scholarly journals Treatment of Human Sporotrichosis Caused by Sporothrix brasiliensis

2022 ◽  
Vol 8 (1) ◽  
pp. 70
Author(s):  
Vanice Rodrigues Poester ◽  
Rossana Patricia Basso ◽  
David A. Stevens ◽  
Lívia Silveira Munhoz ◽  
Vanessa Brito de Souza Rabello ◽  
...  
Keyword(s):  
Elongation Factor ◽  
Epidemiological Data ◽  
Translation Elongation Factor ◽  
Clinical Form ◽  
Translation Elongation ◽  
Sporothrix Brasiliensis ◽  
Laboratory Confirmation ◽  
First Choice ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

We describe the successful treatment of a series of 30 zoonotic sporotrichosis cases from southern Brazil. Sporothrix brasiliensis was the species genotypically identified in all 25 confirmed cases. Five other cases were classified as probable, without laboratory confirmation, but with clinical and epidemiological data of cat-transmitted sporotrichosis. Two isolates were sequenced by translation elongation factor-1 alpha (EF1α) loci in order to compare their sequences, and both of them showed distinct genotypes from S. brasiliensis strains from other Brazilian states. Itraconazole (ITZ) or potassium iodide (KI) were the first choice treatment in 28 and 2 cases, respectively. Microdilution assay showed a wild-type profile of S. brasiliensis isolates to ITZ. However, a lack of clinical response occurred in 42% of cases, especially those treated with ITZ 100 mg/day, and treatment needed modifications, by either increased doses or antifungal combinations. Clinical cure required a mean of 187 days of treatment, which was dependent on the clinical form of the disease and age of patients. Therapy, including dosages and durations, for cutaneous forms of sporotrichosis requires re-evaluation, since cases caused by S. brasiliensis may influence treatment efficacy.

scholarly journals Four new East Asian species of Aleurodiscus with echinulate basidiospores

MycoKeys ◽  
2019 ◽  
Vol 52 ◽  
pp. 71-87
Author(s):  
Sheng-Hua Wu ◽  
Chia-Ling Wei ◽  
Yu-Ting Lin ◽  
Chiung-Chih Chang ◽  
Shuang-Hui He
Keyword(s):  
New Species ◽  
Southwest China ◽  
East Asian ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Northwest Yunnan ◽  
Elongation Factor 1 Alpha ◽  
Alpha Gene ◽  
Elongation Factor 1

Four new species of Aleurodiscus sensu lato with echinulate basidiospores are described from East Asia: A.alpinus, A.pinicola, A.senticosus, and A.sichuanensis. Aleurodiscusalpinus is from northwest Yunnan of China where it occurs on Rhododendron in montane habitats. Aleurodiscuspinicola occurs on Pinus in montane settings in Taiwan and northwest Yunnan. Aleurodiscussenticosus is from subtropical Taiwan, where it occurs on angiosperms. Aleurodiscussichuanensis is reported from southwest China on angiosperms in montane environments. Phylogenetic relationships of these four new species were inferred from analyses of a combined dataset consisting of three genetic markers, viz. 28S, nuc rDNA ITS1-5.8S-ITS2 (ITS), and a portion of the translation elongation factor 1-alpha gene, TEF1.

scholarly journals First report of Fusarium solani causing stem rot of Dracaena in Iran

2016 ◽  
Vol 56 (1) ◽  
pp. 100-103 ◽  
Author(s):  
Mostafa Abedi-Tizaki ◽  
Doustmorad Zafari ◽  
Jamal Sadeghi
Keyword(s):  
Fusarium Solani ◽  
Elongation Factor ◽  
Stem Rot ◽  
Pathogenicity Test ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
First Report ◽  
Brown Discoloration ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

Abstract In July 2013, symptoms of stem rot were observed in the Dracaena sanderiana cuttings in greenhouses of Mahallat County, Markazi Province, Iran. The symptoms first appeared as severe wilting. Later, leaves became brown and necrotic. Symptoms on the cuttings were observed as rotted areas on the middle of the stems. The cortical tissues of the plants showed a distinct brown discoloration. Eventually, the infected plants died. The pathogen was isolated from Dracaena stems and identified as F. solani by a fragment of the translation elongation factor 1-alpha (EF-1α) gene. Fusarium solani was confirmed by a pathogenicity test, and the causal agent was re-isolated from infected D. sanderiana plants. To the best of our knowledge, this is the first report of stem rot caused by F. solani on the cuttings of D. sanderiana.

Botryosphaeria sinensia sp nov., a new species from China

Phytotaxa ◽  
2016 ◽  
Vol 245 (1) ◽  
pp. 43 ◽  
Author(s):  
Yupei Zhou ◽  
Zhipeng Dou ◽  
Wei He ◽  
Xiaodong Zhang ◽  
Ying Zhang
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Elongation Factor ◽  
Intergenic Spacer ◽  
Translation Elongation Factor ◽  
Sequence Comparisons ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
A New Species

A new Botryosphaeria species (B. sinensia) is described and illustrated from China. It is characterized by erumpent, botryose clustered ascostromata, ostiolate ascomata, bitunicate, 8-spored and clavate asci, filiform, rarely branched pseudoparaphyses, hyaline, 1-celled, fusiform ascospores, and a Fusicoccum asexual morph. The hyaline old and discharged conidia with 1–2 septa, broader spermatia formed in the culture and smaller ascostroma readily distinguished it from B. dothidea and other species in Botryosphaeria. The intergenic spacer (ITS)  and translation elongation factor 1-alpha (EF1) DNA sequence analysis results also support its separation from other Botryosphaeria species. Its relationships with other species of Botryosphaeria are also discussed based on morphology and DNA sequence comparisons.

scholarly journals Morphology and Phylogeny of Gnomoniopsis (Gnomoniaceae, Diaporthales) from Fagaceae Leaves in China

2021 ◽  
Vol 7 (10) ◽  
pp. 792
Author(s):  
Ning Jiang ◽  
Hermann Voglmayr ◽  
Dan-Ran Bian ◽  
Chun-Gen Piao ◽  
Sheng-Kun Wang ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Elongation Factor ◽  
Nuclear Ribosomal Dna ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Its Regions ◽  
Plant Families ◽  
Internally Transcribed Spacer ◽  
Elongation Factor 1

Gnomoniopsis (Gnomoniaceae, Diaporthales) is a well-classified genus inhabiting leaves, branches and fruits of the hosts in three plant families, namely Fagaceae, Onagraceae and Rosaceae. In the present study, eighteen Gnomoniopsis isolates were obtained from diseased leaves of Fagaceae hosts collected from Fujian, Guangdong, Hainan, Henan, Jiangxi and Shaanxi provinces in China. Morphology from the cultures and phylogeny based on the 5.8S nuclear ribosomal DNA gene with the two flanking internally transcribed spacer (ITS) regions, the translation elongation factor 1-alpha (tef1) and the beta-tubulin (tub2) genes were employed to identify these isolates. As a result, seven species were revealed, viz. Gnomoniopsis castanopsidis, G. fagacearum, G. guangdongensis, G. hainanensis, G. rossmaniae and G. silvicola spp. nov, as well as a known species G. daii. In addition, G. daii was firstly reported on the host Quercus aliena.

scholarly journals Translation elongation factor 1-alpha gene as a marker for diagnosing of Candida onychomycosis

2020 ◽  
Author(s):  
Keyvan Pakshir ◽  
Forough Farazmand ◽  
Farnoush Ghasemi ◽  
Hossein Mirhend ◽  
Kamiar Zomorodian ◽  
...  
Keyword(s):  
Candida Species ◽  
Simple Procedure ◽  
Elongation Factor ◽  
Sequence Difference ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Base Pair Fragment ◽  
Successful Amplification ◽  
Elongation Factor 1

Background and Purpose: Culture-based identification methods have been the gold standard for the diagnosis of candidal onychomycosis. Molecular technologies, such as polymerase chain reaction (PCR) assays, can provide an alternative for the rapid detection of Candida species. The present study was conducted to investigate a pan-Candida PCR assay based on the translation elongation factor 1-alpha (TEF-1α) gene for the detection of the most prevalent pathogenic Candida species. Materials and Methods: For the purpose of the study, an optimized pan-Candida PCR primer pair was designed, and the target was amplified and sequenced. The analytical and clinical diagnostic performance of the designed primers was tested using 17 reference strains, 137 nail scrapings suspected of onychomycosis, and 10 healthy nail specimens. Results: The use of the universal pan-Candida primers designed on TEF-1α gene resulted in the successful amplification of a 270-base pair fragment in all Candida species tested, except for C. glabrata, and reacted neither with other fungi nor with E. coli. The sequence difference count matrix showed poor insertion/deletion differences (0-2 nt) among Candida species. Among 137 nail specimens, 35% (n=48), 30.7% (n=42), and 40.1% (n=55) of the samples were found to be positive by direct microscopy, culture, and pan-Candida PCR, respectively. Conclusion: Based on the findings, the PCR-based detection targeting the DNA TEF-1α gene is a rapid and simple procedure for the diagnosis of candidal onychomycosis directly from nail sample.

New species of Nectria and Thyronectria from Xinjiang, China

Phytotaxa ◽  
2020 ◽  
Vol 433 (4) ◽  
pp. 253-264
Author(s):  
RONG MA ◽  
SHENG-NAN LI ◽  
YING ZHAO ◽  
MIN WANG ◽  
THEMIS J. MICHAILIDES ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Alpha Actin ◽  
First Time ◽  
Elongation Factor 1

Nectria berberidis sp. nov. and Thyronectria berberidicola sp. nov. isolated from Berberis heteropoda in Xinjiang Uygur Autonomous Region, China, are described and illustrated. Nectria berberidis is characterized by clavate asci (50–87 × 8–12 μm) with ellipsoidal to fusiform, 1-septate ascospores. Thyronectria berberidicola is characterized by clavate asci (117–25.9 × 63.7–117.9 μm) with ellipsoidal to fusiform ascospores that have 5–8 transverse septa and 1(–2) longitudinal septum. Ascospores bud to produce hyaline, bacillar ascoconidia. Phylogenetic analyses based on alpha-actin (ACT), the internal transcribed spacer (ITS), the large nuclear ribosomal RNA subunit (LSU), translation elongation factor 1-alpha (TEF1) and the β-tubulin (TUB) sequence data revealed that isolates of N. berberidis and T. berberidicola form a distinct clade within Nectria and Thyronectria, respectively. In addition, Nectria nigrescens is reported for the first time in China.

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