A Phylogenetic and Taxonomic Study on Xylodon (Hymenochaetales): Focusing on Three New Xylodon Species from Southern China

Three wood-inhabiting fungal species, Xylodon laceratus, X. montanus, and X. tropicus spp. nov., were collected from southern China, here proposed as new taxa based on a combination of morphological features and molecular evidence. Xylodon laceratus is characterized by the resupinate basidiomata with grandinioid hymenophore having cracked hymenial surface, and ellipsoid basidiospores; X. montanus is characterized by the annual basidiomata having the hard, brittle hymenophore with cream hymenial surface, and ellipsoid to broadly ellipsoid basidiospores (3.9–5.3 × 3.2–4.3 µm); and X. tropicus is characterized by its grandinioid hymenophore with buff to a pale brown hymenial surface and subglobose basidiospores measuring 2–4.8 × 1.6–4 µm. Sequences of ITS and nLSU rRNA markers of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony, and Bayesian inference methods. The ITS+nLSU analysis of the order Hymenochaetales indicated that the three new species clustered into the family Schizoporaceae, located in genus Xylodon; based on further analysis of ITS dataset, X. laceratus was a sister to X. heterocystidiatus; X. montanus closely grouped with X. subclavatus and X. xinpingensis with high support; while X. tropicus was retrieved as a sister to X. hastifer.

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Additions to the Knowledge of Corticioid Xylodon (Schizoporaceae, Hymenochaetales): Introducing Three New Xylodon Species from Southern China

Diversity ◽

10.3390/d13110581 ◽

2021 ◽

Vol 13 (11) ◽

pp. 581

Author(s):

Kai-Yue Luo ◽

Meng-Han Qu ◽

Chang-Lin Zhao

Keyword(s):

New Species ◽

Southern China ◽

Phylogenetic Analyses ◽

Fungal Species ◽

Molecular Evidence ◽

Similar Function ◽

Rotten Wood ◽

Three New Species ◽

Inference Methods ◽

High Support

Three wood-inhabiting fungal species, Xylodon gossypinus, X. macrosporus, and X. sinensis spp. nov. were collected from southern China, with the similar function to decompose rotten wood, which are here proposed as new taxa based on a combination of morphological features and molecular evidence. Xylodon gossypinus is characterized by the resupinate basidiomata with cotton hymenophore, and ellipsoid basidiospores; X. macrosporus is characterized by the resupinate basidiomata having the cracking hymenophore with pale yellowish hymenial surface, and larger basidiospores 8–10.5 × 7.5–9 µm; and X. sinensis differs by its grandinioid hymenial surface and subglobose basidiospores measuring as 3–5 × 2.5–4 µm. Sequences of ITS and nLSU rRNA markers of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony, and Bayesian inference methods. The ITS+nLSU analysis in Hymenochaetales revealed that the three new species clustered into the Schizoporaceae family, located in genus Xylodon; based on the ITS dataset, X. gossypinus was a sister to X. ussuriensis; X. macrosporus closely grouped with X. follis with a high support; and X. sinensis was retrieved as two sisters to X. attenuatus and X. yarraensis with a lower support.

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Steccherinum tenuissimum and S. xanthum spp. nov. (Polyporales, Basidiomycota): New species from China

PLoS ONE ◽

10.1371/journal.pone.0244520 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0244520

Author(s):

Ya-Xing Wu ◽

Jian-Rong Wu ◽

Chang-Lin Zhao

Keyword(s):

New Species ◽

Growth Habit ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Fungal Species ◽

Molecular Evidence ◽

Hyphal System ◽

Thin Walled ◽

Inference Methods ◽

High Support

Two new wood-inhabiting fungal species, Steccherinum tenuissimum and S. xanthum spp. nov. are described based on a combination of morphological features and molecular evidence. Steccherinum tenuissimum is characterized by an annual growth habit, resupinate basidiomata with an odontioid hymenial surface, a dimitic hyphal system with clamped generative hyphae, strongly encrusted cystidia and basidiospores measuring 3–5 × 2–3.5 μm. Steccherinum xanthum is characterized by odontioid basidiomata and a monomitic hyphal system with generative hyphae bearing clamp connections and covering by crystals, colourless, thin-walled, smooth, IKI–, CB–and has basidiospores measuring 2.7–5.5 × 1.8–4.0 μm. Sequences of the ITS and nLSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analyses based on molecular data of ITS + nLSU sequences showed that two new Steccherinum species felled into the residual polyporoid clade. Further investigation was obtained for more representative taxa in Steccherinum based on ITS + nLSU sequences, which demonstrated that S. tenuissimum and S. xanthum were sister to S. robustius with high support (100% BP, 100% BS and 1.00 BPP).

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Tuberculina photiniae sp. nov. (Helicobasidiales, Basidiomycota) supported by morphological characteristics and phylogenetic data

Phytotaxa ◽

10.11646/phytotaxa.317.2.3 ◽

2017 ◽

Vol 317 (2) ◽

pp. 113 ◽

Cited By ~ 1

Author(s):

YU-MEI ZHAO ◽

JIN XIE ◽

MENG-JIE LI ◽

LING ZHU ◽

TONG-XIN ZHOU ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Phylogenetic Analyses ◽

Morphological Characteristics ◽

Molecular Data ◽

Fungal Species ◽

Molecular Evidence ◽

Monophyletic Lineage ◽

Leaf Surfaces ◽

Inference Methods

A new mycoparasitic fungal species, Tuberculina photiniae sp. nov., is proposed based on morphological and molecular evidence. The species is characterized by its depressed hemispherical pale lilac to greyish purple sporodochia breaking through leaf surfaces, septated conidiophores with vinaceous lower parts and colourless upper parts, and the presence of colourless globose, subglobose or scarcely wide-ellipsoid conidia. Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analysis based on molecular data of ITS sequences showed that Tuberculina photiniae belongs to the Tuberculina-Helicobasidium clade and is closely related to H. longisporum and H. mompa. Further investigation was carried out for representative taxa in the Tuberculina-Helicobasidium group based on ITS+nLSU sequences in which the results demonstrated that the new species formed a monophyletic lineage and grouped with H. longisporum and H. mompa. Both morphological and molecular evidence confirmed the placement of the new species in the Tuberculina-Helicobasidium clade.

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Morphological and phylogenetic evidence for recognition of two new species of Hyphoderma (Basidiomycota) from southern China, with a key to all Chinese Hyphoderma

MycoKeys ◽

10.3897/mycokeys.83.69909 ◽

2021 ◽

Vol 83 ◽

pp. 145-160

Author(s):

Qian-Xin Guan ◽

Yi-Fei Li ◽

Chang-Lin Zhao

Keyword(s):

New Species ◽

Bayesian Inference ◽

Maximum Likelihood ◽

Forest Ecosystems ◽

Southern China ◽

Phylogenetic Analyses ◽

Molecular Evidence ◽

Corticioid Fungi ◽

Two New Species ◽

Inference Methods

Wood-inhabiting fungi play crucial roles as decomposers in forest ecosystems and, in this study, two new wood-inhabiting corticioid fungi, Hyphoderma puerense and H. tenuissimumspp. nov., are proposed, based on a combination of morphological features and molecular evidence. Hyphoderma puerense is characterised by effused basidiomata with smooth to floccose hymenial surface, a monomitic hyphal system with clamped generative hyphae and ellipsoid basidiospores. Hyphoderma tenuissimum is characterised by resupinate basidiomata with tuberculate to minutely-grandinioid hymenial surface, septate cystidia and cylindrical to allantoid basidiospores. Sequences of ITS and nLSU rRNA markers of the studied samples were generated and phylogenetic analyses were performed with Maximum Likelihood, maximum parsimony and Bayesian Inference methods. These analyses showed that the two new species clustered into Hyphoderma, in which H. puerense grouped with H. moniliforme and H. tenuissimum formed a singleton lineage. In addition, an identification key to Chinese Hyphoderma is provided.

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Taxonomy and Phylogeny of the Wood-Inhabiting Fungal Genus Hyphoderma with Descriptions of Three New Species from East Asia

Journal of Fungi ◽

10.3390/jof7040308 ◽

2021 ◽

Vol 7 (4) ◽

pp. 308

Author(s):

Qian-Xin Guan ◽

Chang-Lin Zhao

Keyword(s):

New Species ◽

Bayesian Inference ◽

Maximum Likelihood ◽

Phylogenetic Analyses ◽

Surface Scattering ◽

Molecular Evidence ◽

Rrna Gene ◽

Three New Species ◽

Inference Methods ◽

Fungal Genus

Three new wood-inhabiting fungi, Hyphoderma crystallinum, H. membranaceum, and H. microporoides spp. nov., are proposed based on a combination of morphological features and molecular evidence. Hyphoderma crystallinum is characterized by the resupinate basidiomata with smooth hymenial surface scattering scattered nubby crystals, a monomitic hyphal system with clamped generative hyphae, and numerous encrusted cystidia present. Hyphoderma membranaceum is characterized by the resupinate basidiomata with tuberculate hymenial surface, presence of the moniliform cystidia, and ellipsoid to cylindrical basidiospores. Hyphoderma microporoides is characterized by the resupinate, cottony basidiomata distributing the scattered pinholes visible using hand lens on the hymenial surface, presence of halocystidia, and cylindrical to allantoid basidiospores. Sequences of ITS+nLSU rRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony, and Bayesian inference methods. These phylogenetic analyses showed that three new species clustered into Hyphoderma, in which H. crystallinum was sister to H. variolosum, H. membranaceum was retrieved as a sister species of H. sinense, and H. microporoides was closely grouped with H. nemorale. In addition to new species, map to show global distribution of Hyphoderma species treated in the phylogenetic tree and an identification key to Chinese Hyphoderma are provided.

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Morphological and molecular identification of four new resupinate species of Lyomyces (Hymenochaetales) from southern China

MycoKeys ◽

10.3897/mycokeys.65.48660 ◽

2020 ◽

Vol 65 ◽

pp. 101-118

Author(s):

Jun-Zhu Chen ◽

Chang-Lin Zhao

Keyword(s):

New Species ◽

Molecular Identification ◽

Internal Transcribed Spacer ◽

Southern China ◽

Phylogenetic Analyses ◽

Its Region ◽

Molecular Data ◽

Fungal Species ◽

Molecular Evidence ◽

Thin Walled

Four new wood-inhabiting fungal species, Lyomyces bambusinus, L. cremeus, L. macrosporus and L. wuliangshanensis, are proposed based on a combination of morphological and molecular evidence. Lyomyces bambusinus is characterized by resupinate basidiomata with colliculose to tuberculate hymenial surface and broadly ellipsoid, hyaline, slightly thick-walled, smooth basidiospores. Lyomyces cremeus is characterised by resupinate basidiomata with smooth, cream hymenial surface and ellipsoid, hyaline, thin-walled to slightly thick-walled basidiospores. Lyomyces macrosporus is characterized by pruinose basidiomata with reticulate hymenial surface, presence of three kinds of cystidia and larger basidiospores (6.7–8.9 × 4.4–5.4 µm). Lyomyces wuliangshanensis is characterized by coriaceous basidiomata and ellipsoid, hyaline, slightly thick-walled, smooth basidiospores. The phylogenetic analyses based on molecular data of the internal transcribed spacer (ITS) region sequences revealed that the four new species belonged to Lyomyces. Lyomyces bambusinus grouped with L. sambuci. Lyomyces cremeus clade was sister to a clade comprised of L. microfasciculatus. Lyomyces macrosporus was sister to L. allantosporus. Lyomyces wuliangshanensis was closely related to L. mascarensis.

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Taxonomy and phylogeny of Dichostereum (Russulales), with descriptions of three new species from southern China

MycoKeys ◽

10.3897/mycokeys.40.28700 ◽

2018 ◽

Vol 40 ◽

pp. 111-126

Author(s):

Shi-Liang Liu ◽

Shuang-Hui He

Keyword(s):

New Species ◽

Southern China ◽

Phylogenetic Analyses ◽

Molecular Evidence ◽

Three New Species

Nine species of Dichostereum were subjected to phylogenetic analyses, based on a combined dataset of ITS1-5.8S-ITS2-nrLSU-tef1 sequences. The morphology of specimens collected from China and Australia were studied. Three species, D.austrosinense, D.boidinii and D.eburneum, collected from southern China, are described and illustrated as new to science, based on the morphological and molecular evidence. Dichostereumaustrosinense is characterised by the relatively large gloeocystidia (80–130 × 8–15 µm) and basidiospores (7.3–8 µm in diam.) with large warts and crests. Dichostereumboidinii is distinguished by its thick basidiomata and relatively small basidiospores (5.5–6.5 µm in diam.) with large warts and crests. Dichostereumeburneum is unique in having pale basidiomata growing on bark of living Castanopsis, abundant crystals in the context and basidiospores with dense and large ornamentations. A key to the 5 species of Dichostereum in China is given.

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Morphological and molecular identification of a new species of Eichleriella (Auriculariales, Basidiomycota) in China

Phytotaxa ◽

10.11646/phytotaxa.404.6.3 ◽

2019 ◽

Vol 404 (6) ◽

pp. 245

Author(s):

XIANG-FU LIU ◽

SHAN SHEN ◽

CHANG-LIN ZHAO

Keyword(s):

New Species ◽

Bayesian Inference ◽

Maximum Likelihood ◽

Phylogenetic Analyses ◽

Fungal Species ◽

Molecular Evidence ◽

Thin Walled ◽

Inference Methods ◽

Lsu Nrdna ◽

A New Species

A new wood-inhabiting fungal species, Eichleriella xinpingensis, is described based on a combination of morphological features and molecular evidence. The species is characterized by an annual, resupinate basidiocarps with soft leathery to ceraceous hymenial surface covered by blunt-pointed spines, a dimitic hyphal system with clamp generative hyphae, two-celled, narrowly ovoid to obconical basidia and broadly cylindrical, hyaline, thin-walled, smooth, basidiospores measuring as 6.5–10 ×3.5–4.5 µm. Sequences of ITS and LSU nrDNA regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analyses showed that E. xinpingensis belonged to the Auriculariaceae and was closely related to E. tenuicula.

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Morphological and Phylogenetic Evidence for Recognition of Two New Species of Phanerochaete from East Asia

Journal of Fungi ◽

10.3390/jof7121063 ◽

2021 ◽

Vol 7 (12) ◽

pp. 1063

Author(s):

Dong-Qiong Wang ◽

Chang-Lin Zhao

Keyword(s):

New Species ◽

Bayesian Inference ◽

Maximum Likelihood ◽

East Asia ◽

Maximum Parsimony ◽

Phylogenetic Analyses ◽

Fungal Species ◽

Molecular Evidence ◽

Two New Species ◽

Inference Methods

Two new corticioid fungal species, Phanerochaete pruinosa and P. rhizomorpha spp. nov. are proposed based on a combination of morphological features and molecular evidence. Phanerochaete pruinosa is characterized by the resupinate basidiomata with the pruinose hymenial surface, a monomitic hyphal system with simple-septate generative hyphae and subcylindrical basidiospores measuring as 3.5–6.7 × 1.5–2.7 µm. Phanerochaete rhizomorpha is characterized by having a smooth hymenophore covered by orange hymenial surface, the presence of rhizomorphs, subulate cystidia, and narrower ellipsoid to ellipsoid basidiospores. Sequences of ITS+nLSU nrRNA gene regions of the studied specimens were generated and phylogenetic analyses were performed with maximum likelihood, maximum parsimony, and Bayesian inference methods. These phylogenetic analyses showed that two new species clustered into genus Phanerochaete, in which P. pruinosa was sister to P. yunnanensis with high supports (100% BS, 100% BT, 1.00 BPP); morphologically differing by a pale orange to greyish orange and densely cracked hymenial surface. Another species P. rhizomorpha was closely grouped with P. citrinosanguinea with lower supports; morphologically having yellow to reddish yellow hymenial surface, and smaller cystidia measuring as 31–48 × 2.3–4.8 µm.

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Xylodon bambusinus and X. xinpingensis spp. nov. (Hymenochaetales) from southern China

Phytotaxa ◽

10.11646/phytotaxa.511.3.3 ◽

2021 ◽

Vol 511 (3) ◽

Author(s):

XIANG MA ◽

CHANG-LIN ZHAO

Keyword(s):

New Species ◽

Bayesian Inference ◽

Maximum Likelihood ◽

Growth Habit ◽

Southern China ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Two New Species ◽

Thin Walled ◽

Inference Methods

Two new species, Xylodon bambusinus and X. xinpingensis, are proposed based on morphological and molecular evidences. Both species share the annual growth habit, resupinate basidiomata and monomitic hyphal system with clamped, colorless generative hyphae, smooth, thin-walled basidiospores, but X. bambusinus is characterized by the smooth to tuberculate hymenial surface, presence of capitate and fusiform cystidia, broad ellipsoid basidiospores, while X. xinpingensis by the reticulate hymenophore with cream hymenial surface, and subglobose basidiospores (4.5–6 × 3.5–5 µm). Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analyses based on molecular data of ITS and ITS+nLSU sequences showed that X. bambusinus was sister to X. subclavatus, while X. xinpingensis grouped with X. astrocystidiatus and X. paradoxus. The nLSU dataset revealed that X. bambusinus grouped with X. asperus and X. brevisetus with lower supports, and that X. xinpingensis grouped with X. astrocystidiatus and X. paradoxus and then with X. rimosissimus without supports. Both morphological and molecular evidences confirmed the placement of two new species in Xylodon. Description and figures from the new species and a key to the known species of Xylodon from China are presented.

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