽  
Egg Retention ◽  
Gypsy Moths ◽  
Ecological Implications ◽  
Pupal Size ◽  
Food Consumed

AbstractThe role of starvation, the occurrence of mating, and delays and the onset of mating on the retention of eggs by the gypsy moth, Lymantria dispar (L.), were evaluated. The mating status of female gypsy moths did influence egg retention.Mating delays did not affect egg retention. Differences in the number of females retaining some eggs were found among females whose larvae had been reared on different host plants. The amount of food consumed had a significant effect on egg retention. Partial starvation had an impact on egg retention and on larval and pupal development, pupal size, and total egg production. The greater the starvation the greater the egg retention. The ecological implications of these results are discussed.

scholarly journals Molecular identification and population genetic study of Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) from Indonesia, Malaysia and Cameroon based on mitochondrial gene

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Van Basten Tambunan ◽  
Ardha Apriyanto ◽  
Walter Ajambang ◽  
Culbertson Enow Etta ◽  
Bandung Sahari ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Molecular Identification ◽  
Oil Palm ◽  
Genetic Study ◽  
Population Genetic ◽  
Mitochondrial Gene ◽  
Dna Barcode ◽  
Coi Gene ◽  
Population Genetic Study ◽  
Elaeidobius Kamerunicus

Abstract. Tambunan VB, Apriyanto A, Ajambang W, Etta CE, Sahari B, Buchori D, Hidayat P. 2020. Molecular identification and population genetic study of Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) from Indonesia, Malaysia and Cameroon based on mitochondrial gene. Biodiversitas 21: 3263-3270. Oil palm pollinating weevil Elaeidobius kamerunicus is a very important insect pollinator in oil palm plantation. However, there is still lack of information about molecular identification and population genetic study in this species. The purpose of this study was to explore the effectiveness of oil palm pollinating weevil identification using mitochondrial DNA of COI gene and to assess its genetic variation between different locations and countries. We sequenced the DNA barcode of 36 individuals of this species using the mtDNA Cytochrome Oxidase I (COI) gene to explore their genetic variation, identity and phylogenetic relationship. The COI gene sequences generated from this study were successful in identifying E. kamerunicus. Phylogenetic analysis also revealed 3 well-supported monophyletic haplogroups of E. kamerunicus population. In addition, genetic differentiation analysis revealed that most populations from Indonesia were different from Malaysian and Cameroonian populations indicating that there was a genetic variation between the population samples from these countries. The overall E. kamerunicus used in this study were geographically structured in two regions; outside Indonesia region (Cameroon and Malaysia) and Indonesia region. These results demonstrate the feasibility of using COI gene sequence for molecular identification and population genetic study of E. kamerunicus species.

Effects of Spined Soldier Bug (Hemiptera Pentatomidae) Augmentation and Sticky Barrier Bands on Gypsy Moth (Lepidoptera: Lymantriidae) Density in Oak Canopies2

1994 ◽  
Vol 29 (3) ◽  
pp. 339-346 ◽  
Author(s):  
Kevin W. Thorpe ◽  
Ralph E. Webb ◽  
Jeffrey R. Aldrich ◽  
Kathy M. Tatman
Keyword(s):  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Larval Density ◽  
Podisus Maculiventris ◽  
Egg Masses ◽  
Oak Trees ◽  
Gypsy Moths ◽  
Spined Soldier Bug ◽  
Augmentative Releases

The effects of sticky barrier bands, augmentative releases of the spined soldier bug, Podisus maculiventris (Say), and the deployment of P. maculiventris pheromone on gypsy moth, Lymantria dispar (L.), larval density in the canopy of oak trees were tested. Sticky barrier bands used alone reduced larval gypsy moth density by ≈35%. The release of 5,810 P. maculiventris nymphs per tree or the deployment of P. maculiventris pheromone to trees on which sticky barrier bands had been applied had no additional effect on gypsy moth larval density. None of the treatments affected the number of gypsy moth egg masses produced. Significantly more P. maculiventris adults were observed on trees with the pheromone, but higher numbers of nymphs were not subsequently observed on these trees. Counts of gypsy moths beneath burlap bands prior to gypsy moth pupation were about four times higher on unbanded than on banded trees, but counts of pupae beneath burlap bands did not differ between treatments.

scholarly journals The influence of the solar flux at 2.8 GHz on outbreaks of gypsy moth (Lymantria dispar L.) (Lepidoptera: Lymantriidae) in Serbia

2010 ◽  
Vol 62 (4) ◽  
pp. 1021-1025
Author(s):  
M. Milenkovic ◽  
V. Ducic ◽  
B. Milovanovic
Keyword(s):  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Solar Flux ◽  
Gypsy Moths

The connection between the solar flux at 2.8 GHz (based on mean monthly values) and the outbreaks of gypsy moths (Lymantria dispar L.) in Serbia was investigated. The researches included six outbreaks from 1952 to 2007. The average values of the solar flux ranged between 83.8 and 101.8 sfu during the outbreaks, whereas they were between 147.9 and 188.3 sfu for the periods without outbreaks. The results of the research showed that the increase in the number of gypsy moths appears when the values of the solar flux at 2.8 GHz range from 70 to 120 sfu.

scholarly journals Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

2019 ◽  
pp. 69-79
Author(s):  
Faria Farhana Rain ◽  
Abdul Jabber Howlader ◽  
Abu Faiz Md. Aslam
Keyword(s):  
Research Work ◽  
Dna Barcode ◽  
Haplotype Network ◽  
First Record ◽  
Coi Gene ◽  
Gene Sequences ◽  
Accurate Identification ◽  
Haematobia Irritans ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

Flies are considered serious pests which cause health problems of human and animal, transmitting many pathogenic microbes. Pest management programs depend on proper identification of pests. The present research work is an initiative to identify the medically and veterinary important flies based on mitochondrial COI gene sequences. Eleven species of the fly pests were identified. Among them, four fly species were the first record from Bangladesh. The phylogenetic analysis of retrieved sequences confirmed that the evolution of these species occurred from a common ancestor. Highest AT percentage (69.9%) was found in Haematobia irritans exigua and lowest GC percentage (30.4%) was found in Haematobia irritans exigua. The substitution rate of codon was found 1.88 in 1st position, 0.73 in 2nd position and 1.22 in 3rd position, respectively. Interspecific genetic divergence range of flies sequences was 5-20%. Haplotype network showed that Atylotus agrestis was mostly diverged from its common ancestors by 37 mutational steps. This research is the first molecular approach to identify the medically and veterinary important flies based on MT-COI gene sequences along with the establishment of first DNA barcode dataset for accurate identification in Bangladesh.

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